A Chiral‐Nanoassemblies‐Enabled Strategy for Simultaneously Profiling Surface Glycoprotein and MicroRNA in Living Cells

Abstract

Assemblies of nanomaterials for biological applications in living cells have attracted much attention. Herein, graphene oxide (GO)-gold nanoparticle (Au NP) assemblies are driven by a splint DNA strand, which is designed with two regions at both ends that are complementary with the DNA sequence anchored on the surface of the GO and the Au NPs. In the presence of microRNA (miR)-21 and epithelial cell-adhesion molecule (EpCAM), the hybridization of miR-21 with a molecular probe leads to the separation of 6-fluorescein-phosphoramidite-modified Au NPs from GO, resulting in a decrease in the Raman signal, while EpCAM recognition reduces circular dichroism (CD) signals. The CD signals reverse from negative in original assemblies into positive when reacted with cells, which correlates with two enantiomer geometries. The EpCAM detection has a good linear range of 8.47-74.78 pg mL and a limit of detection (LOD) of 3.63 pg mL , whereas miR-21 detection displays an outstanding linear range of 0.07-13.68 amol ng and LOD of 0.03 amol ng . All the results are in good agreement with those of the Raman and confocal bioimaging. The strategy opens up an avenue to allow the highly accurate and reliable diagnosis (dual targets) of clinic diseases.

The Fabrication of rGO/(PLL/PASP)3@DOX Nanorods with pH‐Switch for Photothermal Therapy and Chemotherapy

“…[2][3] Recently,n anoscale drug carriers have been reported as an effective methodt oc ircumvent MDR and they offer ar emarkable intracellulard rug delivery mechanism to improvea nticancer efficacy. [4][5][6][7][8][9][10] Reduced graphene-based drug-delivery systems have attracted the most attention because of their unique physical, electrical and chemicalp roperties, andt hey have been widely used as biomedical treatments, particularly for use in photothermal therapy( PTT) and cell imaging. [11][12][13][14][15][16][17][18][19][20][21][22] PTT is one of the mostp rominentm ethods for the application of rGO-based nanodrug systems.…”

Chiral Shell Core–Satellite Nanostructures for Ultrasensitive Detection of Mycotoxin

“…Conversely, a CD signal can produce a significant change in signal intensity with no change in absorption. This is due to the fact that long‐range coupling can produce chiral signals, which is consistent with our previous findings . The surface electrical field distribution maps for shell NP, Au NP, and core–satellite assembly are shown in Figure C–E.…”

“…This is due to the fact that long-range coupling can produce chiral signals, which is consistent with our previous findings. [43,44] The surface electrical field distribution maps for shell NP, Au NP, and core-satellite assembly are shown in Figure 2C-E. With the integration of a gap in single-shell NPs (E-field 11 V m −1 ), the surrounded Au NPs on shell NPs (shell-satellite assemblies) gave rise to the strongest E-field of 29 V m −1 . The electric field for NPs (15 nm) assembled around Au NPs (25 nm) was 17 V m −1 , which was due to an enhancement of the electric field by Au NP-Au NP gap junctions.…”

Chiral thiophene derivatives with optimal two‐photon absorption in near‐infrared window I and II

“…Under those modulations, the molecules will show nonlinear chiroptical behaviors, including the difference in TPA or two‐photon emission under the excitation of left and right circularly polarized light. It has been revealed in previous literature that linear electron circular dichroism (ECD) is an important fingerprinting tool and commonly applied in biological research . Compared to ECD, two‐photon circular dichroism (TPCD) is the difference in TPA, which can provide complementary structural and conformational information .…”

“…It has been revealed in previous literature that linear electron circular dichroism (ECD) is an important fingerprinting tool and commonly applied in biological research. [15] Compared to ECD, two-photon circular dichroism (TPCD) is the difference in TPA, which can provide complementary structural and conformational information. [16][17][18] Although they are promising in biochemistry, pharmacy industry and photonic, chiral molecules with strong TPCD have been under scarce investigation.…”