A citrus abscission agent induces anoxia- and senescence-related gene expression in Arabidopsis

Alférez, Fernando; Zhong, Guang Yan; Burns, J. C.

doi:10.1093/jxb/erm111

Cited by 17 publications

(13 citation statements)

References 99 publications

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“…It is long known that exogenous application of ethylene at a wide range of concentrations promotes leaf and fruit abscission in citrus, and that ethylene biosynthesis and action inhibitors can effectively suppress or delay abscission (Huberman et al 1983;Brown 1997;Zhong et al 2001a;Alferez et al 2007). In contrast, exogenous application of auxins and GAs can inhibit abscission in citrus (Goren 1993) and has thus long been used to prevent young fruit from abnormal abscission (Agustí et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Profiling gene expression in citrus fruit calyx abscission zone (AZ-C) treated with ethylene

et al. 2015

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On-tree storage and harvesting of mature fruit account for a large proportion of cost in the production of citrus, and a reduction of the cost would not be achieved without a thorough understanding of the mechani sm of the mature fruit abscission. Genome-wide gene expression changes in ethylene-treated fruit calyx abscission zone (AZ-C) of Citrus sinensis cv. Olinda were therefore investigated using a citrus genome array representing up to 33,879 citrus transcripts. In total, 1313 and 1044 differentially regulated genes were identified in AZ-C treated with ethylene for 4 and 24 h, respectively. The results showed that mature citrus fruit abscission commenced with the activation of ethylene signal transduction pathway that led to the activation of ethylene responsive transcription factors and the subsequent transcriptional regulation of a large set of ethylene responsive genes. Significantly down-regulated genes included those of starch/sugar biosynthesis, transportation of water and growth promoting hormone synthesis and signaling, whereas significantly up-regulated genes were those involved in defense, cell wall degradation, and secondary metabolism. Our data unraveled the underlying mechanisms of some known important biochemical events occurring at AZ-C and should provide informative suggestions for future manipulation of the events to achieve a controllable abscission for mature citrus fruit.

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Section: Introductionmentioning

confidence: 99%

Profiling gene expression in citrus fruit calyx abscission zone (AZ-C) treated with ethylene

et al. 2015

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“…1996; Holk et al . 2002) and other lipid‐signalling pathway‐related genes were induced in the vegetative portion of Arabidopsis plants showing symptoms of plant decline in response to root applications of CMNP (Alferez & Burns 2003). Factors such as hyperosmotic stress (Meijer et al .…”

Section: Introductionmentioning

confidence: 99%

Citrus abscission and Arabidopsis plant decline in response to 5‐chloro‐3‐methyl‐4‐nitro‐1H‐pyrazole are mediated by lipid signalling

Alférez

Singh

Umbach

et al. 2005

Plant Cell & Environment

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The compound 5-chloro-3-methyl-4-nitro-1 H -pyrazole (CMNP) is a pyrazole-derivative that induces abscission selectively in mature citrus ( Citrus sinensis ) fruit when applied to the canopy and has herbicidal activity on plants when applied to roots. Despite the favourable efficacy of this compound, the mode of action remains unknown. To gain information about the mode of action of CMNP, the effect of application to mature citrus fruit and Arabidopsis thaliana roots was explored. Peel contact was essential for mature fruit abscission in citrus, whereas root drenching was essential for symptom development and plant decline in

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“…The highly significant differential expression level of trehalose-6-phosphatase suggests a role for this gene in abscission induction. A role for this protein as a signal induced by nutritional stress had previously been suggested by Alferez et al (2007) and Botton et al (2011).…”

Section: Az Development Is Controlled By a Complex Gene Networkmentioning

confidence: 81%

Additional Amphivasal Bundles in Pedicel Pith Exacerbate Central Fruit Dominance and Induce Self-Thinning of Lateral Fruitlets in Apple

et al. 2014

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Apple (Malus 3 domestica) trees naturally produce an excess of fruitlets that negatively affect the commercial value of fruits brought to maturity and impact their capacity to develop flower buds the following season. Therefore, chemical thinning has become an important cultural practice, allowing the selective removal of unwanted fruitlets. As the public pressure to limit the use of chemical agents increases, the control of thinning becomes a major issue. Here, we characterized the self-thinning capacity of an apple hybrid genotype from the tree scale to the molecular level. Additional amphivasal vascular bundles were identified in the pith of pedicels supporting the fruitlets with the lowest abscission potential (central fruitlet), indicating that these bundles might have a role in the acquisition of dominance over lateral fruitlets. Sugar content analysis revealed that central fruitlets were better supplied in sorbitol than lateral fruitlets. Transcriptomic profiles allowed us to identify genes potentially involved in the overproduction of vascular tissues in central pedicels. In addition, histological and transcriptomic data permitted a detailed characterization of abscission zone development and the identification of key genes involved in this process. Our data confirm the major role of ethylene, auxin, and cell wall-remodeling enzymes in abscission zone formation. The shedding process in this hybrid appears to be triggered by a naturally exacerbated dominance of central fruitlets over lateral ones, brought about by an increased supply of sugars, possibly through additional amphivasal vascular bundles. The characterization of this genotype opens new perspectives for the selection of elite apple cultivars.

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A citrus abscission agent induces anoxia- and senescence-related gene expression in Arabidopsis

Cited by 17 publications

References 99 publications

Profiling gene expression in citrus fruit calyx abscission zone (AZ-C) treated with ethylene

Profiling gene expression in citrus fruit calyx abscission zone (AZ-C) treated with ethylene

Citrus abscission and Arabidopsis plant decline in response to 5‐chloro‐3‐methyl‐4‐nitro‐1H‐pyrazole are mediated by lipid signalling

Additional Amphivasal Bundles in Pedicel Pith Exacerbate Central Fruit Dominance and Induce Self-Thinning of Lateral Fruitlets in Apple

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