A common allosteric mechanism regulates homeostatic inactivation of auxin and gibberellin

Takehara, Shoichiro; Sakuraba, Shun; Mikami, Bunzo; Yoshida, Hideki; Yoshimura, Hisako; Itoh, Aya; Endo, Masaki; Watanabe, Nobuhisa; Nagae, T.; Matsuoka, Makoto; Ueguchi-Tanaka, Miyako

doi:10.1038/s41467-020-16068-0

Cited by 34 publications

(35 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…GA 4 also promoted dimerization of the C 20 -GA2ox OsGA2ox6, for which it is not the preferred substrate. Remarkably, Takehara et al. 2020 found a similar mechanism, involving dimerization of the auxin catabolic enzyme indoleacetic acid oxidase, also a 2-ODD, in the presence of its substrate.…”

Section: Inactivationmentioning

confidence: 70%

“…The recent determination of the X-ray crystal structure of the rice C 19 -GA2ox OsGA2ox3 revealed that it formed a tetramer in the presence of its substrate GA 4 , with the monomers linked via two GA 4 molecules and two disulfide bridges ( Takehara et al. 2020 ).…”

Section: Inactivationmentioning

confidence: 99%

“…As discussed above, feedback regulation may also occur at the protein level ( Lee and Zeevaart 2007 ) and, although it is more difficult to study, it warrants further investigation. In addition, allosteric regulation has been described for CPS, the activity of which is suppressed synergistically by Mg 2+ and GGPP ( Prisic and Peters 2007 ), and for OsGA2ox3, which is activated by its substrate GA 4 ( Takehara et al. 2020 ), both mechanisms contributing to GA homeostasis.…”

Section: Regulation Of Ga Metabolismmentioning

confidence: 99%

See 2 more Smart Citations

The Current Status of Research on Gibberellin Biosynthesis

Hedden

2020

Plant and Cell Physiology

192

134

View full text Add to dashboard Cite

Abstract Gibberellins are produced by all vascular plants as well as several fungal and bacterial species that associate with plants as pathogens or symbionts. In the 60 years since the first experiments on the biosynthesis of gibberellic acid in the fungus Fusarium fujikuroi, research on gibberellin biosynthesis has advanced to provide detailed information on the pathways, biosynthetic enzymes and their genes in all three kingdoms, in which the production of the hormones evolved independently. Gibberellins function as hormones in plants, affecting growth and differentiation in organs in which their concentration is very tightly regulated. Current research in plants is focussed particularly on the regulation of gibberellin biosynthesis and inactivation by developmental and environmental cues, and there is now considerable information on the molecular mechanisms involved in these processes. There have also been recent advances in understanding gibberellin transport and distribution and their relevance to plant development. This review describes our current understanding of gibberellin metabolism and its regulation, highlighted the more recent advances in this field.

show abstract

Section: Inactivationmentioning

confidence: 70%

Section: Inactivationmentioning

confidence: 99%

Section: Regulation Of Ga Metabolismmentioning

confidence: 99%

See 1 more Smart Citation

The Current Status of Research on Gibberellin Biosynthesis

Hedden

2020

Plant and Cell Physiology

192

134

View full text Add to dashboard Cite

show abstract

“…To determine the functional similarity of hormone biosynthetic and metabolism 2OGD proteins of tomato with those of Arabidopsis and rice, we performed multiple sequence alignments and motif composition analysis. Two 2OGD-family proteins of known three-dimensional structure—OsGA2ox3 and OsDAO [ 17 ], and seven hormone biosynthetic and metabolism 2OGD-family proteins—AtGA20ox1, AtGA3ox1, AtGA2ox7, SlACO1, AtJOX1, AtDMR6, and AtLBO1—which have been functionally characterized were aligned to identify conserved domains or motifs in 2OGD family. The result showed that the above 2OGD proteins had the HxD…H and RxS/T conserved motifs in OsGA2ox3 and OsDAO ( Figure 3 a), which recruit Fe(II) as a cofactor and co-substrate.…”

Section: Resultsmentioning

confidence: 99%

Identification and Expression Analysis of Hormone Biosynthetic and Metabolism Genes in the 2OGD Family for Identifying Genes That May Be Involved in Tomato Fruit Ripening

