A closed system for the clinical banking of umbilical cord blood

Adami, Valentina; Malangone, W.; Falasca, E; Marini, L. D.; Risso, Angela; Crini, S.; Toniutti, E.; Ferraro, Elisa; Frate, G. Del; Pittino, Marco; Biffoni, Franco; Rinaldi, Cristina; Degrassi, A.

doi:10.1016/j.bcmd.2005.07.008

Cited by 8 publications

(4 citation statements)

References 43 publications

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“…The number of total nucleated cells is being utilized to determine the suitability of a CB unit prepared for transplantation 3,19 . In addition, the number of CD34+ cells may be a good indicator of quality 13,19‐22 . The methods being used with CB stem and progenitor cells are similar to those utilized with peripheral blood and marrow preparations.…”

Section: Discussionmentioning

confidence: 99%

Assessment of cord blood hematopoietic cell parameters before and after cryopreservation

Kurtz

Seetharaman²,

Greco³

et al. 2007

Transfusion

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Section: Discussionmentioning

confidence: 99%

Assessment of cord blood hematopoietic cell parameters before and after cryopreservation

Kurtz

Seetharaman²,

Greco³

et al. 2007

Transfusion

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“…In the near future, one can expect to see advances in both basic science investigations on neuronal differentiation of umbilical cord‐derived stem cells in culture and in vivo and attempts to apply and optimize its therapeutic efficacies in clinical trials of neurotrauma and neural diseases. Methods of banking and preservation of umbilical cord blood cells for hematopoietic therapies are reasonably well established (see, e.g., Adami et al, 2005; Meyer et al, 2006). However, optimized protocols, particularly for the clonal expansion of stem cells in umbilical cord blood/stroma, and the subsequent cryopreservation have to be carefully developed.…”

Section: The Promise Of Human Umbilical Cord‐derived Stem Cells In Thmentioning

confidence: 99%

Neural differentiation and potential use of stem cells from the human umbilical cord for central nervous system transplantation therapy

Low

Liou

Tang

2008

J of Neuroscience Research

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The human umbilical cord is a rich source of autologous stem and progenitor cells. Interestingly, subpopulations of these, particularly mesenchymal-like cells from both cord blood and the cord stroma, exhibited a potential to be differentiated into neuron-like cells in culture. Umbilical cord blood stem cells have demonstrated efficacy in reducing lesion sizes and enhancing behavioral recovery in animal models of ischemic and traumatic central nervous system (CNS) injury. Recent findings also suggest that neurons derived from cord stroma mesenchymal cells could alleviate movement disorders in hemiparkinsonian animal models. We review here the neurogenic potential of umbilical cord stem cells and discuss possibilities of their exploitation as an alternative to human embryonic stem cells or neural stem cells for transplantation therapy of traumatic CNS injury and neurodegenerative diseases.

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“…Cryopreservation of cells and tissue, mainly of the reproductive system, has been significantly improved lately (Woods et al, 2004), but up to now only hematopoietic stem cells have been cryopreserved (Hubel, 1997; Heng et al, 2005; Kotobuki et al, 2005) and then successfully utilized for transplantation (Broxmeyer et al, 2003; Adami et al, 2005). Moreover, to date there are no reports on the ability of either stem cells or already differentiated cells to re‐start proliferation, differentiation, and new tissue formation for therapeutic use (Korbling and Estrov, 2003).…”

mentioning

confidence: 99%

Long‐term cryopreservation of dental pulp stem cells (SBP‐DPSCs) and their differentiated osteoblasts: A cell source for tissue repair

Papaccio

Graziano

d’Aquino

et al. 2006

Journal Cellular Physiology

245

164

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It is not known whether cells derived from stem cells retain their differentiation and morpho-functional properties after long-term cryopreservation. This information is of importance to evaluate their potential for long-term storage with a view to subsequent use in therapy. Here, we describe the morpho-functional properties of dental pulp stem cells (SBP-DPSCs), and of their differentiated osteoblasts, recovered after long-term cryopreservation. After storage for 2 years, we found that stem cells are still capable of differentiation, and that their differentiated cytotypes proliferate and produce woven bone tissue. In addition, cells still express all their respective surface antigens, confirming cellular integrity. In particular, SBP-DPSCs differentiated into pre-osteoblasts, showing diffuse positivity for ALP, BAP, RUNX-2, and calcein. Recovered osteoblasts expressed bone-specific markers and were easily recognizable ultrastructurally, with no alterations observed at this level. In addition, after in vivo transplantation, woven bone converted into a 3D lamellar bone type. Therefore, dental pulp stem cells and their osteoblast-derived cells can be long-term cryopreserved and may prove to be attractive for clinical applications.

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A closed system for the clinical banking of umbilical cord blood

Cited by 8 publications

References 43 publications

Assessment of cord blood hematopoietic cell parameters before and after cryopreservation

Assessment of cord blood hematopoietic cell parameters before and after cryopreservation

Neural differentiation and potential use of stem cells from the human umbilical cord for central nervous system transplantation therapy

Long‐term cryopreservation of dental pulp stem cells (SBP‐DPSCs) and their differentiated osteoblasts: A cell source for tissue repair

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