A Clinical and Endocrine Study on the Effect of Orally Administered Bacterial Endotoxin in Adult Pigs and Goats

Cort, N.; Fredriksson, G.; Kindahl, H.; Edqvist, L.‐E.; Rylander, Ragnar

doi:10.1111/j.1439-0442.1990.tb00884.x

Cited by 44 publications

(44 citation statements)

References 22 publications

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“…The level of endotoxins in the product (2.14-5.81 IU/mg) is similar to or lower than that observed in other feedingstuffs and is therefore of no concern for the target species (Cort et al, 1990). Nevertheless, in the case of nutritional additives produced by fermentation, the risks associated with the residues from the fermentation process in the final product need to be assessed.…”

Section: Safety For the Target Speciesmentioning

confidence: 78%

Safety and efficacy of l‐threonine produced by fermentation using Escherichia coli CGMCC 7.232 for all animal species

Additives¹,

Bampidis²,

Azimonti³

et al. 2018

EFS2

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The product subject of this assessment is l‐threonine produced by fermentation with a genetically modified strain of Escherichia coli (CGMCC 7.232). It is intended to be used in feed and water for drinking for all animal species and categories. The production strain and its recombinant DNA were not detected in the additive. The product l‐threonine, manufactured by fermentation with E. coli CGMCC 7.232, does not raise any safety concern with regard to the genetic modification of the production strain. l‐Threonine produced using E. coli CGMCC 7.232 is considered safe for the target species. The FEEDAP Panel has concerns regarding the safety of the simultaneous administration of l‐threonine via water for drinking and feed. l‐Threonine produced using E. coli CGMCC 7.232 is safe for the consumer. In absence of data, the FEEDAP Panel cannot conclude on the potential of the additive to be irritant to skin and eyes or to be a skin sensitiser. There is a risk from the inhalation exposure to endotoxins for persons handling the additive. l‐Threonine produced using E. coli CGMCC 7.232 is safe for the environment. The product under assessment is considered an efficacious source of the amino acid l‐threonine for all animal species. For l‐threonine to be as efficacious in ruminants as in non‐ruminant species, it requires protection against degradation in the rumen.

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Section: Safety For the Target Speciesmentioning

confidence: 78%

Safety and efficacy of l‐threonine produced by fermentation using Escherichia coli CGMCC 7.232 for all animal species

Additives¹,

Bampidis²,

Azimonti³

et al. 2018

EFS2

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“…This is the case for the L-threonine produced by E. coli KCCM11133P, which contains ≥99.4 % threonine and less than 1 % unidentified material on a dry matter basis. The level of endotoxins in the product (18 to 30 IU/mg) is negligible in comparison to that observed in other feedingstuffs (1 000 IU/mg; Cort et al, 1990) and is therefore of no concern for the target species. No safety concerns arose from the genetic modification.…”

Section: Safety For the Target Speciesmentioning

confidence: 85%

Scientific Opinion on the safety and efficacy of l‐threonine produced by Escherichia coli strains NRRL B‐30843, DSM 26131, KCCM11133P or DSM 25085 for all animal species based on a dossier submitted by AMAC EEIG