Ding

Wang

Xue

et al. 2020

IJMS

View full text Add to dashboard Cite

Phytohormones play important roles in modulating tomato fruit development and ripening. The 2-oxoglutarate-dependent dioxygenase (2OGD) superfamily containing several subfamilies involved in hormone biosynthesis and metabolism. In this study, we aimed to identify hormone biosynthesis and metabolism-related to 2OGD proteins in tomato and explored their roles in fruit development and ripening. We identified nine 2OGD protein subfamilies involved in hormone biosynthesis and metabolism, including the gibberellin (GA) biosynthetic protein families GA20ox and GA3ox, GA degradation protein families C19-GA2ox and C20-GA2ox, ethylene biosynthetic protein family ACO, auxin degradation protein family DAO, jasmonate hydroxylation protein family JOX, salicylic acid degradation protein family DMR6, and strigolactone biosynthetic protein family LBO. These genes were differentially expressed in different tomato organs. The GA degradation gene SlGA2ox2, and the auxin degradation gene SlDAO1, showed significantly increased expression from the mature-green to the breaker stage during tomato fruit ripening, accompanied by decreased endogenous GA and auxin, indicating that SlGA2ox2 and SlDAO1 were responsible for the reduced GA and auxin concentrations. Additionally, exogenous gibberellin 3 (GA3) and indole-3-acetic acid (IAA) treatment of mature-green fruits delayed fruit ripening and increased the expression of SlGA2ox2 and SlDAO1, respectively. Therefore, SlGA2ox2 and SlDAO1 are implicated in the degradation of GAs and auxin during tomato fruit ripening.

show abstract

“…GA catabolism is largely dependent on deactivating 2β-hydroxylation of bioactive GAs and their precursors, mediated by GA 2-oxidases (GA2oxs). The Arabidopsis genome encodes five C 19 -GA 2oxs ( GA2ox 1 , 2 , 3 , 4 , and 6 ) involved in depleting pools of bioactive GAs and their immediate precursors (Rieu et al 2008; Martínez-Bello et al 2015; Takehara et al 2020).…”

Section: Introductionmentioning

confidence: 99%

AnHB40-Jungbrunnen1-GA 2-OXIDASEregulatory module for gibberellin homeostasis in Arabidopsis

Dong

Tarkowská

Sedaghatmehr

et al. 2021

Preprint

View full text Add to dashboard Cite

The phytohormones gibberellins (GAs) play fundamental roles in almost every aspect of plant growth and development. Although there is good knowledge about GA biosynthetic and signaling pathways, factors contributing to the mechanisms homeostatically controlling GA levels remain largely unclear. Here, we demonstrate that homeobox transcription factor HB40 of the HD-Zip family in Arabidopsis thaliana regulates GA content at two additive control levels. We show that HB40 expression is induced by GA and in turn reduces the levels of endogenous bioactive GAs by a simultaneous reduction of GA biosynthesis and increased GA deactivation. Hence, HB40 overexpression leads to typical GA-deficiency traits, such as small rosettes, reduced plant height, delayed flowering, and male sterility. In contrast, a loss-of-function hb40 mutation enhances GA-controlled growth. Genome-wide RNA-sequencing combined with molecular-genetic analyses revealed that HB40 directly activates transcription of JUNGBRUNNEN1 (JUB1), a key TF repressing growth by suppressing GA biosynthesis and signaling. HB40 also represses genes encoding GA 2-oxidases (GA2oxs) which are major GA catabolic enzymes. The effect of HB40 is ultimately mediated through induction of nuclear growth-repressing DELLA proteins. Our results thus uncover an important role of the HB40/JUB1/GA2ox/DELLA network in controlling GA homeostasis during plant growth.

show abstract

A common allosteric mechanism regulates homeostatic inactivation of auxin and gibberellin

Cited by 34 publications

References 48 publications

The Current Status of Research on Gibberellin Biosynthesis

The Current Status of Research on Gibberellin Biosynthesis

Identification and Expression Analysis of Hormone Biosynthetic and Metabolism Genes in the 2OGD Family for Identifying Genes That May Be Involved in Tomato Fruit Ripening

AnHB40-Jungbrunnen1-GA 2-OXIDASEregulatory module for gibberellin homeostasis in Arabidopsis

Contact Info

Product

Resources

About