A combination of Tropilaelaps mercedesae and imidacloprid negatively affects survival, pollen consumption and midgut bacterial composition of honey bee

Ma, Shilong; Yang, Yang; Fu, Zhanli; Diao, Qingyun; Wang, Mengyue; Luo, Qin; Wang, Xing; Dai, Pingli

doi:10.1016/j.chemosphere.2020.129368

Cited by 13 publications

(12 citation statements)

References 56 publications

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“…In addition to the sublethal effect and development impairment on colonies, insecticides have significant adverse effects on pollinating bees' gut bacterial composition and diversity [25][26][27]. Exposure to imidacloprid affected the gut microbiota composition of honeybees, and the abundance of Lactobacillus Firm-5 was significantly decreased [28].…”

Section: Introductionmentioning

confidence: 99%

Impacts of Imidacloprid and Flupyradifurone Insecticides on the Gut Microbiota of Bombus terrestris

et al. 2022

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Bumblebees are important pollinators for crops and wild flowering plants. Various pesticides have threatened the abundance and diversity of bumblebees. In addition to direct sublethal effects, pesticides may alter the gut microbial communities of bees. Imidacloprid and flupyradifurone insecticides both bind to the nicotinic acetylcholine receptor. However, the latter was assumed to be harmless for honeybees and can even be applied to flowering crops. In this study, we assessed the impacts of these two pesticides on queenless microcolonies and the gut microbiota of Bombus terrestris. We found that 10 μg/L imidacloprid significantly impeded syrup consumption, and postponed the egg-laying period, larvae, and pupae development. It decreased the relative abundance of the bumblebee-specific symbionts, Apibacter and Lactobacillus Firm-5. On the contrary, 10 μg/L flupyradifurone did not reduce syrup consumption, block larvae and pupae development in bumblebees. Although no significant phenotypes were observed, PICRUST revealed that flupyradifurone suppressed pathways, involving carbohydrate metabolism, nucleotide metabolism, translation, and membrane transport. Our findings suggest the appropriate use of this new pesticide may be considered safe for bumblebees, but the underlying mechanism warrants further investigation.

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Section: Introductionmentioning

confidence: 99%

Impacts of Imidacloprid and Flupyradifurone Insecticides on the Gut Microbiota of Bombus terrestris

et al. 2022

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“…In each colony, broods at the same age were obtained by caging the queen on an empty frame for 24 h to lay eggs. The comb with eggs was divided into small pieces and fixed on a small frame to constitute special comb, and then put back into the original colony [ 19 ].…”

Section: Methodsmentioning

confidence: 99%

“…The reproduction of the T. mercedesae in A. mellifera colonies was according to the method reported by Khongphinitbunjong et al [28]. In short, one foundress of Tropilaelaps was introduced into a brood cell with a 5th larval instar after sealing, and mites were inoculated into newly sealed brood cells [19]. All inoculum mites were randomly obtained from highly infested A. mellifera colonies.…”

Section: Honey Bees and Mitesmentioning

confidence: 99%

“…Infestation of T. mercedesae lead to a reduction in weight and longevity, and increase of the sugar syrup and pollen consumption in adult worker bees [4,18]. The combination of T. mercedesae infestation and imidacloprid 50 μg/L exposure reduced survival and increased pollen consumption of A. mellifera [19].…”

Section: Introductionmentioning

confidence: 99%

“…mercedesae infestation and imidacloprid 50 μg/L exposure reduced survival and increased pollen consumption of A . mellifera [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Tropilaelaps mercedesae parasitism changes behavior and gene expression in honey bee workers

Gao

Wang

et al. 2021

PLoS Pathog

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Tropilaelaps mercedesae is one of the most problematic honey bee parasites and has become more threatening to the beekeeping industry. Tropilaelaps can easily parasitize immature honey bees (larvae and pupae) and have both lethal and sublethal effects on the individual worker bees. Our study for the first time experimentally assessed the effects of T. mercedesae on olfactory learning, flight ability, homing ability as well as transcriptional changes in parasitized adult honey bees. T. mercedesae infestation had negative impacts on olfactory associated function, flight ability, and homing rate. The volume of the mushroom body significantly increased in infested honey bees, which may be correlated to the lower sucrose responsiveness as well as lower learning ability in the infested bees. The gene expression involved in immune systems and carbohydrate transport and metabolism were significantly different between infested bees and non-infested bees. Moreover, genes function in cell adhesion play an essential role in olfactory sensory in honey bees. Our findings provide a comprehensive understanding of European honey bees in response to T. mercedesae infestation, and could be used to further investigate the complex molecular mechanisms in honey bees under parasitic stress.

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Chlorothalonil alters the gut microbiota and reduces the survival of immature honey bees reared in vitro

Han

Wang

et al. 2022

Pest Management Science

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BACKGROUND Chlorothalonil is a nonsystemic fungicide, and it is one of the most widely detected pesticides in bee hives. The effect of chlorothalonil on the survival, weight, and gut microbiota of immature Apis mellifera L. reared in vitro was studied. RESULTS Larvae were fed 1, 2, 4, 8, and 16 μg/mL chlorothalonil and compared with larvae fed the negative control (diet without any additives), positive control (45 mg/L dimethoate), and solvent control (2% acetone). Compared with the control groups, the survival of the 2, 4, 8, and 16 μg/mL chlorothalonil treatments was significantly reduced. The no‐observed‐adverse‐effect concentration of chlorothalonil was 1 μg/mL. Chlorothalonil had no significant effect on larval weight. The gut bacterial community composition of newly emerged bees was determined by PacBio 16S rDNA gene sequencing. linear discriminant analysis effect size (LEFSe) analysis showed that Pseudomonadales and Burkholderiales were affected by exposure to chlorothalonil. CONCLUSION Chlorothalonil reduced the survival of honey bee larvae and altered the gut microbiota of newly emerged bees. The risk of pesticides to honey bees is related to their toxicity and exposure dose.

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A combination of Tropilaelaps mercedesae and imidacloprid negatively affects survival, pollen consumption and midgut bacterial composition of honey bee

Cited by 13 publications

References 56 publications

Impacts of Imidacloprid and Flupyradifurone Insecticides on the Gut Microbiota of Bombus terrestris

Impacts of Imidacloprid and Flupyradifurone Insecticides on the Gut Microbiota of Bombus terrestris

Tropilaelaps mercedesae parasitism changes behavior and gene expression in honey bee workers

Chlorothalonil alters the gut microbiota and reduces the survival of immature honey bees reared in vitro

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