A combination of Citrus reticulata peel and black tea inhibits migration and invasion of liver cancer via PI3K/AKT and MMPs signaling pathway

Wen, Shuai; Sun, Lin; An, Ran; Zhang, Wenji; Xiang, Limin; Li, Qiuhua; Lai, Xingfei; Huo, Mengen; Li, Dongli; Sun, Shili

doi:10.1007/s11033-019-05157-z

Cited by 17 publications

(13 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In line with Kim, et al (2005) reported that Citrus reticulata had an apoptosis effect on Human Gastric Cancer Cells SNU-668 by the high expression on Bax, caspase 3 and low expression of BCl2 on dose and time dependent manner. The inhibition of migration, invasion and proliferation liver cancer cell via down regulation of MMP-2/9, N-cadherin, and vimentin induced by combination between Citrus reticulate peel and black tea (Wen, et al, 2019). Similar result showed by Chang, et al (2015), that Citrus reticulate has inhibitory effect on ovarian cancer metastasis, also inhibit TGF-β1-induced EMT through the canonical TGF-β1-SMAD-Snail/Slug axis.…”

Section: Discussionsupporting

confidence: 65%

Ethanolic Extract of Citrus reticulata Peel Inhibits the Migration of WiDr Colon Cancer Cells

Astuti

Primasari

2020

Indones J Cancer Chemoprevent

View full text Add to dashboard Cite

Colorectal cancer is third rank on the cancer cases in Indonesia. To cure the cancer needs big cost and lot of effort. On the other side, the side effect of medicine or chemotherapy on patient need to reduce. Cancer cell spread to other tissue based on its migration and invasion ability. Citrus reticulata peel contains flavonoid such as Tangeretin and Nobiletin, both of this compounds have anticancer activity. The aims of this study is to reveals the potency of ethanol extract of Citrus reticulata peel on the inhibition of migration on WiDr colon cancer cells. The toxicity of ethanol extract of Citurs reticulata peel on WiDr colon cancer line was measured using 3-(4,5-dimethyltiazol-2-il)-2,5-diphenyltrazolium bromide (MTT) assay and investigate the cell migration was using scratch wound healing assay. The ethanol extract of Citrus reticulata peel showed the value of inhibitory concentration 50 (IC50) was 184.5 μg/mL, this result categorize as moderate cytotoxic. Meanwhile the migration assay showed that the deceleration of migration occurred on 0.5 IC50, 0.33 IC50 and 0.25 IC50 during 24 h and 36 h incubation, event thought there were not significant different (p>0.05). The ethanol extract of Citrus reticulata peel has a potential migration inhibition on WiDr cell line.Keywords: Citrus reticulata, WiDr cell line, migration

show abstract

Section: Discussionsupporting

confidence: 65%

Ethanolic Extract of Citrus reticulata Peel Inhibits the Migration of WiDr Colon Cancer Cells

Astuti

Primasari

2020

Indones J Cancer Chemoprevent

View full text Add to dashboard Cite

show abstract

“…37 Generally, tumor suppressor drugs can not only promote tumor cell apoptosis, but also inhibit tumor cell invasion. 38,39 In this study, we found that Omega-3PUFA significantly inhibited the invasion of colorectal cancer cells. These results indicate that Omega-3 PUFA may be used for the treatment of highly malignant colorectal cancer.…”

Section: Discussionmentioning

confidence: 51%

<p>Omega-3PUFA Attenuates MNU-Induced Colorectal Cancer in Rats by Blocking PI3K/AKT/Bcl-2 Signaling</p>