Feed¹

2015

EFS2

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This opinion concerns L-threonine as a feed additive produced by four different strains derived from Escherichia coli K-12. Three strains are genetically modified (GM): NRRL B-30843, KCCM11133P and DSM 26131. L-Threonine produced by E. coli DSM 26131 could not be assessed because of the insufficient molecular characterisation of the genetic modification, and the lack of data on both the absence of the production strain and its recombinant DNA from the final product. No safety concerns were found in the products related to the genetic modification of the other GM strains or to antibiotic resistance of the producer strains. L-Threonine products made by fermentation using E. coli strains NRRL B-30843, KCCM11133P and DSM 25085 are free of the production strain and have a high purity (≥ 98.8 %). L-Threonine, technically pure, produced by E. coli strains NRRL B-30843, KCCM11133P and DSM 25085 is safe for the target animals when used in appropriate amounts to supplement threonine-deficient feeds, for the consumer of animal products and for the environment. The FEEDAP Panel considers that L-threonine produced by E. coli strains NRRL B-30843, KCCM11133P or DSM 25085 is not an irritant to eyes and skin, and is not a skin sensitiser. There is no risk from inhalation of L-threonine, but concerns may arise from the content of endotoxins in the products. These L-threonine products are considered an efficacious source of the amino acid L-threonine for all animal species. For L-threonine to be as efficacious in ruminants as in non-ruminant species, it requires protection against degradation in the rumen. The Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) has concerns regarding the safety of the simultaneous oral administration of L-threonine via water for drinking and feed. L-Threonine is an essential amino acid for all animal species. It is commonly considered the second and third limiting amino acid in cereal-based diets for pigs and poultry, respectively. It is widely used in the feed industry to optimise dietary protein.Three of the strains are GM: E. coli NRRL B-30843, E. coli DSM 26131 and E. coli KCCM11133P. The final product obtained from E. coli strain NRRL B-30843 contains no cultivable production organisms or recombinant DNA. The product from E. coli strain KCCM11133P contains no cultivable production organisms, but the possible presence of fragments spanning the full length of recombinant genes cannot be ruled out. However, no genes of concern remain in the production strain KCCM11133P. No safety concerns were found in these two products related to the genetic modification. The genetic modification of E. coli DSM 26131 and its possible consequences for the product could not be assessed because of insufficient data.L-Threonine products made by fermentation with E. coli strains NRRL B-30843, KCCM11133P or DSM 25085 are free of the production strain and have a high purity (≥ 98.8 %). No recombinant DNA could be detected in L-threonine produced by fermentation of the GM strains E. col...

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“…These values are compared with ca 1,000 IU/mg commonly found in feedingstuffs (Cort et al, 1990). Such tolerance studies with a certain indispensable amino acid will inevitably result in amino acid imbalances, with depression of feed intake and hence impaired performance and increased nitrogen excretion.…”

Section: Safety For the Target Speciesmentioning

confidence: 91%

Safety and efficacy of l‐tryptophan produced by fermentation with Escherichia coli CGMCC 7.248 for all animal species

Bampidis¹,

Azimonti²,

Bastos³

et al. 2019

EFS2

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Following a request from the European Commission, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on L-tryptophan produced by fermentation with a genetically modified strain of Escherichia coli CGMCC 7.248 when used as a nutritional additive in feed and water for drinking for all animal species and categories. The production strain E. coli CGMCC 7.248 and its recombinant DNA were not detected in the final product. The product L-tryptophan, manufactured by fermentation with E. coli CGMCC 7.248, does not give rise to any safety concern with regard to the genetic modification of the production strain. L-Tryptophan produced by E. coli CGMCC 7.248 is safe for non-ruminant target species. The use of unprotected L-tryptophan in ruminant feed should be avoided. L-Tryptophan produced by fermentation by E. coli CGMCC 7.248 is safe for the consumer. The level of endotoxins present in the product and its dusting potential indicate an inhalation risk for the user. L-Tryptophan produced by E. coli CGMCC 7.248 is not a skin or eye irritant but it is a dermal sensitiser. The use of L-tryptophan produced by E. coli CGMCC 7.248 in animal nutrition does not pose a risk to the environment. The product under assessment is regarded as an effective source of the amino acid L-tryptophan for all non-ruminant species. For the supplemental L-tryptophan to be as efficacious in ruminants as in non-ruminant species, it requires protection against degradation in the rumen.

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A Clinical and Endocrine Study on the Effect of Orally Administered Bacterial Endotoxin in Adult Pigs and Goats

Cited by 44 publications

References 22 publications

Safety and efficacy of l‐threonine produced by fermentation using Escherichia coli CGMCC 7.232 for all animal species

Safety and efficacy of l‐threonine produced by fermentation using Escherichia coli CGMCC 7.232 for all animal species

Scientific Opinion on the safety and efficacy of l‐threonine produced by Escherichia coli strains NRRL B‐30843, DSM 26131, KCCM11133P or DSM 25085 for all animal species based on a dossier submitted by AMAC EEIG

Safety and efficacy of l‐tryptophan produced by fermentation with Escherichia coli CGMCC 7.248 for all animal species

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