A Chelation-enhanced Fluorescence Assay using Thiourea Capped Carbonaceous Fluorescent Nanoparticles for As (III) Detection in Water Samples

Abstract

Herein, we designed a sensitive and selective "Turn-On" uorescence nanosensor using water-soluble carbonaceous uorescent nanomaterials (CFNs) functionalized with thiourea (CFNs-thiourea) for e cient detection of trace amounts of arsenic (III) in aqueous samples. The CFNs and CFNs-Thiourea were characterized by transmission electron microscopy (TEM), UV-Visible spectroscopy (UV-vis) and Fourier transformed infrared spectroscopy (FTIR). The emission peak intensity of proposed nanosensor at 425 nm was gradually enhanced on arsenite addition in wide detection range (3.3-828.5 µg L -1 ) attributed to the binding of arsenite species with sulfur groups of CFNs-thiourea. The limit of detection (LOD) was 0.48 µg L -1 being much lower than the World Health Organization (WHO) recommended threshold value of 10 µg L -1 . Furthermore, the as prepared thiourea -CFNs exhibited an superb selectivity for As (III) compared various cations and anions such as; NO 3 − , NO

Detection of Arsenic(V) by Fluorescence Sensing Based on Chlorin e6-Copper Ion

“…Currently, there are many techniques available for copper and arsenic detection, such as atomic absorption/emission spectrometry (AAS/AES) [ 10 , 11 , 12 ], atomic fluorescence spectroscopy (AFS) [ 13 , 14 ], X-ray fluorescence spectroscopy (XRF) [ 15 , 16 ], inductively coupled plasma–mass spectrometry (ICP–MS) [ 17 , 18 ], electrochemistry [ 19 , 20 , 21 ], ultraviolet–visible absorption spectrometry (UV–Vis)/colorimetry [ 22 , 23 , 24 , 25 ], and fluorescence spectroscopy [ 26 , 27 , 28 , 29 ], etc. These methods have their characteristics and advantages for testing different environmental samples, but the drawbacks of some methods, such as AAS/AES and ICP–MS, which require expensive and large instruments and specialized operators, limit their applications [ 27 , 30 ]. Compared to conventional UV analytical methods, fluorescence spectroscopy is more sensitive and presents the advantage of a wider linear range, which is a promising tool for rapid and easy tracking of arsenic in environmental monitoring [ 5 ].…”

“…Currently, there are many techniques available for copper and arsenic detection, such as atomic absorption/emission spectrometry (AAS/AES) [10][11][12], atomic fluorescence spectroscopy (AFS) [13,14], X-ray fluorescence spectroscopy (XRF) [15,16], inductively coupled Molecules 2024, 29, 1015 2 of 17 plasma-mass spectrometry (ICP-MS) [17,18], electrochemistry [19][20][21], ultraviolet-visible absorption spectrometry (UV-Vis)/colorimetry [22][23][24][25], and fluorescence spectroscopy [26][27][28][29], etc. These methods have their characteristics and advantages for testing different environmental samples, but the drawbacks of some methods, such as AAS/AES and ICP-MS, which require expensive and large instruments and specialized operators, limit their applications [27,30]. Compared to conventional UV analytical methods, fluorescence spectroscopy is more sensitive and presents the advantage of a wider linear range, which is a promising tool for rapid and easy tracking of arsenic in environmental monitoring [5].…”

Research Progress in Fluorescent Probes for Arsenic Species

“…Arsenic is a toxic and carcinogenic element. Inorganic arsenic is more toxic than organic arsenic, and As 3+ is around 60 times more toxic than As 5+ [ 3 ]. Inorganic arsenic species mainly exist in the form of arsenate in water, such as H 3 AsO 4 , H 3 AsO 3 , H 2 AsO 4 − , H 2 AsO 3 − , AsO 3 3− and AsO 4 3− [ 4 ].…”