A Clonal CD4-Positive T-Cell Line Established from the Blood of a Patient with Sézary Syndrome

Abrams, J. T.; Lessin, Stuart R.; Ghosh, Subrata; Ju, William; Vonderheid, Eric C.; Nowell̀, Peter C.; Murphy, George J.; Elfenbein, Bruce; DeFreitas, Elaine

doi:10.1111/1523-1747.ep12514693

Cited by 50 publications

(24 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…H9 is a clonal derivative of Hut78 cell line(28). MyLa, PB2B, Mac2A, SZ4, SeAx, Sez4 were a generous gift from professors K. Kaltoft and N. Ødum (Copenhagen, Denmark) and were previously described elsewhere (29–33). MJ, Hut78 cells were serially passaged in IMDM media (Invitrogen) containing 10% fetal bovine serum (FBS) (Invitrogen).…”

Section: Patients Materials and Methodsmentioning

confidence: 99%

Ectopic Expression of Cancer–Testis Antigens in Cutaneous T-cell Lymphoma Patients

Litvinov

Cordeiro

Huang

et al. 2014

Clinical Cancer Research

View full text Add to dashboard Cite

Purpose The pathogenesis of CTCL remains only partially understood. A number of recent studies attempted to identify novel diagnostic markers and future therapeutic targets. One group of antigens, cancer-testis (CT) antigens, normally present solely in testicular germ cells, can be ectopically expressed in a variety of cancers. Currently only a few studies attempted to investigate the expression of CT antigens in CTCL. Experimental Design In the present work we test the expression of CT genes in a cohort of CTCL patients, normal skin samples, skin from benign inflammatory dermatoses and in patient-derived CTCL cells. We correlate such expression with the p53 status and explore molecular mechanisms behind their ectopic expression in these cells. Results Our findings demonstrate that SYCP1, SYCP3, REC8, SPO11 and GTSF1 genes are heterogeneously expressed in CTCL patients and patient-derived cell lines, while cTAGE1 was found to be robustly expressed in both. Mutated p53 status did not appear to be a requirement for the ectopic expression of CT antigens. While T cell stimulation resulted in a significant upregulation of STAT3 and JUNB expression, it did not significantly alter the expression of CT antigens. Treatment of CTCL cells in-vitro with Vorinostat or Romidepsin Histone Deacetylase inhibitors resulted in a significant dose-dependent upregulation of mRNA, but not protein. Further expression analysis demonstrated that SYCP1, cTAGE1 and GTSF1 were expressed in CTCL, but not in normal skin or benign inflammatory dermatoses. Conclusions A number of CT genes are ectopically expressed in CTCL patients and can be used as biomarkers or novel targets for immunotherapy.

show abstract

Section: Patients Materials and Methodsmentioning

confidence: 99%

Ectopic Expression of Cancer–Testis Antigens in Cutaneous T-cell Lymphoma Patients

Litvinov

Cordeiro

Huang

et al. 2014

Clinical Cancer Research

View full text Add to dashboard Cite

show abstract

“…LCL is a lymphoblastoid B-cell line obtained from the same patient as the PB-1, 2A, and 2B lines, by in vitro infection with EpsteinBarr virus. The SZ-4 line, kindly provided by T. Abrams (Hahnemann University), was derived from a SzS patient and bears close morphological, phenotypic, and genotypic resemblance to the original tumor (43). Growth of the SZ-4 line is IL-2 dependent.…”

Section: Methodsmentioning

confidence: 99%

Activation of Jak/STAT proteins involved in signal transduction pathway mediated by receptor for interleukin 2 in malignant T lymphocytes derived from cutaneous anaplastic large T-cell lymphoma and Sezary syndrome.

Zhang

Nowak

Vonderheid

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

211

178

View full text Add to dashboard Cite

show abstract

“…54 MyLa, PB2B, Mac2A, SZ4 and SeAx were a generous gift from professor K. Kaltoft (Copenhagen, Denmark) and were previously described elsewhere. 41,[55][56][57][58] MJ, Hut78 cells were serially passaged in IMDM media (Invitrogen) containing 10% fetal bovine serum (FBS) (Invitrogen, http://www.lifetechnologies.com/ca/en/home/lifescience/cell-culture.html). HH, H9, Hut102, MyLa, Mac2A and SZ4 cells were grown in RPMI media containing 10% FBS.…”

Section: Patients and Samplesmentioning

confidence: 99%

Analysis of STAT4 expression in cutaneous T-cell lymphoma (CTCL) patients and patient-derived cell lines

et al. 2014

View full text Add to dashboard Cite

Deregulation of STAT signaling has been implicated in the pathogenesis for a variety of cancers, including CTCL. Recent reports indicate that loss of STAT4 expression is an important prognostic marker for CTCL progression and is associated with the acquisition of T helper 2 cell phenotype by malignant cells. However, little is known about the molecular mechanism behind the downregulation of STAT4 in this cancer. In the current work we test the expression of STAT4 and STAT6 via RT-PCR and/or Western Blot in CTCL lesional skin samples and in immortalized patient-derived cell lines. In these malignant cell lines we correlate the expression of STAT4 and STAT6 with the T helper (Th) phenotype markers and test the effect of Histone Deacetylase (HDAC) inhibitors and siRNA-mediated knock down of miR-155 on STAT4 expression. Our findings demonstrate that STAT4 expression correlates with Th1 phenotype, while STAT6 is associated with the Th2 phenotype. Our results further document that STAT4 and STAT6 genes are inversely regulated in CTCL. Treatment with HDAC inhibitors upregulates STAT4 expression, while at the same time decreases STAT6 expression in MyLa cells. Also, siRNA-mediated knock down of miR-155 leads to upregulation in STAT4 expression in MyLa cells. In summary, our results suggest that loss of STAT4 expression and associated switch to Th2 phenotype during Mycosis Fungoides progression may be driven via aberrant histone acetylation and/or upregulation of oncogenic miR-155 microRNA.

show abstract

A Clonal CD4-Positive T-Cell Line Established from the Blood of a Patient with Sézary Syndrome

Cited by 50 publications

References 27 publications

Ectopic Expression of Cancer–Testis Antigens in Cutaneous T-cell Lymphoma Patients

Ectopic Expression of Cancer–Testis Antigens in Cutaneous T-cell Lymphoma Patients

Activation of Jak/STAT proteins involved in signal transduction pathway mediated by receptor for interleukin 2 in malignant T lymphocytes derived from cutaneous anaplastic large T-cell lymphoma and Sezary syndrome.

Analysis of STAT4 expression in cutaneous T-cell lymphoma (CTCL) patients and patient-derived cell lines

Contact Info

Product

Resources

About