A Clinical Study of Laotian Refugees Infected with Clonorchis Sinensis or Opisthorchis Viverrini
            
               
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Woolf, Anthony D.; Green, James; Levine, Judith A.; Estévez, E; Weatherly, Norman F.; Rosenberg, Eric S.; Frothingham, Thomas E.

doi:10.4269/ajtmh.1984.33.1279

Cited by 21 publications

(9 citation statements)

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“…Approximately nine million people are infected (World Health Organization 1995;Yossepowitch et al 2004) and approximately 67.3 million people are at risk of opisthorchiasis infection (Keiser and Utzinger 2007). The disease has increasingly presented in developed countries with the influx of Asian immigrants (Woolf et al 1984;Schwartz 1986;Peng et al 1993;Fried and Abruzzi 2010). Human strongyloidiasis is another helminthic infection caused by Strongyloides stercoralis, a worm particularly dangerous for immunosuppressed patients as well as patients undergoing corticosteroid treatment or chemotherapy (Grove 1996).…”

Section: Introductionmentioning

confidence: 98%

Rapid detection of Opisthorchis viverrini and Strongyloides stercoralis in human fecal samples using a duplex real-time PCR and melting curve analysis

et al. 2011

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Human opisthorchiasis caused by the liver fluke Opisthorchis viverrini is an endemic disease in Southeast Asian countries including the Lao People's Democratic Republic, Cambodia, Vietnam, and Thailand. Infection with the soil-transmitted roundworm Strongyloides stercoralis is an important problem worldwide. In some areas, both parasitic infections are reported as co-infections. A duplex real-time fluorescence resonance energy transfer (FRET) PCR merged with melting curve analysis was developed for the rapid detection of O. viverrini and S. stercoralis in human fecal samples. Duplex real-time FRET PCR is based on fluorescence melting curve analysis of a hybrid of amplicons generated from two genera of DNA elements: the 162 bp pOV-A6 DNA sequence specific to O. viverrini and the 244 bp 18S rRNA sequence specific to S. stercoralis, and two pairs of specific fluorophore-labeled probes. Both O. viverrini and S. stercoralis can be differentially detected in infected human fecal samples by this process through their different fluorescence channels and melting temperatures. Detection limit of the method was as little as two O. viverrini eggs and four S. stercoralis larvae in 100 mg of fecal sample. The assay could distinguish the DNA of both parasites from the DNA of negative fecal samples and fecal samples with other parasite materials, as well as from the DNA of human leukocytes and other control parasites. The technique showed 100% sensitivity and specificity. The introduced duplex real-time FRET PCR can reduce labor time and reagent costs and is not prone to carry over contamination. The method is important for simultaneous detection especially in areas where both parasites overlap incidence and is useful as the screening tool in the returning travelers and immigrants to industrialized countries where number of samples in the diagnostic units will become increasing.

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Section: Introductionmentioning

confidence: 98%

Rapid detection of Opisthorchis viverrini and Strongyloides stercoralis in human fecal samples using a duplex real-time PCR and melting curve analysis

et al. 2011

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“…2,3 In developed countries, the disease has become more prevalent with the influx of immigrants from Asia. [4][5][6] Humans are infected by eating raw or semi-cooked cyprinoid fishes harboring the O. viverrini infective stage called metacercariae. General symptoms include dyspepsia, fatigue, and upper quadrant abdominal pain.…”

Section: Introductionmentioning

confidence: 99%

Rapid Molecular Detection of Opisthorchis viverrini in Human Fecal Samples by Real-Time Polymerase Chain Reaction

Intapan

Thanchomnang²,

Lulitanond³

et al. 2009

The American Journal of Tropical Medicine and Hygiene

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Abstract. Real-time fluorescence resonance energy transfer (FRET) polymerase chain reaction (PCR) supplemented with melting curve analysis is a highly sensitive and fast method offering a high throughput. We report the development of a real-time FRET PCR for molecular detection of Opisthorchis viverrini in human fecal samples. The diagnostic sensitivity, specificity, accuracy, and positive and negative predictive values of this method were 97.5%, 100%, 98.9%, 100%, and 98.2%, respectively. The sensitivity was not significantly different from that of the quantified formalin-ethyl acetate concentration technique, the gold standard ( P > 0.05). The procedure has potential for diagnosis of human opisthorchiasis in disease-endemic areas, for large epidemiologic investigations involving at risk populations, and monitoring eradication programs of the liver fluke, which causes hepatobiliary diseases and induces cholangiocarcinoma.

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“…Opisthorchiasis is a liver fluke infection, found mainly in Southeast Asia (8) but increasingly in developed countries due to an influx of Asian immigrants (7). The diagnostic methods are based on the demonstration of eggs in stools, although there are still difficulties in distinguishing eggs from heterophyid and lecithodendriid parasites (2).…”

mentioning

confidence: 99%

Detection of Opisthorchis viverrini in Human Stool Specimens by PCR

et al. 2002

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A Clinical Study of Laotian Refugees Infected with Clonorchis Sinensis or Opisthorchis Viverrini *

Cited by 21 publications

References 0 publications

Rapid detection of Opisthorchis viverrini and Strongyloides stercoralis in human fecal samples using a duplex real-time PCR and melting curve analysis

Rapid detection of Opisthorchis viverrini and Strongyloides stercoralis in human fecal samples using a duplex real-time PCR and melting curve analysis

Rapid Molecular Detection of Opisthorchis viverrini in Human Fecal Samples by Real-Time Polymerase Chain Reaction

Detection of Opisthorchis viverrini in Human Stool Specimens by PCR

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