A chicken leptin-specific radioimmunoassay

Dridi, Sami; Williams, John; Bruggeman, Veerle; Onagbesan, M; Raver, N; Decuypere, Eddy; Djiane, Jean; Gertler, Arieh; Taouis, Mohammed

doi:10.1016/s0739-7240(00)00053-9

Cited by 32 publications

(14 citation statements)

References 23 publications

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“…In individuals with different fat scores, the mean levels of the protein detected by anti-leptin antibodies (leptin) were 2.42 ng/ml (range from 1.19 to 3.83 ng/ml). These values correspond with data derived both from the chicken (Dridi et al, 2000a) and from wild birds (Quillfeldt et al, 2009). In thinbilled prion Pachyptila belcheri nestlings, leptin levels in blood fluctuated between 1 and 3 ng/ml (Quillfeldt et al, 2009), a range comparable with values presented here for dunlins.…”

Section: Discussionsupporting

confidence: 89%

“…On the other hand, there are several reports demonstrating the presence of leptin (Taouis et al, 1998;Ashwell et al, 1999a;Kochan et al, 2006a;Quillfeldt et al, 2009) and its receptor (Richards and Poch, 2003;Liu et al, 2007;Ohkubo et al, 2007;Cerasale et al, 2011) in wild and domestic birds. Assays based on antibody-antigen interactions enable detection of leptin or at least leptin-like protein, which is very similar in size and structure (Dridi et al, 2000a;Kochan et al, 2006a;Neglia et al, 2007;Quillfeldt et al, 2009;Kordonowy et al, 2010). Leptin-induced activation of the JAK-STAT pathway (the main signaling pathway initiated by leptin in mammals) has also been demonstrated (Adachi et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Circulating leptin levels do not reflect the amount of body fat in the dunlin Calidris alpina during migration

Gogga

Karbowska

Kochan

et al. 2013

General and Comparative Endocrinology

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Circulating leptin levels do not reflect the amount of body fat in the dunlin Calidris alpina during migration

Gogga

Karbowska

Kochan

et al. 2013

General and Comparative Endocrinology

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“…Plasma leptin (Dridi et al, 2000a) and insulin (Simon et al,'74) levels were determined by RIA and intra-assay coefficients of variation were 4.7 and 3.9% respectively.…”

Section: Plasma Glucose Leptin and Insulin Measurementsmentioning

confidence: 99%

Acute cold‐ and chronic heat‐exposure upregulate hepatic leptin and muscle uncoupling protein (UCP) gene expression in broiler chickens

Dridi

Temim²,

Derouet³

et al. 2008

J. Exp. Zool.

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Emerging evidence showed that variations in environmental temperature affect both leptin and uncoupling protein (UCP) gene expression in mammals, whereas a little is known about such interactions in birds. Thus, we conducted the present study to investigate the influence of acute (2 hours) cold (4 degrees C) and chronic (10 days) heat (32 degrees C) exposure on hepatic leptin and muscle UCP gene expression in 5-wk-old broiler chickens. Both cold- and heat-exposure significantly (P < 0.05 to P < 0.001) upregulated hepatic leptin (by 35 and 46%, respectively) and muscle UCP mRNA levels (by 71 and 71%, respectively) compared to the thermoneutrality (22 degrees C). This result suggests that leptin and UCP may be involved in the thermoregulation response of chickens to extreme climate (cold and hot temperatures). The upregulation of hepatic leptin gene expression was accompanied by an increase in plasma leptin levels, indicating that leptin may be regulated at transcriptional level. The increase of leptin and UCP mRNA abundance, and leptinemia we report here were not related to plasma glucose or insulin levels. In conclusion, the exposure of broiler chickens to extreme ambient temperatures (cold and heat) increases hepatic leptin and muscle UCP gene expression.

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“…Notably, the amino acid sequence of avian leptin differs from known vertebrate leptins. Earlier studies detected leptin-like immunoreactivity in avian tissues (Kochan et al, 2006, Neglia et al, 2008 or in circulation (Dridi et al, 2000b;Quillfeldt et al, 2009) by using leptin antibodies against mammalian leptin; however, the amino acid sequence of authentic avian leptin indicates an immunogen that maybe inappropriate for detecting avian leptin. Further experiments should demonstrate existence of Ohkubo: Leptin Research in Birds the leptin protein in avian tissues using a specific antibody against bird leptin.…”

Section: Authentic Leptin In Birdsmentioning

confidence: 99%

Recent Progress in Avian Leptin Research

Ohkubo

2014

J. Poult. Sci.

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Because the evidence on existence of the genuine avian leptin gene has been revealed in several birds, debate about the existence of avian leptin will finally stop. Over the past 15 years, many researchers struggled to find avian leptin as well as to determine physiological roles of leptin in birds. During these processes, orthologue of leptin receptor (LEPR) has been identified in several birds and of which function was also deeply investigated.This review summarizes over a decade of research on avian leptin to integrate knowledge toward future advance of this field after finding the authentic leptin in bird.

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A chicken leptin-specific radioimmunoassay

Cited by 32 publications

References 23 publications

Circulating leptin levels do not reflect the amount of body fat in the dunlin Calidris alpina during migration

Circulating leptin levels do not reflect the amount of body fat in the dunlin Calidris alpina during migration

Acute cold‐ and chronic heat‐exposure upregulate hepatic leptin and muscle uncoupling protein (UCP) gene expression in broiler chickens

Recent Progress in Avian Leptin Research

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