A CMOS Multi-Sensor Array for High Accuracy On-Chip Bacterial Growth Monitoring

Duan, Mingzheng; Zhong, Xiaopeng; Gao, Feng; Bermak, Amine; Lee, Yi-Kuen

doi:10.1109/iscas45731.2020.9180794

Cited by 3 publications

(3 citation statements)

References 14 publications

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“…The temperature signal is buffered before digitization by a p-channel source follower to maintain its linearity. The temperature signal is simulated to have a sensitivity of 2 mV/ • C and excellent linearity (R 2 =1) within a sensing range of 20 • C to 60 • C [28].…”

Section: Heating Regulationmentioning

confidence: 99%

“…Although there have been reported thermal regulation systems for various applications, such as DNA polymer chain reaction (PCR) and cell culturing [24]- [27], to the best of our knowledge, this is the first attempt to combine optical sensor, temperature regulation, and on-chip readout electronics, providing an integrated analysis on a highly integrated CMOS chip for real-time bacterial growth monitoring and its response to antibiotics. The proposed LoC, an extension of [28], as shown in Fig. 1, uses CMOS photodiodes to detect optical signal correlating with bacterial concentration.…”

Section: Introductionmentioning

confidence: 99%

“…A tube coated with parylene-c is mounted and glued above the packaged chip for sample culturing. Following brief introduction and simulation results of this work in the conference paper [28], this paper presents detailed circuit implementation, characterization results of individual sensor nodes, analog-to-digital converter (ADC), temperature regulation, and on-chip biologic test, as well as discussions of the proposed LoC platform.…”

Section: Introductionmentioning

confidence: 99%

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A Highly Integrated Lab-on-a-CMOS Platform for Real-Time Monitoring of E. Coli Growth Kinetics

Duan,

Zhong,

Wang

et al. 2024

IEEE Trans. Biomed. Circuits Syst.

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Existing miniaturized and cost-effective solutions for bacterial growth monitoring usually require offline incubators with constant temperature to culture the bio-samples prior to measurement. Such a separated sample preparation and detection scheme requires extensive human intervention, risks contamination, and suffers from poor temporal resolution. To achieve integrated sample preparation and real-time bacterial growth monitoring, this paper presents a lab-on-a-CMOS platform incorporates an optical sensor array, temperature sensor array, micro-heaters, and readout circuits. Escherichia coli's (E. coli) optimum growth temperature of 37 • C is achieved through a heat regulation system consisting of two microheaters and an on-chip temperature sensor array. A photodiode array with an in-pixel capacitive trans-impedance amplifier to reduce inter-pixel cross-coupling is designed to extract the optical information during bacterial growth. To balance the footprint, power consumption, and quantization speed, a 10bit column successive-approximation register (SAR)/single-slope (SS) dualmode analog-to-digital converter (ADC) is designed to digitize the temperature and optical signals. Fabricated in a standard 0.18 um CMOS process, the proposed platform can regulate the sample temperature to 37 +/-0.2/0.3 • C within 32 mins. Enabled by an on-chip heat regulation system and photodetectors, the prototype demonstrates a real-time monitoring of bacterial growth kinetics and antibiotic responses. Minute-level temporal resolution is achieved as this proposed platform is free of extensive and time-consuming human intervention. The proposed platform can be viably used in contamination sensitive applications such as antibiotic tests, stem cell cultures, and organ-on-chips.

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Section: Heating Regulationmentioning

confidence: 99%