A Color Test for Dehydroisoandrosterone and Closely Related Steroids, of Use in the Diagnosis of Adrenocortical Tumors

Allen, Willard M.; Hayward, S. J.; Pinto, Alexandre

doi:10.1210/jcem-10-1-54

Cited by 114 publications

(15 citation statements)

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“…Authentic steroid markers were run alongside and were detected by UV light (254 nm wavelength) for 4-en-3-oxosteroids or with iodine vapour and Allen reagent (Allen, Hayward & Pinto, 1950). This method success¬ fully resolved 5oc-androst-16-en-3-one, 5oc-androst-16-en-3a-ol and 4,16-androstadien-3-one from each other and from a mixture of 5a-androst-16-en-3ß-ol plus 5,16-androstadien-3ß-ol.…”

Section: Separation Of Metabolitesmentioning

confidence: 99%

Comparative rates of formation, in vivo, of 16-androstenes, testosterone and androstenedione in boar testis

Hurden¹,

Gower²,

Harrison³

1984

Journal of Endocrinology

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Three mature Large White boars were anaesthetized and received [7(n)-3H]pregnenolone by continuous infusion into right and left spermatic arteries for up to 180 min. Spermatic venous blood flow was measured by separate timed collections of completely diverted outflow from each testis and blood not sampled was returned to the peripheral circulation. The total radioactivity in plasma from each testis increased markedly during the first 60 min of infusion to reach a plateau from 80 to 180 min. Radiolabelling of 5 alpha-androst-16-en-3-one, 5 alpha-androst-16-en-3 beta-ol and -3 alpha-ol showed similar patterns with ratios of mean radioactivity of 5:3:1 respectively between 80 and 180 min. In comparison, the amounts of tritiated 4,16-androstadien-3-one formed were very small. The radiolabelling of testosterone and 4-androstenedione occurred more rapidly than that of the 16-androstenes and reached maxima by 30 min. However the amounts were only one-fifth (testosterone) and one-tenth (4-androstenedione) those of the combined quantities of tritiated 16-androstenes. Addition of human chorionic gonadotrophin (hCG) to the infusate to one testis in each animal (so that 5000 i.u. hCG were delivered in 15-20 min) produced no change in the outputs of radiolabelled steroids although radioimmunoassay of spermatic venous plasma in samples from the third experiment showed a transient increase in the concentration of 4-androstene-3,17-dione during the hCG infusion. It is suggested the lack of response to hCG could be produced by saturation and down regulation of binding sites by the very high local concentrations of hCG.

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Section: Separation Of Metabolitesmentioning

confidence: 99%

Comparative rates of formation, in vivo, of 16-androstenes, testosterone and androstenedione in boar testis

Hurden¹,

Gower²,

Harrison³

1984

Journal of Endocrinology

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show abstract

“…It is of interest that the dehydroisoandrosterone test (7), which is generally considered to be more likely to yield a positive result in adrenal tumors, was of no help in the differential diagnosis in this case. In fact, the negative result was misleading.…”

mentioning

confidence: 92%

Differential Diagnosis of Virilism

Segaloff

Gordon

Horwitt

et al. 1955

The Journal of Clinical Endocrinology & Metabolism

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“…The technic for total neutral 17-ketosteroids was a modification of that described by HoltorfT and Koch (1). Dehydroisoandrosterone was determined by the method of Allen, Hayward, and Pinto (2). In the 2 patients with increased neutral 17-ketosteroid excretions, the "phenolic fraction" obtained by extraction with IN KOH was separated from the carbon tetrachloride extract and was used for bioassay of estrogens.…”

Section: Introductionmentioning

confidence: 99%

Increased Urinary Estrogen Excretion Associated With Adrenal Tumors: Report of Four Cases*

Dohan

Rose

Eiman

et al. 1953

The Journal of Clinical Endocrinology & Metabolism

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A Color Test for Dehydroisoandrosterone and Closely Related Steroids, of Use in the Diagnosis of Adrenocortical Tumors

Cited by 114 publications

References 0 publications

Comparative rates of formation, in vivo, of 16-androstenes, testosterone and androstenedione in boar testis

Comparative rates of formation, in vivo, of 16-androstenes, testosterone and androstenedione in boar testis

Differential Diagnosis of Virilism

Increased Urinary Estrogen Excretion Associated With Adrenal Tumors: Report of Four Cases*

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