A cell-compatible PEO–PPO–PEO (Pluronic®)-based hydrogel stabilized through secondary structures

Peng, Sydney; Lin, Ji-Yu; Cheng, Ming‐Huei; Wu, Chia‐Chen; Chu, I‐Ming

doi:10.1016/j.msec.2016.06.091

Cited by 21 publications

(14 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among them, thermo-sensitive in situ gels, composed of cells, anti-inflammatory drugs and growth factors, which could be injected into inflammation sites as solutions and converted to gels rapidly inside the body, has become one of the most promising hydrogels for tissue engineering, especially in bone regeneration [7]. Pluronic F127, a FDA approved synthetic polymer of poly (propylene oxide) (PPO) blocks flanked by poly (ethylene oxide) (PEO) blocks, has been widely used in thermo-sensitive in situ gels [5,8]. The thermos-reversible property makes Pluronic F127 possible to convert its solution state to the gelation state when reaching the critical gelation temperature.…”

Section: Introductionmentioning

confidence: 99%

ECM based injectable thermo-sensitive hydrogel on the recovery of injured cartilage induced by osteoarthritis

Cao

Han

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

View full text Add to dashboard Cite

Intra-articular injection of anti-inflammatory drugs can be a promising strategy for recovery of injured articular cartilage. We prepared a series of injectable thermo-sensitive composite hydrogels, composed of Pluronic F127, glycosaminoglycan (GAG) and bone morphogenetic protein (BMP-2), which was designed to mimic extracellular matrix (ECM). The rheological properties and dissolution rate of composite hydrogels containing chondroitin sulfate or with different hyaluronic acid molecular mass (10k, 90k, 800k) were investigated. Meanwhile, bovine serum albumin (BSA) or FITC-BSA was chosen as model drug loaded into PF/GAG hydrogels to study their sustained release behavior in vitro. The results showed that hydrogels could maintain shapes for more than 16 days and the release rate of BSA in PF/GAG composite gels was much slower than in PF127 gels, due to the affinity between BSA and GAG. Furthermore, increasing the molecular weight of hyaluronic acid correspondingly increased hydrogel dissolution rate and BSA release in the hydrogels. Subsequently, MTT experiments were performed to investigate the toxicity of the hydrogels on mouse pre-osteoblast cell MC3T3-E1. In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).

show abstract

Section: Introductionmentioning

confidence: 99%

ECM based injectable thermo-sensitive hydrogel on the recovery of injured cartilage induced by osteoarthritis

Cao

Han

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

View full text Add to dashboard Cite

show abstract

“…The presence of biodegradable components along the polymeric backbone would allow the copolymer components to degrade into smaller fragments and eventually excreted from the body. Research have reported the use of different types of thermoresponsive polymer systems such as Pluronic F127 hydrogels which consists of triblock copolymers of poly(ethylene glycol), poly(propylene glycol), and poly(ethylene glycol) blocks (PEG–PPG–PEG) . However, this triblock copolymer system is non‐biodegradable.…”

Section: Introductionmentioning

confidence: 99%

New Poly[(R )-3-hydroxybutyrate-co -4-hydroxybutyrate] (P3HB4HB)-Based Thermogels

Wee

Liow

Liu

et al. 2017

Macromol. Chem. Phys.

View full text Add to dashboard Cite

Poly[(R)‐3‐hydroxybutyrate] (P3HB) is a potential candidate for biomaterials due to its biocompatibility and biodegradability. However, P3HB needs to have tunable hydrophobicity, modification through chemical functionalization and the right hydrolytic stability to increase their potential for water‐based biomedical applications such as using them as in situ forming gels for drug delivery. This work focuses on using a copolymer, poly[(R)‐3‐hydroxybutyrate‐co‐4‐hydroxybutyrate] (P3HB4HB) in a thermogelling multiblock system with polypropylene glycol and polyethylene glycol to study the effect of the hydrophobic P3HB4HB on gelation properties, degradation, and drug release rates with reference to P3HB. Thermogels containing P3HB4HB segments show lower critical micellization concentration values in a range from 3 × 10−4 to 1.08 × 10−3 g mL−1 and lower critical gelation concentration values ranging from 2 to 6 wt% than that of gels containing P3HB. Furthermore, gels containing P3HB4HB degrade at a slower rate than the gels containing P3HB. Drug release studies of 5 µg mL−1 of doxorubicin show that gels containing P3HB4HB exhibit sustained release although the release rates are faster than gels containing P3HB. However, this can be modified by varying the concentration of the gels used. Process optimization of purifying the starting material is one important factor before the synthesis of these biomaterials.

show abstract

“…Furthermore, when compared to previously reported dual hydrogel systems based on Pluronic F127 (another synthetic polymer showing reverse thermal gelation for polymer concentration above 20% w / v ), [19] PIC hydrogel offers the advantage of undergoing gelation with a polymer concentration 40 times lower. The high concentration of Pluronic F127 has been proved to be cytotoxic to multiple cells lines [18,39,40,41] and, as a consequence, Pluronic F127 has been used in dual hydrogel systems only as fugitive material [19]. Indeed, the high polymer concentration used in the case of Pluronic F127 allows to attain a higher printing resolution, but to the detriment of cell compatibility.…”

Section: Resultsmentioning

confidence: 99%

3D Printing of Thermoresponsive Polyisocyanide (PIC) Hydrogels as Bioink and Fugitive Material for Tissue Engineering

et al. 2018

View full text Add to dashboard Cite

Despite the rapid and great developments in the field of 3D hydrogel printing, a major ongoing challenge is represented by the development of new processable materials that can be effectively used for bioink formulation. In this work, we present an approach to 3D deposit, a new class of fully-synthetic, biocompatible PolyIsoCyanide (PIC) hydrogels that exhibit a reverse gelation temperature close to physiological conditions (37 °C). Being fully-synthetic, PIC hydrogels are particularly attractive for tissue engineering, as their properties—such as hydrogel stiffness, polymer solubility, and gelation kinetics—can be precisely tailored according to process requirements. Here, for the first time, we demonstrate the feasibility of both 3D printing PIC hydrogels and of creating dual PIC-Gelatin MethAcrylate (GelMA) hydrogel systems. Furthermore, we propose the use of PIC as fugitive hydrogel to template structures within GelMA hydrogels. The presented approach represents a robust and valid alternative to other commercial thermosensitive systems—such as those based on Pluronic F127—for the fabrication of 3D hydrogels through additive manufacturing technologies to be used as advanced platforms in tissue engineering.

show abstract

A cell-compatible PEO–PPO–PEO (Pluronic®)-based hydrogel stabilized through secondary structures

Cited by 21 publications

References 44 publications

ECM based injectable thermo-sensitive hydrogel on the recovery of injured cartilage induced by osteoarthritis

ECM based injectable thermo-sensitive hydrogel on the recovery of injured cartilage induced by osteoarthritis

New Poly[(R )-3-hydroxybutyrate-co -4-hydroxybutyrate] (P3HB4HB)-Based Thermogels

3D Printing of Thermoresponsive Polyisocyanide (PIC) Hydrogels as Bioink and Fugitive Material for Tissue Engineering

Contact Info

Product

Resources

About