A colorimetric and fluorescent gold nanoparticle-based dual-mode aptasensor for parvalbumin detection

Li, Han; Li, Huan; Zhou, Jinru; Qi, Qinqin; Fu, Linglin

doi:10.1016/j.microc.2020.105413

Cited by 25 publications

(5 citation statements)

References 24 publications

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“…Considering the fast detection of colorimetric sensing and high sensitivity detection of fluorometric sensing, a dual-mode aptasensor based on both colorimetric and fluorescent readouts was developed, with the potential advantages of high sensitivity, naked-eye monitoring, and easy and fast operation. Wang et al [ 57 ] proposed a dual-mode aptasensor for parvalbumin (PV) detection (see Figure 4 D). In this strategy, aptamer-modified AuNPs (AuNP-APT) were hybridized with short complementary sequence 1 (CS1)-conjugated AuNPs (AuNP-CS1) and complementary sequence 2 (CS2)-conjugated fluorescent dye (FAM-CS2).…”

Section: Strategies For Nanoparticle-based Aptasensorsmentioning

confidence: 99%

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Recent Advances in Biomolecular Detection Based on Aptamers and Nanoparticles

Ouyang

Chen

et al. 2023

Biosensors

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The fast, accurate detection of biomolecules, ranging from nucleic acids and small molecules to proteins and cellular secretions, plays an essential role in various biomedical applications. These include disease diagnostics and prognostics, environmental monitoring, public health, and food safety. Aptamer recognition (DNA or RNA) has gained extensive attention for biomolecular detection due to its high selectivity, affinity, reproducibility, and robustness. Concurrently, biosensing with nanoparticles has been widely used for its high carrier capacity, stability and feasibility of incorporating optical and catalytic activity, and enhanced diffusivity. Biosensors based on aptamers and nanoparticles utilize the combination of their advantages and have become a promising technology for detecting of a wide variety of biomolecules with high sensitivity, reliability, specificity, and detection speed. Via various sensing mechanisms, target biomolecules have been quantified in terms of optical (e.g., colorimetric and fluorometric), magnetic, and electrical signals. In this review, we summarize the recent advances in and compare different aptamer–nanoparticle-based biosensors by nanoparticle types and detection mechanisms. We also share our views on the highlights and challenges of the different nanoparticle-aptamer-based biosensors.

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Section: Strategies For Nanoparticle-based Aptasensorsmentioning

confidence: 99%

“…Copyright 2021 Microchimica Acta. ( D ) Dual-mode aptasensor for PV detection based on state transition of AuNPs and fluorescence variation of FAM-CS2, reproduced with permission from [ 57 ]. Copyright 2020 Microchemical Journal.…”

Section: Strategies For Nanoparticle-based Aptasensorsmentioning

confidence: 99%

Recent Advances in Biomolecular Detection Based on Aptamers and Nanoparticles

Ouyang

Chen

et al. 2023

Biosensors

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“…1 ppm), which is comparable with that of commercially available LFIAs for α s -casein. Wang et al developed a dual mode aptasensor for the detection of parvalbumin with both colorimetric and fluorescent signal readouts [ 46 ]. The aptasensor was obtained by hybridizing a gold nanoparticle-modified aptamer (AuNP-APT) with complementary short-chain oligonucleotides modified either with gold nanoparticles (AuNP-CS1) or a fluorescent dye (FAM-CS2).…”

Section: Luminescence-based Aptasensors For Food Allergensmentioning

confidence: 99%

Luminescent Aptamer-Based Bioassays for Sensitive Detection of Food Allergens

et al. 2022

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The presence of hidden allergens in food products, often due to unintended contamination along the food supply chain (production, transformation, processing, and transport), has raised the urgent need for rapid and reliable analytical methods for detecting trace levels of such species in food products. Indeed, food allergens represent a high-risk factor for allergic subjects due to potentially life-threatening adverse reactions. Portable biosensors based on immunoassays have already been developed as rapid, sensitive, selective, and low-cost analytical platforms that can replace analyses with traditional bench-top instrumentation. Recently, aptamers have attracted great interest as alternative biorecognition molecules for bioassays, since they can bind a variety of targets with high specificity and selectivity, and they enable the development of assays exploiting a variety of transduction and detection technologies. In particular, aptasensors based on luminescence detection have been proposed, taking advantage of the development of ultrasensitive tracers and enhancers. This review aims to summarize and discuss recent efforts in the field of food allergen analysis using aptamer-based bioassays with luminescence detection.

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“…Dual-mode nanosensors usually use colorimetric and fluorescent reporters to achieve convenient visual inspection and highly sensitive fluorescent detection [160,161]. Wang et al developed a dual-mode aptamer-based fluorescent sensor for the detection of PV, the major allergen of fish (Figure 3B) [159]. In Figure 3C(a), aptamer towards PV was obtained by in vitro screening of random ssDNA library containing a 40-mer randomized region using the triple-mode GO-SELEX.…”

Section: Detection Of Animal Food Allergensmentioning

confidence: 99%

Aptamer-Based Fluorescent Biosensor for the Rapid and Sensitive Detection of Allergens in Food Matrices

Hong

Pan

Xie

et al. 2021

Foods

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Food allergies have seriously affected the life quality of some people and even endangered their lives. At present, there is still no effective cure for food allergies. Avoiding the intake of allergenic food is still the most effective way to prevent allergic diseases. Therefore, it is necessary to develop rapid, accurate, sensitive, and reliable analysis methods to detect food allergens from different sources. Aptamers are oligonucleotide sequences that can bind to a variety of targets with high specificity and selectivity, and they are often combined with different transduction technologies, thereby constructing various types of aptamer sensors. In recent years, with the development of technology and the application of new materials, the sensitivity, portability, and cost of fluorescence sensing technology have been greatly improved. Therefore, aptamer-based fluorescence sensing technology has been widely developed and applied in the specific recognition of food allergens. In this paper, the classification of major allergens and their characteristics in animal and plant foods were comprehensively reviewed, and the preparation principles and practical applications of aptamer-based fluorescence biosensors are summarized. In addition, we hope that this article can provide some strategies for the rapid and sensitive detection of allergens in food matrices.

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A colorimetric and fluorescent gold nanoparticle-based dual-mode aptasensor for parvalbumin detection

Cited by 25 publications

References 24 publications

Recent Advances in Biomolecular Detection Based on Aptamers and Nanoparticles

Recent Advances in Biomolecular Detection Based on Aptamers and Nanoparticles

Luminescent Aptamer-Based Bioassays for Sensitive Detection of Food Allergens

Aptamer-Based Fluorescent Biosensor for the Rapid and Sensitive Detection of Allergens in Food Matrices

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