A Class IV Adenylate Cyclase, CyaB, Is Required for Capsule Polysaccharide Production and Biofilm Formation in Vibrio parahaemolyticus

Regmi, Abish; Tague, J G; Lichty, Katherine E. Boas; Boyd, E. Fidelma

doi:10.1128/aem.01874-22

Cited by 2 publications

(1 citation statement)

References 72 publications

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“…In support of this positive role in CPS and biofilm, in the Δ opaR, both the laf and scrABC operons were highly expressed and cells produce hyper swarming cells showing OpaR is a negative regulator of swarming motility (10, 30, 61, 63). A recent study showed that a crp deletion mutant, which encodes CRP, had a defect in CPS and biofilm formation indicating a positive role in this phenotype (65).…”

Section: Discussionmentioning

confidence: 99%

Regulators H-NS and LeuO inversely control swarming motility and biofilm formation inVibrio parahaemolyticus

Bhide,

Tague,

Andrews

et al. 2024

Preprint

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Vibrio parahaemolyticusis a halophile present in marine environments worldwide and is a leading cause of bacterial seafood-borne gastroenteritis. Free livingVibrio parahaemolyticusplanktonic cells can either attach to surfaces to form swarming cells or develop into a sessile three-dimensional biofilm structure. Swarming motility requires lateral flagella (lafoperon) and the expression of the surface sensing operonscrABCto produce a spreading cauliflower colony morphology. Biofilms are formed from capsule polysaccharide (CPS) encoded by thecpsA-Koperon that is positively regulated by CpsR and CpsQ. In enteric bacteria, H-NS is a global gene silencer and LeuO is an antagonist of H-NS. In this work, we examined the role of these regulators in the decision between swarming and biofilm behaviors using deletion mutants ofleuO, hns,and a double deletionleuO/hns. The wild type and ΔleuOstrains produced swarming colonies whereas Δhnsproduced a hyper swarming whereas in ΔleuO/Δhns,the phenotype reverted to wild type. Transcriptional reporter assays of PlafB-gfpand PscrA-gfpshowed significantly increased fluorescence in Δhnscompared to wild type. In the ΔleuO/Δhnsmutant, PlafB-gfpfluorescence reverted to wild type levels and PscrA-gfpshowed increased fluorescence compared to wild type. In CPS assays, ΔleuOhad a less dense rugose morphology compared to wild type and Δhnsproduced a smooth colony, which also produced significantly less biofilm. ΔleuO/Δhnshad an opaque morphology and produced significantly more biofilm. Reporter expression assays of PcpsA-gfpand PcpsR-gfpconfirmed the roles of both H-NS and LeuO in CPS and biofilm formation.ImportanceThis study determined the role of LeuO and H-NS in controlling the decision between two surface based behaviors, swarming motility and sessile biofilm formation inV. parahaemolyticus. The effects of deletions ofleuO,hns,and a double ΔleuO/Δhnsmutant showed that H-NS was a negative regulator of swarming, but a positive regulator of biofilm formation. The mechanism of this control was in part due to H-NS inhibition of LeuO at loci required for swarming and biofilm formation.

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Section: Discussionmentioning

confidence: 99%

Regulators H-NS and LeuO inversely control swarming motility and biofilm formation inVibrio parahaemolyticus

Bhide,

Tague,

Andrews

et al. 2024

Preprint

Self Cite

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Transcriptomics analysis of the mechanism behind Lactobacillus rhamnosus Gr18 biofilm formation in an Mn2+-deficient environment

Liu,

Shen,

Liu

et al. 2024

LWT

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A Class IV Adenylate Cyclase, CyaB, Is Required for Capsule Polysaccharide Production and Biofilm Formation in Vibrio parahaemolyticus

Abstract: Here, we characterized the roles of CRP and CyaA in V. parahaemolyticus , showing that cAMP-CRP is an activator of metabolism, motility, capsule production, and biofilm formation. These results are in contrast to cAMP-CRP in V. cholerae , which represses capsule and biofilm formation.

Cited by 2 publications

References 72 publications

Regulators H-NS and LeuO inversely control swarming motility and biofilm formation inVibrio parahaemolyticus

Regulators H-NS and LeuO inversely control swarming motility and biofilm formation inVibrio parahaemolyticus

Transcriptomics analysis of the mechanism behind Lactobacillus rhamnosus Gr18 biofilm formation in an Mn2+-deficient environment

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