<p>Omega-3PUFA Attenuates MNU-Induced Colorectal Cancer in Rats by Blocking PI3K/AKT/Bcl-2 Signaling</p>

Abstract

Background: Omega 3 polyunsaturated fatty acid (Omega-3PUFA) is one of the essential nutrients for human body involved in intracellular metabolic regulation and cell signaling. Previous studies have shown that Omega-3PUFA is involved in the pathogenesis of digestive system tumors, including colorectal cancer (CRC), however, the effects of Omega-3PUFA on CRC has not been fully elucidated. In the current study, we evaluated whether Omega-3PUFA can alleviate N-methyl-N-nitrosourea(MNU) induced CRC in a rat model and illustrated the potential mechanism. Methods: The effects of Omga-3PUFA on MNU-induced colorectal cancer in rats were analyzed by in vivo experiments. The viability, apoptosis, colony formation and invasion of CRC cells treated with Omga-3PUFA were detected by CCK8, flow cytometry, clone formation assay and transwell invasion assay. The expression of apoptosis-related proteins in CRC cells treated with Omga-3PUFA was detected by Western blotting. Finally, after adding PI3K activator, the viability, apoptosis and protein expression of CRC cells treated with Omga-3PUFA were detected by CCK8, flow cytometry and Western blotting. Results: Our results showed that Omega-3PUFA attenuated MNU-induced CRC in rats and inhibited AKT/Bcl-2 signaling in rats. In addition, Omega-3PUFA inhibited CRC cell proliferation and induces CRC cell apoptosis. Moreover, Omega-3PUFA inhibited CRC cell colony formation and invasion, and inhibited PI3K/AKT/Bcl-2 signaling in CRC cells. Furthermore, The effects of Omega-3PUFA on cell proliferation and apoptosis were inhibited by blocking PI3K/AKT signaling. Conclusion: Omega-3PUFA can attenuate MNU-induced colorectal cancer in rats by blocking PI3K/AKT/Bcl-2 signaling, which suggests that Omega-3PUFA may be a potent agent for CRC treatment.

17β-Estradiol promotes angiogenesis of bone marrow mesenchymal stem cells by upregulating the PI3K-Akt signaling pathway

“…EGFR can activate the PI3K-Akt signaling pathway and promote the proliferation and migration of seminoma cells [41] . Blocking the PI3K-Akt-BCL-2 signaling pathway can reduce the occurrence and development of colorectal cancer in SD rats [42] .…”

Periplocymarin Induced Colorectal Cancer Cells Apoptosis Via Impairing PI3K/AKT Pathway

“…Activated PI3K/AKT signaling pathway has been suggested favorable for cells survival through inhibiting apoptosis and impair cell cycle arrest by targeting downstream genes, including Bcl-2 family, caspase family, survivin, p21, and cyclin D (39)(40)(41)(42)(43). Inhibition of PI3K/AKT signaling pathway has been found effective in inhibition of tumor cells of different tissues (44)(45)(46). IRS1, upstream of PI3K and major substrate of insulin, insulin-like growth factors and cytokine signaling, plays an important role in mediating apoptosis, cell differentiation, and cell transformation (47).…”

The Preparation of Gen-NH2-MCM-41@SA Nanoparticles and Their Anti-Rotavirus Effects

“…After the Kunming mice were 4 days old, 200 µL of DMEM or RV-SA at a virus titer of 10 −4.64 TCID50/mL was administered to them via oral gavage. After gavage, the neonatal mice were placed back with their mothers and fed normal breastmilk for three consecutive days [ 40 , 41 ].…”

“…MCM-41 (Mobil Composition of Matter No. 41) is a mesoporous material with a hierarchical structure(as shown in Figure 2)from a family of silicate and aluminosilicate solids that were first developed by researchers at Mobil Oil Corporation and can be used as catalysts or catalyst supports [8].…”