“…For the proof of concept, RAW264.7 cells, a kind of mouse macrophage-like cell line with similar functions to primary macrophage cells, were used here as the cell carriers. RAW264.7 cells are often used as cellular vehicles by other investigators [5,[18][19][20] and also employed as the model of tumorassociated macrophages [20]. Then, for the first time, doxorubicin (DOX) loaded RAW264.7 cell delivery system was constructed and related studies in vitro and in vivo including its tumor homing ability, anti-cancer efficacy and toxicity to main organs in a mouse model of lung metastasis of breast cancer, were conducted.…”