A combined microscopic-molecular method for the diagnosis of strongylid infections in sheep

Bott, Nathan J.; Campbell, Bronwyn E.; Beveridge, Ian; Chilton, Neil B.; Rees, Dianne; Hunt, Peter W.; Gasser, Robin B.

doi:10.1016/j.ijpara.2009.03.002

Cited by 98 publications

(122 citation statements)

References 51 publications

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“…The first and second internal transcribed spacers (ITS1 and ITS2) are comparatively more variable than the rRNA coding regions and have thus been used for differentiation of closely related species (1,4,6,10,22,23). We PCR amplified and sequenced ITS1 from A. costaricensis (two laboratory strains from Costa Rica and Brazil), A. vasorum (from naturally infected hosts in United Kingdom), and A. cantonensis from three geographical regions (one laboratory strain from Japan plus nine environmental isolates from Hawaii and New Orleans, LA) to assess the variability of this potential PCR target.…”

mentioning

confidence: 99%

Improved Molecular Detection ofAngiostrongylus cantonensisin Mollusks and Other Environmental Samples with a Species-Specific Internal Transcribed Spacer 1-Based TaqMan Assay

Qvarnström

Silva

Teem

et al. 2010

Appl Environ Microbiol

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mentioning

confidence: 99%

Improved Molecular Detection ofAngiostrongylus cantonensisin Mollusks and Other Environmental Samples with a Species-Specific Internal Transcribed Spacer 1-Based TaqMan Assay

Qvarnström

Silva

Teem

et al. 2010

Appl Environ Microbiol

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“…The development of the multiplex PCR gave the opportunity to diagnose in a single reaction different nematode genera and/or species. Real-time PCR assess the quantification of the DNA which indicates the proportion of each genus or species in given sample [7].…”

Section: Different Molecular Tools Genomicsmentioning

confidence: 99%

Proteomics and Genomics in Veterinary Parasitology – A Diagnostic Tool

B¹,

Sivajothi²

2017

J Mol Biomark Diagn

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A different number of modern molecular technologies, genomics and proteomics in applied areas of veterinary parasitology are increasing rapidly. To assess the molecular and genetic host parasitic interaction different advanced molecular tools are required and it is useful in the diagnosis of parasitic diseases. The important molecular tool for identification and diagnosis of parasites is polymerase chain reaction as well as there are other so many applications. In genomics, species-specific probes or primers are available along with conventional techniques. These techniques will give more information on the DNA sequences of parasites will reveal many more unique sequences which can be used for identification, diagnosis of parasitic infections. Genomics is crucial for the assessment of evolutionary biology, molecular epidemiology, physiology of parasites and vaccine development. These techniques may also help in the selection of genetically resistant hosts to parasite infection and discovery of new antiparasitic drugs, improvements to existing chemotherapeutic families and monitoring of drug resistance. From the last decade utilization of the aspects of molecular biology and their applications increased and it is essential to teach and train the veterinary parasitologists.…

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“…Such approach, associated with the development of the Real-time PCR assays, created the opportunity to refine the diagnosis incorporating quantification of the DNA in a given sample, i.e., indicating the proportion of each genus or species in each sample (SAMSON-HIMMELSTJERNA et al, 2002;BOTT et al, 2009). In this case, one problem to take into consideration is the development of the eggs in the fecal samples.…”

Section: Influence Of the Rate Of Infection And The Immune Response Omentioning

confidence: 99%

Advances in the diagnosis of the gastrointestinal nematode infections in ruminants

Amarante

2016

Braz. J. Vet. Res. Anim. Sci.

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Enumeration of nematode eggs in fecal samples using the McMaster technique and morphological identification of third stage larvae from fecal cultures have been extensively used with satisfactory results in the diagnosis of the gastrointestinal nematode infections in ruminants. In order to improve sensitivity and accuracy, other approaches for quantification of eggs have been employed, like the FLOTAC and Mini-FLOTAC techniques. Results obtained in different studies indicate that fecal egg counts are a reliable measure of the size of the worm burden. However, the immunological status of the animals should be taken into consideration to interpret the results of the fecal examination. Molecular techniques have also been useful in the diagnosis of parasitic diseases. The ultimate in diagnosis has been the development of robotic platforms that enable separation of eggs from feces. Because manipulation is minimal, good quality DNA from eggs is obtained, which is used for amplification, and finally, produces a result indicating the degree of the infection by the different parasite species in mix infections. The ideal method should be reliable, friendly to non-experts and quick to perform. With the advance in robotics, bioinformatics and molecular biology, methods with such characteristics are expected to become available and affordable to be used in laboratories for the routine diagnosis of gastrointestinal nematodes of ruminants. Keywords: Haemonchus. Cooperia. EPG. PCR. ResumoA quantificação de ovos de nematoides em amostras de fezes e a identificação morfológica das larvas de terceiro estágio a partir de culturas de fezes têm sido amplamente utilizadas, com resultados satisfatórios para o diagnóstico das infecções por nematoides gastrintestinais em ruminantes. Com o intuito de melhorar a sensibilidade na detecção dos ovos, outras técnicas, tais como Flotac e Miniflotac, têm sido empregadas. Resultados obtidos em diferentes estudos indicam que a contagem de ovos nas fezes é uma medida confiável e representativa da magnitude da carga parasitária. No entanto, o estado imunológico dos animais deve ser considerado na interpretação dos resultados coproparasitológicos. As técnicas moleculares também têm sido úteis no diagnóstico das enfermidades parasitárias. Os maiores avanços no diagnóstico têm sido propiciados pelo desenvolvimento de plataformas robóticas, que separam os ovos das fezes com pouca manipulação, propiciando a obtenção de DNA de ovos de boa qualidade, o que resulta em uma amplificação que possibilita indicar o grau da infecção pelas diferentes espécies de parasitas em infecções mistas. O método ideal deve ser preciso, rápido e de fácil execução por não-especialistas. Com o avanço da robótica, bioinformática e biologia molecular, espera-se que equipamentos com tais características se tornem disponíveis e acessíveis para serem utilizados na rotina laboratorial para o diagnóstico de nematoides gastrintestinais de ruminantes. Palavras-chave: Haemonchus. Cooperia. EPG. PCR.

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A combined microscopic-molecular method for the diagnosis of strongylid infections in sheep

Cited by 98 publications

References 51 publications

Improved Molecular Detection ofAngiostrongylus cantonensisin Mollusks and Other Environmental Samples with a Species-Specific Internal Transcribed Spacer 1-Based TaqMan Assay

Improved Molecular Detection ofAngiostrongylus cantonensisin Mollusks and Other Environmental Samples with a Species-Specific Internal Transcribed Spacer 1-Based TaqMan Assay

Proteomics and Genomics in Veterinary Parasitology – A Diagnostic Tool

Advances in the diagnosis of the gastrointestinal nematode infections in ruminants

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