Huang

Chun-an

Cai

et al. 2020

OTT

View full text Add to dashboard Cite

Background: Omega 3 polyunsaturated fatty acid (Omega-3PUFA) is one of the essential nutrients for human body involved in intracellular metabolic regulation and cell signaling. Previous studies have shown that Omega-3PUFA is involved in the pathogenesis of digestive system tumors, including colorectal cancer (CRC), however, the effects of Omega-3PUFA on CRC has not been fully elucidated. In the current study, we evaluated whether Omega-3PUFA can alleviate N-methyl-N-nitrosourea(MNU) induced CRC in a rat model and illustrated the potential mechanism. Methods: The effects of Omga-3PUFA on MNU-induced colorectal cancer in rats were analyzed by in vivo experiments. The viability, apoptosis, colony formation and invasion of CRC cells treated with Omga-3PUFA were detected by CCK8, flow cytometry, clone formation assay and transwell invasion assay. The expression of apoptosis-related proteins in CRC cells treated with Omga-3PUFA was detected by Western blotting. Finally, after adding PI3K activator, the viability, apoptosis and protein expression of CRC cells treated with Omga-3PUFA were detected by CCK8, flow cytometry and Western blotting. Results: Our results showed that Omega-3PUFA attenuated MNU-induced CRC in rats and inhibited AKT/Bcl-2 signaling in rats. In addition, Omega-3PUFA inhibited CRC cell proliferation and induces CRC cell apoptosis. Moreover, Omega-3PUFA inhibited CRC cell colony formation and invasion, and inhibited PI3K/AKT/Bcl-2 signaling in CRC cells. Furthermore, The effects of Omega-3PUFA on cell proliferation and apoptosis were inhibited by blocking PI3K/AKT signaling. Conclusion: Omega-3PUFA can attenuate MNU-induced colorectal cancer in rats by blocking PI3K/AKT/Bcl-2 signaling, which suggests that Omega-3PUFA may be a potent agent for CRC treatment.

show abstract

“…The PI3K/AKT signaling pathway is a downstream signaling pathway of epidermal growth factor receptor, which is overexpressed in a variety of malignant tumors, such as breast [ 32 ], ovarian [ 33 ], lung [ 34 ], and liver cancers [ 35 ]. The activation rate of this pathway is at a high level in BC, and its abnormal activation can induce the proliferation and differentiation of tumor cells and can affect cell cycle [ 36 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

Hypermethylation of DLG3 Promoter Upregulates RAC1 and Activates the PI3K/AKT Signaling Pathway to Promote Breast Cancer Progression

Liu

Zhang

et al. 2021

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Objective. This investigation aimed to figure out the relation between discs large homolog 3 (DLG3) expression and the progression and prognosis of breast cancer (BC). Methods. qRT-PCR was utilized for confirming DLG3 expression and RAC1 mRNA expression in BC tissues and cells. Subsequently, after overexpression or interference of DLG3, the changes of the biological activities of BC cells, including cell proliferation, migration, invasion, and apoptosis, were detected through CCK-8, colony formation assay, wound healing assay, transwell assay, and flow cytometry, respectively. Furthermore, western blotting was utilized to measure the protein expression of DLG3 and RAC1, as well as related proteins of epithelial-mesenchymal transition (EMT) and the PI3K/AKT signaling pathway. Results. At both cellular and tissue level in BC, DLG3 was downregulated and methylation level was upregulated; RAC1 showed an opposite change and was of a negative correlation with DLG3. In MCF-7 and HCC1937, we found that the upregulation of DLG3 could inhibit RAC1 expression as well as cell proliferation, invasion, migration, and EMT, while promoting apoptosis. Also, DLG3 inhibited the activation of the P13K/AKT pathway. Conclusion. Hypermethylation of DLG3 promoter upregulates RAC1 and activates the PI3K/AKT pathway, thus promoting BC progression. This conclusion provides ideas and experimental basis for improving and treating BC patients.

show abstract

A combination of Citrus reticulata peel and black tea inhibits migration and invasion of liver cancer via PI3K/AKT and MMPs signaling pathway

Cited by 17 publications

References 57 publications

Ethanolic Extract of Citrus reticulata Peel Inhibits the Migration of WiDr Colon Cancer Cells

Ethanolic Extract of Citrus reticulata Peel Inhibits the Migration of WiDr Colon Cancer Cells

<p>Omega-3PUFA Attenuates MNU-Induced Colorectal Cancer in Rats by Blocking PI3K/AKT/Bcl-2 Signaling</p>

Hypermethylation of DLG3 Promoter Upregulates RAC1 and Activates the PI3K/AKT Signaling Pathway to Promote Breast Cancer Progression

Contact Info

Product

Resources

About