Detection of
            <i>Opisthorchis viverrini</i>
            in Human Stool Specimens by PCR

Wongratanacheewin, Surasakdi; Pumidonming, Wilawan; Sermswan, Rasana W.; Pipitgool, Vichit; Maleewong, Wanchai

doi:10.1128/jcm.40.10.3879-3880.2002

Cited by 83 publications

(44 citation statements)

References 5 publications

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“…Alternatively, species-specific PCR-based techniques have been developed. [39][40][41] However, PCR methods are less suitable for large-scale community-based investigations as they are costly and still of limited direct applicability under field conditions.…”

Section: Discussionmentioning

confidence: 99%

Diversity of human intestinal helminthiasis in Lao PDR

Sayasone

Vonghajack

Vanmany

et al. 2009

Transactions of the Royal Society of Tropical Medicine and Hygi

108

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Summary Food-borne trematodiasis is an emerging public health problem, including in Lao PDR. We investigated the diversity of intestinal helminthes and polyparasitism in patients with hepatobiliary or intestinal symptoms in hospital and community-based surveys. Stool samples from 232 individuals aged ≥15 years were examined by the Kato-Katz method (three samples) and a formalin ethyl-acetate concentration technique (one sample). Opisthorchis viverrini and minute intestinal flukes (MIF) were common, with prevalences of 86.2% and 62.9%, respectively. Hookworm was the predominant soil-transmitted helminth (65.9%). The prevalences of Taenia spp., Strongyloides stercoralis and Trichuris trichiura were 22.8%, 10.3% and 8.6%, respectively. Additionally, 97 individuals were purged; O. viverrini and Haplorchis taichui were found in 95 and 76 participants, respectively. Other trematodes included Phaneropsolus bonnei (22.7%), Prosthodendrium molenkampi (14.4%), Haplorchis pumilio (5.2%), Haplorchis yokogawai (3.1%) and Echinochasmus japonicus (3.1%). Co-infection with O. viverrini and MIFs was rampant (81.4%). Polytrematode infection is highly prevalent in Lao PDR and hence requires urgent attention.

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Section: Discussionmentioning

confidence: 99%

Diversity of human intestinal helminthiasis in Lao PDR

Sayasone

Vonghajack

Vanmany

et al. 2009

Transactions of the Royal Society of Tropical Medicine and Hygi

108

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“…Detection of O. viverrini -specific sequences by cPCR for the diagnosis of human opisthorchiasis in fecal samples has yielded a varying sensitivity, [13][14][15][16] which shows the need for a more rapid, sensitive, and specific method. We used OV-F and OV-R primers for diagnosis of O. viverrini infection in human fecal specimens by cPCR.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, this method is time-consuming. To overcome the constraints of the microscopic method, various sensitive and specific molecular methods such as classic polymerase chain reaction (cPCR)-based techniques capable of DNA amplification from helminthic eggs in stool samples have been reported, [12][13][14][15][16] but these methods still lack the desired reliability.…”

Section: Introductionmentioning

confidence: 99%

Rapid Molecular Detection of Opisthorchis viverrini in Human Fecal Samples by Real-Time Polymerase Chain Reaction

Intapan

Thanchomnang²,

Lulitanond³

et al. 2009

The American Journal of Tropical Medicine and Hygiene

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Abstract. Real-time fluorescence resonance energy transfer (FRET) polymerase chain reaction (PCR) supplemented with melting curve analysis is a highly sensitive and fast method offering a high throughput. We report the development of a real-time FRET PCR for molecular detection of Opisthorchis viverrini in human fecal samples. The diagnostic sensitivity, specificity, accuracy, and positive and negative predictive values of this method were 97.5%, 100%, 98.9%, 100%, and 98.2%, respectively. The sensitivity was not significantly different from that of the quantified formalin-ethyl acetate concentration technique, the gold standard ( P > 0.05). The procedure has potential for diagnosis of human opisthorchiasis in disease-endemic areas, for large epidemiologic investigations involving at risk populations, and monitoring eradication programs of the liver fluke, which causes hepatobiliary diseases and induces cholangiocarcinoma.

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“…Stool antigen tests for Clonorchis and Opisthorchis have also been developed, and may be of use in detection of light infections (85) . Stool PCR assays of O. viverrini DNA have also been developed with 100% sensitivity and 98% specificity in heavy infections, and although less sensitive for light infections (68%) (86) , may enable detection of light or early infection when stool microscopy is negative (85). Access to serological, antigen and PCR diagnostics for Clonorchis and Opisthorchis varies, and validation of these tests for widespread use is still required.…”

Section: Immunodiagnosis and Molecular Diagnosticsmentioning

confidence: 99%