A Collaborative Study: High-Pressure Liquid Chromatographic Determination of Carbadox and Pyrantel Tartrate in Animal Feeds

Abstract

Carbadox (CBX), an antibacterial agent, and pyrantel tartrate (PT), an anthelmintic, are formulated either separately or together in swine feeds. The official Association of Official Analytical Chemists (AOAC) spectrophotometric methods for both drugs are long, nonspecific, and require standard addition techniques. Results by this technique are positively biased. A simple, direct, specific, high-pressure liquid chromatographic (HPLC) method to determine either one or both drugs simultaneously with apparent accuracy and precision is developed. Drugs are released from feed matrices by water, extracted with dimethylformamide (DMF), cleaned up on alumina, and quantitated by direct comparison to standards using a Whatman Partisil 10 ODS-3 column and a mobile solvent containing 23.5 +/- 1.5% DMF in phosphate buffer (pH 2.0). Fourteen laboratories participated in a collaborative study of this method for determination of CBX and PT in animal feeds.

High-Performance Liquid Chromatographic Determination of Carbadox, Olaquindox, Furazolidone, Nitrofurazone, and Nitrovin in Feed

“…Most of the previously published methods for the determinations of OLQ, CBX, NF, FZ, and NTV in feed are concerned only with one (single) drug in feed (1-4, 8, 9, 11, 14, 16-19), and a few experiments were conducted to simultaneously determine two (5,10,12,13,20,22), three (15), or four drugs (21). As for this work, there were no reports on the simultaneous determination of residual drugs of OLQ, CBX, NF, FZ, and NTV in feed by high-performance liquid chromatography (HPLC).…”