A CMOS-based on-chip neural interface device equipped with integrated LED array for optogenetics

Tokuda, Takashi; Miyatani, Tomoaki; Maezawa, Yasuyo; Kobayashi, Takuma; Noda, Toshihiko; Sasagawa, Kiyotaka

doi:10.1109/embc.2012.6347152

Cited by 4 publications

(4 citation statements)

References 11 publications

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“…To truly realize an implantable technique, a more miniaturized design is required. Fortunately, with the rapid development of the electronic engineering technology, more and more miniature electronic components, especially the ultra‐micro‐sized version of the surface mount LEDs and Photodiode, appeared and was widely applied in the field of biomedical imaging . Based on these new techniques, a miniature probe more suitable for implantation in animal and human brain can be achieved.…”

Section: Discussionmentioning

confidence: 99%

“…Fortunately, with the rapid development of the electronic engineering technology, more and more miniature electronic components, especially the ultra-micro-sized version of the surface mount LEDs and Photodiode, appeared and was widely applied in the field of biomedical imaging. 36,37 Based on these new techniques, a miniature probe more suitable for implantation in animal and human brain can be achieved. The inflexible configuration of the interface is also one of the limitations of this design, which may affect the accuracy of ESL imaging.…”

Section: Discussionmentioning

confidence: 99%

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In vivo imaging of epileptic foci in rats using a miniature probe integrating diffuse optical tomography and electroencephalographic source localization

et al. 2014

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SUMMARY Objective The goal of this work is to establish a new dual-modal brain mapping technique based on diffuse optical tomography (DOT) and electroencephalographic source localization (ESL) that can chronically/intracranially record optical/EEG data to precisely map seizures and localize the seizure onset zone and associated epileptic brain network. Methods The dual-modal imaging system was employed to image seizures in an experimental acute bicuculline methiodide rat model of focal epilepsy. Depth information derived from DOT was used as constraint in ESL to enhance the image reconstruction. Groups of animals were compared based on localization of seizure foci, either at different positions or at different depths. Results This novel imaging technique successfully localized the seizure onset zone in rat induced by bicuculline methiodide injected at a depth of 1mm, 2mm and 3mm, respectively. The results demonstrated that the incorporation of the depth information from DOT into the ESL image reconstruction resulted in more accurate and reliable ESL images. Although the ESL images showed a horizontal shift of the source localization, the DOT identified the seizure focus accurately. In one case, when the BMI was injected at a site outside the field of view (FOV) of the DOT/ESL interface, ESL gives false positive detection of the focus while DOT shows negative detection. Significance This study represents the first to identify seizure onset zone using implantable DOT. In addition, the combination of DOT/ESL has never been documented in neuroscience and epilepsy imaging. This technology will enable us to precisely measure the neural activity and hemodynamic response at exactly the same tissue site and at both cortical and sub cortical levels.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

In vivo imaging of epileptic foci in rats using a miniature probe integrating diffuse optical tomography and electroencephalographic source localization

et al. 2014

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“…We have proposed and demonstrated a CMOS-based optoelectronic neural interface device with an integrated LED array for on-chip stimulation [6,7]. Figure 1 shows the concept of the CMOS-based neural interface device.…”

Section: Introductionmentioning

confidence: 98%

An in vitro demonstration of CMOS-based optoelectronic neural interface device for optogenetics

Tokuda

Nakajima

Maezawa

et al. 2013

2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)

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A CMOS-based neural interface device equipped with an integrated micro light source array for optogenetics was fabricated and demonstrated. A GaInN LED array formed on sapphire substrate was successfully assembled with a multifunctional CMOS image sensor that is capable of on-chip current injection. We demonstrated a functionality of light stimulation onto ChR2-expressed cells in an in vitro experiment. A ChR2-expressed cell were successfully stimulated with the light emitted from the fabricated device.

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“…Such a technique would be useful for functional brain analysis and could be used to control perception, behavior, and emotion in a freely moving animal. As such, optical neural interface devices have been investigated heretofore 26 27 28 29 30 31 . In the present study, an implantable device incorporating optogenetics and Ca 2+ imaging and its control system was newly developed.…”

mentioning

confidence: 99%

“Optical communication with brain cells by means of an implanted duplex micro-device with optogenetics and Ca2+ fluoroimaging”

Kobayashi

Haruta

Sasagawa

et al. 2016

Sci Rep

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To better understand the brain function based on neural activity, a minimally invasive analysis technology in a freely moving animal is necessary. Such technology would provide new knowledge in neuroscience and contribute to regenerative medical techniques and prosthetics care. An application that combines optogenetics for voluntarily stimulating nerves, imaging to visualize neural activity, and a wearable micro-instrument for implantation into the brain could meet the abovementioned demand. To this end, a micro-device that can be applied to the brain less invasively and a system for controlling the device has been newly developed in this study. Since the novel implantable device has dual LEDs and a CMOS image sensor, photostimulation and fluorescence imaging can be performed simultaneously. The device enables bidirectional communication with the brain by means of light. In the present study, the device was evaluated in an in vitro experiment using a new on-chip 3D neuroculture with an extracellular matrix gel and an in vivo experiment involving regenerative medical transplantation and gene delivery to the brain by using both photosensitive channel and fluorescent Ca2+ indicator. The device succeeded in activating cells locally by selective photostimulation, and the physiological Ca2+ dynamics of neural cells were visualized simultaneously by fluorescence imaging.

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A CMOS-based on-chip neural interface device equipped with integrated LED array for optogenetics

Cited by 4 publications

References 11 publications

In vivo imaging of epileptic foci in rats using a miniature probe integrating diffuse optical tomography and electroencephalographic source localization

In vivo imaging of epileptic foci in rats using a miniature probe integrating diffuse optical tomography and electroencephalographic source localization

An in vitro demonstration of CMOS-based optoelectronic neural interface device for optogenetics

“Optical communication with brain cells by means of an implanted duplex micro-device with optogenetics and Ca2+ fluoroimaging”

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