A cell-free nanofiber composite scaffold regenerated osteochondral defects in miniature pigs

Filová, Eva; Rampichová, Michala; Litvinec, Andrej; Držík, Milan; Míčková, Andrea; Buzgo, Matěj; Košťáková, Eva Kuželová; Martinová, Lenka; Usvald, Dusan; Prosecká, Eva; Uhlı́k, Jir̆ı́; Motlı́k, Jan; Vajner, Luděk; Amler, Evžen

doi:10.1016/j.ijpharm.2013.02.056

Cited by 57 publications

(56 citation statements)

References 58 publications

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“…The release of GFs from nanofibers was in the order of 1-3 weeks. 29 Implanted scaffolds without the GF were incubated in PBS (pH 7.4) for 12 hours.…”

Section: Methodsmentioning

confidence: 99%

Abdominal closure reinforcement by using polypropylene mesh functionalized with poly-Ԑ-caprolactone nanofibers and growth factors for prevention of incisional hernia formation

Plencner¹,

East²,

Tonar³

et al. 2014

IJN

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Incisional hernia affects up to 20% of patients after abdominal surgery. Unlike other types of hernia, its prognosis is poor, and patients suffer from recurrence within 10 years of the operation. Currently used hernia-repair meshes do not guarantee success, but only extend the recurrence-free period by about 5 years. Most of them are nonresorbable, and these implants can lead to many complications that are in some cases life-threatening. Electrospun nanofibers of various polymers have been used as tissue scaffolds and have been explored extensively in the last decade, due to their low cost and good biocompatibility. Their architecture mimics the natural extracellular matrix. We tested a biodegradable polyester poly-ε-caprolactone in the form of nanofibers as a scaffold for fascia healing in an abdominal closure-reinforcement model for prevention of incisional hernia formation. Both in vitro tests and an experiment on a rabbit model showed promising results.

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“…The release of GFs from nanofibers was in the order of 1-3 weeks. 29 Implanted scaffolds without the GF were incubated in PBS (pH 7.4) for 12 hours.…”

Section: Methodsmentioning

confidence: 99%

Abdominal closure reinforcement by using polypropylene mesh functionalized with poly-Ԑ-caprolactone nanofibers and growth factors for prevention of incisional hernia formation

Plencner¹,

East²,

Tonar³

et al. 2014

IJN

Self Cite

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“…In this regard, different strategies could therefore be devised for increasing the survival of the cells within a fibrin glue or any other gel or scaffold materials. On the other hand, we believe that there is still some room for improvement of cell-free scaffolds such as by the use of materials with different porosity or composition or by delivering biological stimuli from within the scaffold or administered separately [51][52][53][54]. Another important question is the effectiveness of the current approach in repairing large defects, as could be encountered in the clinical setting.…”

Section: Biomechanical Analysismentioning

confidence: 99%

Osteochondral Repair by a Novel Interconnecting Collagen–Hydroxyapatite Substitute: A Large-Animal Study

SosioCorrado

Giancamillo

DepontiDaniela

et al. 2015

Tissue Engineering Part A

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A novel three-dimensional bicomponent substitute made of collagen type I and hydroxyapatite was tested for the repair of osteochondral lesions in a swine model. This scaffold was assembled by a newly developed method that guarantees the strict integration between the organic and the inorganic parts, mimicking the biological tissue between the chondral and the osseous phase. Thirty-six osteochondral lesions were created in the trochlea of six pigs; in each pig, two lesions were treated with scaffolds seeded with autologous chondrocytes (cell+group), two lesions were treated with unseeded scaffolds (cell- group), and the two remaining lesions were left untreated (untreated group). After 3 months, the animals were sacrificed and the newly formed tissue was analyzed to evaluate the degree of maturation. The International Cartilage Repair Society (ICRS) macroscopic assessment showed significantly higher scores in the cell- and untreated groups when compared with the cell+ group. Histological evaluation showed the presence of repaired tissue, with fibroblast-like and hyaline-like areas in all groups; however, with respect to the other groups, the cell- group showed significantly higher values in the ICRS II histological scores for "cell morphology" and for the "surface/superficial assessment." While the scaffold seeded with autologous chondrocytes promoted the formation of a reparative tissue with high cellularity but low glycosaminoglycans (GAG) production, on the contrary, the reparative tissue observed with the unseeded scaffold presented lower cellularity but higher and uniform GAG distribution. Finally, in the lesions treated with scaffolds, the immunohistochemical analysis showed the presence of collagen type II in the peripheral part of the defect, indicating tissue maturation due to the migration of local cells from the surroundings. This study showed that the novel osteochondral scaffold was easy to handle for surgical implantation and was stable within the site of lesion; at the end of the experimental time, all implants were well integrated with the surrounding tissue and no signs of synovitis were observed. The quality of the reparative tissue seemed to be superior for the lesions treated with the unseeded scaffolds, indicating the promising potential of this novel biomaterial for use in a one-stage procedure for osteochondral repair.

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“…To promote regeneration of the tracheal epithelium without a cell source, gel scaffold or growth factor was impregnated into gelatin hydrogel for tracheal defect repair [14,28,29]. Recently, collagen has been used for osteochondral regeneration [30][31][32], and in the present study, PC-NF with or without hUCS showed good cartilage regeneration. Based on the concept that a combination of bioactive growth factors is likely necessary for cartilage repair, we used PC-NF coated with hUCS, which has many growth factors.…”

Section: Discussionmentioning

confidence: 57%

Tracheal regeneration using polycaprolactone/collagen-nanofiber coated with umbilical cord serum after partial resection

Jang¹,

Jang

Cho

et al. 2014

International Journal of Pediatric Otorhinolaryngology

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A cell-free nanofiber composite scaffold regenerated osteochondral defects in miniature pigs

Cited by 57 publications

References 58 publications

Abdominal closure reinforcement by using polypropylene mesh functionalized with poly-Ԑ-caprolactone nanofibers and growth factors for prevention of incisional hernia formation

Abdominal closure reinforcement by using polypropylene mesh functionalized with poly-Ԑ-caprolactone nanofibers and growth factors for prevention of incisional hernia formation

Osteochondral Repair by a Novel Interconnecting Collagen–Hydroxyapatite Substitute: A Large-Animal Study

Tracheal regeneration using polycaprolactone/collagen-nanofiber coated with umbilical cord serum after partial resection

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