A Chemically Synthesized Capture Agent Enables the Selective, Sensitive, and Robust Electrochemical Detection of Anthrax Protective Antigen

Farrow, Blake; Hong, Sung A; Romero, Errika C.; Lai, Bert; Coppock, Matthew B.; Deyle, Kaycie M.; Finch, Amethist S.; Stratis‐Cullum, Dimitra N.; Agnew, Heather D.; Yang, Sung; Heath, James R.

doi:10.1021/nn404296k

Cited by 56 publications

(41 citation statements)

References 43 publications

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“…In the case of bacterial sensing, two classes of biosensors have been developed: (i) those which require sample processing to achieve bacterial disruption or lysis in order to liberate the target bacterial component and (ii) processing-free systems which target whole bacteria. In the first category, biosensors detect bacterial components such as DNA (19,20), RNA (e.g., rRNA) (21,22), intracellular proteins such as enzymes (23), and secreted exotoxins (24). The major disadvantage of these systems is the requirement for sample processing and extra reagents, which increases the time and cost of these tests.…”

Section: Biosensors For Detection Of Bacteriamentioning

confidence: 99%

Biosensors for Whole-Cell Bacterial Detection

et al. 2014

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SUMMARY Bacterial pathogens are important targets for detection and identification in medicine, food safety, public health, and security. Bacterial infection is a common cause of morbidity and mortality worldwide. In spite of the availability of antibiotics, these infections are often misdiagnosed or there is an unacceptable delay in diagnosis. Current methods of bacterial detection rely upon laboratory-based techniques such as cell culture, microscopic analysis, and biochemical assays. These procedures are time-consuming and costly and require specialist equipment and trained users. Portable stand-alone biosensors can facilitate rapid detection and diagnosis at the point of care. Biosensors will be particularly useful where a clear diagnosis informs treatment, in critical illness (e.g., meningitis) or to prevent further disease spread (e.g., in case of food-borne pathogens or sexually transmitted diseases). Detection of bacteria is also becoming increasingly important in antibioterrorism measures (e.g., anthrax detection). In this review, we discuss recent progress in the use of biosensors for the detection of whole bacterial cells for sensitive and earlier identification of bacteria without the need for sample processing. There is a particular focus on electrochemical biosensors, especially impedance-based systems, as these present key advantages in terms of ease of miniaturization, lack of reagents, sensitivity, and low cost.

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Section: Biosensors For Detection Of Bacteriamentioning

confidence: 99%

Biosensors for Whole-Cell Bacterial Detection

et al. 2014

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“…Most of the bio- and nanomaterial-based pathogen sensors developed so far rely on antigen–antibody interaction or sequence-specific nucleic acid detection, and employ optical, electrochemical, piezoelectric, or mass-based transduction to achieve good detection limit and high specificity. 6−15 …”

Section: Introductionmentioning

confidence: 99%

Nanopore Biosensor for Label-Free and Real-Time Detection of Anthrax Lethal Factor

Wang

Yang

Zhou

et al. 2014

ACS Appl. Mater. Interfaces

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We report a label-free real-time nanopore sensing method for the detection of anthrax lethal factor, a component of the anthrax toxin, by using a complementary single-stranded DNA as a molecular probe. The method is rapid and sensitive: sub-nanomolar concentrations of the target anthrax lethal factor DNA could be detected in ∼1 min. Further, our method is selective, which can differentiate the target DNA from other single-stranded DNA molecules at the single-base resolution. This sequence-specific detection approach should find useful application in the development of nanopore sensors for the detection of other pathogens.

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“…The fabrication of electrodes implementing AuNPs functionalized with antibodies has considerably improved the selectivity and sensitivity of different electrochemical methods. An electrochemical ELISA platform for the sensitive detection of anthrax protective antigen (PA) utilized nanostructured gold electrodes coated with a binding peptide for the specific capture of PA [41]. The assay exhibits a limit of detection of less than 3 pM, when tested with serum samples.…”

Section: Nanomaterials For Immunoassaysmentioning

confidence: 99%

Recent Developments in Antibody-Based Assays for the Detection of Bacterial Toxins

Zhu

Dietrich

Didier

et al. 2014

Toxins

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Considering the urgent demand for rapid and accurate determination of bacterial toxins and the recent promising developments in nanotechnology and microfluidics, this review summarizes new achievements of the past five years. Firstly, bacterial toxins will be categorized according to their antibody binding properties into low and high molecular weight compounds. Secondly, the types of antibodies and new techniques for producing antibodies are discussed, including poly- and mono-clonal antibodies, single-chain variable fragments (scFv), as well as heavy-chain and recombinant antibodies. Thirdly, the use of different nanomaterials, such as gold nanoparticles (AuNPs), magnetic nanoparticles (MNPs), quantum dots (QDs) and carbon nanomaterials (graphene and carbon nanotube), for labeling antibodies and toxins or for readout techniques will be summarized. Fourthly, microscale analysis or minimized devices, for example microfluidics or lab-on-a-chip (LOC), which have attracted increasing attention in combination with immunoassays for the robust detection or point-of-care testing (POCT), will be reviewed. Finally, some new materials and analytical strategies, which might be promising for analyzing toxins in the near future, will be shortly introduced.

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A Chemically Synthesized Capture Agent Enables the Selective, Sensitive, and Robust Electrochemical Detection of Anthrax Protective Antigen

Cited by 56 publications

References 43 publications

Biosensors for Whole-Cell Bacterial Detection

Biosensors for Whole-Cell Bacterial Detection

Nanopore Biosensor for Label-Free and Real-Time Detection of Anthrax Lethal Factor

Recent Developments in Antibody-Based Assays for the Detection of Bacterial Toxins

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