An in vitro demonstration of CMOS-based optoelectronic neural interface device for optogenetics

Tokuda, Takashi; Nakajima, Shun; Maezawa, Yasuyo; Noda, Toshihiko; Sasagawa, Kiyotaka; Ishikawa, Yasuyuki; Shiosaka, Sadao

doi:10.1109/embc.2013.6609621

Cited by 4 publications

(3 citation statements)

References 9 publications

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“…The CMOS image sensor is designed and fabricated using the standard CMOS process (Austria Microsystems, 0.35 µm 2-poly 4-metal CMOS). 15,16) The CMOS image sensor contains a pixel array that detects light intensity using photodiodes. The pixel size of the sensor is 7.5 × 7.5 µm 2 ; the sensor contains a 260 × 244 pixel array, Xand Y-scanners, and column amplification circuits.…”

Section: Implantable Optogenetic Devices and Systemsmentioning

confidence: 99%

Implantable optogenetic device with CMOS IC technology for simultaneous optical measurement and stimulation

Haruta

Kamiyama

Nakajima

et al. 2017

Jpn. J. Appl. Phys.

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In this study, we have developed an implantable optogenetic device that can measure and stimulate neurons by an optical method based on CMOS IC technology. The device consist of a blue LED array for optically patterned stimulation, a CMOS image sensor for acquiring brain surface image, and eight green LEDs surrounding the CMOS image sensor for illumination. The blue LED array is placed on the CMOS image sensor. We implanted the device in the brain of a genetically modified mouse and successfully demonstrated the stimulation of neurons optically and simultaneously acquire intrinsic optical images of the brain surface using the image sensor. The integrated device can be used for simultaneously measuring and controlling neuronal activities in a living animal, which is important for the artificial control of brain functions.Jpn. J. Appl. Phys. 56, 057001 (2017) M. Haruta et al. 057001-3

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Section: Implantable Optogenetic Devices and Systemsmentioning

confidence: 99%

Implantable optogenetic device with CMOS IC technology for simultaneous optical measurement and stimulation

Haruta

Kamiyama

Nakajima

et al. 2017

Jpn. J. Appl. Phys.

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“…Optogenetics comprises a set of techniques that integrate the opsin genes into specific types of neurons to selectively probe the neural circuits [ 1 , 2 ] and has currently been introduced into a growing number of neuroscience researches [ 3 – 6 ] and neural engineering systems such as the brain-machine interfaces [ 7 , 8 ]. The optogenetic technique enables either excitation or inhibition of selected neural populations under the delivery of light at specific wavelengths [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Optogenetic Activation of the Excitatory Neurons Expressing CaMKIIαin the Ventral Tegmental Area Upregulates the Locomotor Activity of Free Behaving Rats

Guo

Chen

Zhang

et al. 2014

BioMed Research International

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The ventral tegmental area (VTA) plays an important role in motivation and motor activity of mammals. Previous studies have reported that electrical stimulations of the VTA's neuronal projections were able to upregulate the locomotor activity of behaving rats. However, which types of neurons in the VTA that take part in the activation remain elusive. In this paper we employed optogenetic technique to selectively activate the excitatory neurons expressing CaMKIIα in the VTA region and induced a higher locomotor activity for free behaving rats. Further behavioral studies indicated that reward learning mediated in the enhancement of the rat locomotor activity. Finally the immunohistochemistry studies explored that the excitatory neurons under the optogenetic activation in VTA were partly dopaminergic that may participate as a vital role in the optogenetic activation of the locomotor activity. In total, our study provided an optogenetic approach to selectively upregulate the locomotor activity of free behaving rats, thus facilitating both neuroscience researches and neural engineering such as animal robotics in the future.

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“…Such a technique would be useful for functional brain analysis and could be used to control perception, behavior, and emotion in a freely moving animal. As such, optical neural interface devices have been investigated heretofore 26 27 28 29 30 31 . In the present study, an implantable device incorporating optogenetics and Ca 2+ imaging and its control system was newly developed.…”

mentioning

confidence: 99%

“Optical communication with brain cells by means of an implanted duplex micro-device with optogenetics and Ca2+ fluoroimaging”

Kobayashi

Haruta

Sasagawa

et al. 2016

Sci Rep

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To better understand the brain function based on neural activity, a minimally invasive analysis technology in a freely moving animal is necessary. Such technology would provide new knowledge in neuroscience and contribute to regenerative medical techniques and prosthetics care. An application that combines optogenetics for voluntarily stimulating nerves, imaging to visualize neural activity, and a wearable micro-instrument for implantation into the brain could meet the abovementioned demand. To this end, a micro-device that can be applied to the brain less invasively and a system for controlling the device has been newly developed in this study. Since the novel implantable device has dual LEDs and a CMOS image sensor, photostimulation and fluorescence imaging can be performed simultaneously. The device enables bidirectional communication with the brain by means of light. In the present study, the device was evaluated in an in vitro experiment using a new on-chip 3D neuroculture with an extracellular matrix gel and an in vivo experiment involving regenerative medical transplantation and gene delivery to the brain by using both photosensitive channel and fluorescent Ca2+ indicator. The device succeeded in activating cells locally by selective photostimulation, and the physiological Ca2+ dynamics of neural cells were visualized simultaneously by fluorescence imaging.

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An in vitro demonstration of CMOS-based optoelectronic neural interface device for optogenetics

Cited by 4 publications

References 9 publications

Implantable optogenetic device with CMOS IC technology for simultaneous optical measurement and stimulation

Implantable optogenetic device with CMOS IC technology for simultaneous optical measurement and stimulation

Optogenetic Activation of the Excitatory Neurons Expressing CaMKIIαin the Ventral Tegmental Area Upregulates the Locomotor Activity of Free Behaving Rats

“Optical communication with brain cells by means of an implanted duplex micro-device with optogenetics and Ca2+ fluoroimaging”

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