A Cell Culture Substrate with Biologically Relevant Size-Scale Topography and Compliance of the Basement Membrane

Garland, Shaun; McKee, Clayton T.; Chang, Yow Ren; Raghunathan, Vijay Krishna; Russell, Paul; Murphy, Christopher J.

doi:10.1021/la403590v

Cited by 20 publications

(15 citation statements)

References 36 publications

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“…Prominent examples are surfaces comprising micro-to-nanoscale post arrays which have been used to study cellular traction forces [122]. Future studies shall systematically study the effect of combination of the topographical parameters with the physico-mechanical ones on cell shape and functions [123].…”

Section: Other Issuesmentioning

confidence: 99%

Controlling the morphology and outgrowth of nerve and neuroglial cells: The effect of surface topography

2017

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There is increasing evidence that physical cues, such as topography, can have a significant impact on the neural cell functions. With the aid of micro-and nanofabrication techniques, new types of cell culture platforms are developed and the effect of surface topography on the cells has been studied. The present review article aims at reviewing the existing body of literature reporting on the use of various topographies to study and control the morphology and functions of cells from nervous tissue, i.e. the neuronal and the neuroglial cells. The cell responses-from phenomenology to investigation of the underlying mechanisms- on the different topographies, including both deterministic and random ones, are summarized.

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Section: Other Issuesmentioning

confidence: 99%

Controlling the morphology and outgrowth of nerve and neuroglial cells: The effect of surface topography

2017

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“…Our initial imaging and unbiased proteomic studies suggest significant changes in the BM of the TM in glaucoma which is beyond age-related changes (Bhattacharya, 2015). The BM environment affects the isolated TM cell stiffness (Garland et al, 2014). Changes in the BM have been also found in retinal vasculature, vitreous, and lens in other ocular diseases (Uechi et al, 2014; Halfter et al, 2015).…”

Section: Basement Membranes and Their Componentsmentioning

confidence: 99%

Segmental outflow of aqueous humor in mouse and human

Carreon

Edwards

Wang

et al. 2017

Experimental Eye Research

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The main and only modifiable risk factor in glaucoma, the group of usually late onset progressive and irreversible blinding optic neuropathies, is elevated intraocular pressure (IOP). The increase in IOP is due to impeded aqueous humor (AH) outflow through the conventional pathway. The aberrant increased resistance at the trabecular meshwork (TM), the filter-like region in the anterior eye chamber is the major contributory factor in causing the impeded outflow. In normal as well as in glaucoma eyes the regions of the TM are divided into areas of high and low flow. The collector channels and distal outflow regions are now increasingly being recognized as potential players in contributing to impede AH outflow. Structural and molecular make-up contributing to the segmental blockage to outflow is likely to provide greater insight. Establishing segmental blockage to outflow in model systems of glaucoma such as the mouse in parallel to human eyes will expand our repertoire of tools for investigation. Further study into this area of interest has the potential to ultimately lead to the development of new therapeutics focused on lowering IOP by targeting the various components of segmental blockage of outflow in the TM and in the distal outflow region.

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“…Key to this is that nanogroove surfaces may promote cellular polarization as well as promote aligned self-assembly of ECM components with motifs critical in cell adhesion such as fibronectin and vitronectin. Indeed, the elongated cellular morphology and alignment induced by grooved substrates may resemble the natural state of tissue resident cell populations in vivo, and studies indicate that most, if not all, cells, notably fibroblasts (Dalby et al, 2003;Garland et al, 2014), osteoblasts (Lenhert et al, 2005;Wu and Wang, 2013), neurons (Yim et al, 2007;Tonazzini et al, 2014), and MSCs (Dalby et al, 2006;McMurray et al, 2013), undergo significant morphological and functional responses to anisotropic topographies.…”

Section: Anisotropic Topography and Cellular Function Anisotropic Biomentioning

confidence: 99%

Sensing the Difference: The Influence of Anisotropic Cues on Cell Behavior

et al. 2015

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From tissue morphogenesis to homeostasis, cells continuously experience and respond to physical, chemical, and biological cues commonly presented in gradients. In this article, we focus our discussion on the importance of nano/micro topographic cues on cell activity, and the role of anisotropic milieus play on cell behavior, mostly adhesion and migration. We present the need to study physiological gradients in vitro. To do this, we review different cell migration mechanisms and how adherent cells react to the presence of complex tissue-like environments and cell-surface stimulation in 2D and 3D (e.g., ventral/dorsal anisotropy).

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A Cell Culture Substrate with Biologically Relevant Size-Scale Topography and Compliance of the Basement Membrane

Cited by 20 publications

References 36 publications

Controlling the morphology and outgrowth of nerve and neuroglial cells: The effect of surface topography

Controlling the morphology and outgrowth of nerve and neuroglial cells: The effect of surface topography

Segmental outflow of aqueous humor in mouse and human

Sensing the Difference: The Influence of Anisotropic Cues on Cell Behavior

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