A chemical assessment and HPLC assay validation of bulk paromomycin sulfate

High-performance liquid chromatographic determination of vertilmicin in rat plasma using sensitive fluorometric derivatization

“…Regression plot of AUC 0−∞ of vertilmicin vs. dose after intramuscular injection of single doses to rats. [5][6][7] as derivatization reagents to react with aminoglycosides. In this study, FNDB, FMOC-Cl, PITC and OPA were all tested to react with vetilmicin and we found that FMOC-Cl derivates of vertilmicin had advantages of higher sensitivity, less interference and simpler react condition than others.…”

“…1). The analysis of aminoglycosides are usually performed using pre-column [2][3][4][5][6][7][8] and post-column derivatization [9] methods, or other detections such as evaporative light scattering detection [10], pulsed electrochemical detection [11][12][13] and mass spectrometry [12,14,15]. Zhou et al [16] had reported an analytical method for veritlmicin in plasma and 1-fluoro-2,4-dinitrobenzene was used for derivatization.…”

Stability indicating reversed-phase ion-pairing liquid chromatographic determination of vertilmicin sulfate as bulk drug and in injections

“…Like many aminoglycosides, vertilmicin sulfate lacks a suitable chromophore, which is necessary for UV detection. For this reason, the analysis of aminoglycosides is usually performed using pre-column [2][3][4][5][6][7][8], post-column derivatization [9] methods or other detections, such as evaporative light scattering detection [10], pulsed electrochemical detection [11][12][13] or mass spectrometry [12,14]. But sample derivatization can increase the complexity of an HPLC method by requiring additional system components, such as an extra reagent pump, a reaction coil and a mixing.…”

Characterization of paromomycin sulfate by capillary electrophoresis with UV detection after pre-capillary derivatization

“…The most common derivatizing reagents used with the aminoglycoside antibiotics are o-phthaldialdehyde (OPA) with thioglycolic acid (TGA) for UV-detection [16 18], or 2-mercaptoethanol (ME) [19,20] for fluorescence detection. In other applications 2,4-dinitrofluorobenzene (DNFB) [11 13, 21] and reagents such as 2,4,6-trinitrobenzene sulfonic acid (TNBS) [22], dansyl chloride [23], 9-fluorenylmethyl chloroformate (FMOC) [24], and 1-methoxycarb onylindolizine-3,5-dicarbaldehyde [25] have been used.…”