“…Although technical approaches of harvesting and grafting have been investigated to optimize the surgical protocol, methods to prepare fat prior to grafting remain controversial. Positive results have been reported with the use of fat processed by decantation, 13,14 washing with normal saline in a syringe or open air through a strainer, 15,16 centrifugation at low or high g-force, 17,18 and towel drying, in which fat is placed on a cotton towel, gauze, or Telfa to drain. 19 Supplementation with insulin, growth factors, human albumin, or plasmarich protein has also been used to improve graft retention.…”