2012
DOI: 10.1590/s1517-83822012000100029
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A combined enrichment/polymerase chain reaction based method for the routine screening of Streptococcus agalactiae in pregnant women

Abstract: Group B Streptococcus (GBS) is the most common cause of life-threatening infection in neonates. Guidelines from CDC recommend universal screening of pregnant women for rectovaginal GBS colonization. The objective of this study was to compare the performance of a combined enrichment/PCR based method targeting the atr gene in relation to culture using enrichment with selective broth medium (standard method) to identify the presence of GBS in pregnant women. Rectovaginal GBS samples from women at ≥36 weeks … Show more

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Cited by 12 publications
(15 citation statements)
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“…Regardless, the colonization rates observed in this study (22.5% to 26%) are consistent with those detected worldwide 2,6,8,14,16,18,20 .…”
Section: Tablesupporting
confidence: 91%
See 1 more Smart Citation
“…Regardless, the colonization rates observed in this study (22.5% to 26%) are consistent with those detected worldwide 2,6,8,14,16,18,20 .…”
Section: Tablesupporting
confidence: 91%
“…The electrophoresis was performed according to the method of Sambrook et al [18][19][20] using 2% agarose gel. The amplification products were detected using 1:5 ratio of the amplified reaction mixture with 1μL GelRed (Nucleic Gel Stain, BioAmerica, Inc.) and visualized under ultraviolet light.…”
Section: Pcr Assaymentioning
confidence: 99%
“…GBS colonization rates were 19.7% and 30.5% when using culture and PCR methods, respectively. In Brazil, recent studies report a prevalence ranging from 15.9% to 22.5%, when using the culture method [27][28][29], and from 26.9% to 35.9% when using the PCR method [28,29]. These rates are very similar to observed in this study.…”
Section: Discussionsupporting
confidence: 89%
“…One example is MB tests for detecting group B streptococci (GBS) in vaginal and/or anal canal in parturient women, with a reliable result within 2 h [32][33][34][35][36]. These tests can dramatically reduce the risk to miss women who were not screened by culture before admission, or screened too late to have the result available at the time of admission.…”
Section: Nucleic Acid Amplifications Tests and Point-of-carementioning
confidence: 99%