2007
DOI: 10.1111/j.1538-7836.2007.02684.x
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A collaborative study to establish the 1st International Standard for factor XIII plasma

Abstract: JapanTo cite this article: Raut S, Merton RE, Rigsby P, Muszbek L, Seitz R, Arië ns RAS, Barrowcliffe TW, Ichinose A, on behalf of the ISTH/SSC Factor XIII Subcommittee and the Factor XIII Standardization Working Party. A collaborative study to establish the first International Standard for factor XIII plasma. J Thromb Haemost 2007; 5: 1923-9. Summary. Background: An international collaborative study, involving 23 laboratories, was carried out, under the auspices of the FXIII Standardization Working Party (… Show more

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Cited by 17 publications
(16 citation statements)
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“…Human fibronectin (5 μg per well) was used to coat 96‐well plates (CoStar; Corning, Lowell, MA, USA). The FXIII standard was pooled normal plasma, standardized against the international standard [32], preactivated with 1 U mL −1 bovine thrombin at 37 °C for 5 min. Residual thrombin was neutralized by hirudin (2 μg mL −1 ), and samples were diluted in 0.1 m Tris (pH 7.4) and 1 m m dithiothreitol (DTT), to construct a standard curve.…”
Section: Methodsmentioning
confidence: 99%
“…Human fibronectin (5 μg per well) was used to coat 96‐well plates (CoStar; Corning, Lowell, MA, USA). The FXIII standard was pooled normal plasma, standardized against the international standard [32], preactivated with 1 U mL −1 bovine thrombin at 37 °C for 5 min. Residual thrombin was neutralized by hirudin (2 μg mL −1 ), and samples were diluted in 0.1 m Tris (pH 7.4) and 1 m m dithiothreitol (DTT), to construct a standard curve.…”
Section: Methodsmentioning
confidence: 99%
“…The WHO First International Standard FXIII plasma (National Institute for Biological Standards and Control, Hertfordshire, UK) was used. 42 The reaction was performed in 100 mM TrisHCl [pH 7.4], 1 mM dithiothreitol, 1% BSA, 0.5 mM 5-(biotinamido) pentylamine (Pierce Thermo Fisher Scientific, Rockford, IL), and 5 mM CaCl 2 . Plates were washed with 0.01% Triton-X-100 in 100 mM Tris-HCl [pH 7.4] before adding para-nitrophenyl phosphate substrate (Sigma-Aldrich).…”
Section: Preparation Of Platelet Fractionsmentioning
confidence: 99%
“…Most recently, based on the standardization activity of the Factor XIII Standardization Working Party of the ISTH SSC [8], a World Health Organization reference plasma with assigned pFXIII activity and FXIII‐A 2 B 2 antigen values in International Units (1st International Standard for Factor XIII, Plasma [IS]) was established. The IS is available from the National Institute of Biological Standards and Control (Potters Bar, UK).…”
Section: Laboratory Diagnosismentioning
confidence: 99%