A chimeric cry8Ea1 gene flanked by MARs efficiently controls Holotrichia parallela

Li, Geng; Chi, Jing; Shu, Changlong; Gresshoff, Peter M.; Song, Fuping; Huang, Dafang; Zhang, Jie

doi:10.1007/s00299-013-1417-2

Cited by 13 publications

(7 citation statements)

References 28 publications

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“…Transgenes flanked with MARs were found responsible in forming their own chromatic domain and thus become insulated from the influence of factors in the chromatin adjacent to site of insertion that ascribed to reduce the effect of gene silencing and causing enhanced levels of transgene expression (Hall et al, 1991). Expression cry8Ea1 gene flanked with tobacco-derived two MARs sequences increased the transcription of mRNA and expression of transgenic proteins to an extent of 9.0 and 2.5 times, respectively, in peanut (Geng et al, 2013). These studies imply that MARs sequences are more potential for promoting the transcription rates at level of mRNA than protein synthesis that might be due to their involvement in T-DNA region to form more accessible chromatin structure leading to RNA polymerase and associated transcription factors to bind DNA for transcription initiation.…”

Section: Insect Pestsmentioning

confidence: 99%

Progress in genetic engineering of peanut (Arachis hypogaea L.)—A review

Krishna

Singh

Kim

et al. 2015

Plant Biotechnology Journal

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SummaryPeanut (Arachis hypogaea L.) is a major species of the family, Leguminosae, and economically important not only for vegetable oil but as a source of proteins, minerals and vitamins. It is widely grown in the semi-arid tropics and plays a role in the world agricultural economy. Peanut production and productivity is constrained by several biotic (insect pests and diseases) and abiotic (drought, salinity, water logging and temperature aberrations) stresses, as a result of which crop experiences serious economic losses. Genetic engineering techniques such as Agrobacterium tumefaciens and DNA-bombardment-mediated transformation are used as powerful tools to complement conventional breeding and expedite peanut improvement by the introduction of agronomically useful traits in high-yield background. Resistance to several fungal, virus and insect pest have been achieved through variety of approaches ranging from gene coding for cell wall component, pathogenesis-related proteins, oxalate oxidase, bacterial chloroperoxidase, coat proteins, RNA interference, crystal proteins etc. To develop transgenic plants withstanding major abiotic stresses, genes coding transcription factors for drought and salinity, cytokinin biosynthesis, nucleic acid processing, ion antiporter and human antiapoptotic have been used. Moreover, peanut has also been used in vaccine production for the control of several animal diseases. In addition to above, this study also presents a comprehensive account on the influence of some important factors on peanut genetic engineering. Future research thrusts not only suggest the use of different approaches for higher expression of transgene(s) but also provide a way forward for the improvement of crops.

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Section: Insect Pestsmentioning

confidence: 99%

Progress in genetic engineering of peanut (Arachis hypogaea L.)—A review

Krishna

Singh

Kim

et al. 2015

Plant Biotechnology Journal

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“…Subsequently, Jia et al () constructed BIOT1858G by introducing the cry8Ga1 ‐containing plasmid pSTK‐8G into the BIOT185 engineered strain, which made the engineered strain toxic to H. oblita . Furthermore, Geng et al () expressed the Cry8Ea protein in a genetically modified peanut to evaluate its efficiency in controlling Scarabaeidae pests. Both the Bt insecticides and transgenic peanuts were shown to be effective against Scarabaeidae larvae in lab experiments, however, under natural living conditions, the insecticidal‐protein‐damaged Scarabaeidae larvae were able to survive by refusing to feed on Bt insecticides or transgenic peanuts and instead fed on other organic matter in the soil.…”

Section: Introductionmentioning

confidence: 99%

Effective bacterial insecticidal proteins against coleopteran pests: A review

Wang

Shu

Zhang

2019

Arch Insect Biochem Physiol

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Coleoptera, the order of insects commonly referred to as beetles, are able to survive in various environments, and thus, comprise the largest order in the animal kingdom. Coleopterans mainly include coprophagous and phytophagous lineages, and many species of the latter lineage are serious pests. In addition to traditional chemical methods, biocontrol measures using various bacterial insecticidal proteins have also gradually been developed to control these insect pests. In this review, we summarized the possible coleopteran‐pest‐specific bacteria and insecticidal proteins that have been reported in the literature thus far and have provided a comprehensive overview and long‐term guidance for the control of coleopteran pests in the future.

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“…A novel cry8 gene exhibiting activity against the larvae of Holotrichia oblita and H. parallela was identified [10]. Furthermore, a chimeric cry8Ea1 gene flanked by MARs in transgenic peanut plants effectively controls H. parallela [11], but no studies have investigated transgenic soybean plants expressing cry8 -like genes.…”

Section: Introductionmentioning

confidence: 99%

Soybean plants expressing the Bacillus thuringiensis cry8-like gene show resistance to Holotrichia parallela

et al. 2019

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Background Cry8-like from Bacillus thuringiensis (Bt) encodes an insecticidal crystal (Cry) protein. Holotrichia parallela (Coleoptera: Scarabaeoidae), commonly known as the dark black chafer, is a troublesome pest of soybean (Glycine max). To test whether cry8-like can confer resistance against H. parallela to soybean, we introduced cry8-like from the Bt strain HBF-18 into soybean cultivar Jinong 28. Results Quantitative reverse transcription-PCR analysis demonstrated that cry8-like was expressed most highly in soybean leaves. In addition, Southern blot assays revealed that one copy of the integrated fragment was present in the transformed plants. Eight independent cry8-like transgenic lines were subsequently fed on by H. parallela. Under H. parallela feeding stress, the survival rates of the non-transgenic plants were 92% lower than those of the transgenic plants. The mortality rate of H. parallela increased when the larvae fed on the roots of T1 transgenic soybean plants. Moreover, the surviving larvae were deformed, and their growth was inhibited. Conclusions Collectively, our data suggest that transgenic soybean plants expressing the cry8-like gene are more resistant to H. parallela than non-transgenic plants and that transgenic expression of the cry8-like gene may represent a promising strategy for engineering pest tolerance. The events generated in this study could thus be utilized in soybean breeding programs.

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A chimeric cry8Ea1 gene flanked by MARs efficiently controls Holotrichia parallela

Cited by 13 publications

References 28 publications

Progress in genetic engineering of peanut (Arachis hypogaea L.)—A review

Progress in genetic engineering of peanut (Arachis hypogaea L.)—A review

Effective bacterial insecticidal proteins against coleopteran pests: A review

Soybean plants expressing the Bacillus thuringiensis cry8-like gene show resistance to Holotrichia parallela

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