A catalogue of genes in the cardiovascular system as identified by expressed sequence tags.

Abstract

The heart, which is composed of all the cellular components of the circulatory system, is a representative organ for obtaining genes expressed in the cardiovascular system in normal and disease states. We used partial sequences of cDNA clones, or expressed sequence tags, to identify and tag genes expressed in this organ. More than 3500 partial sequences representing > 3000 cDNA clones have been obtained from either the 5' or 3' end of inserts derived from human heart cDNA libraries. Of 3132 cDNA clones analyzed by sequence similarity searching against the GenBank/EMBL data bases, 1485 (47.4%) were found to represent additional, previously undiscovered genes, whereas 267 clones were matched to human brain expressed sequence tags. Clones matching to known genes were catalogued according to their putative structural and cellular functions. cDNA probes from reverse-transcribed mRNAs of fetal and adult hearts were used to study differential expression of selected clones in cardiac development. Cataloguing genes expressed in the heart may provide insight into the genes involved in health and cardiovascular disease.

Gene expression profiling in human insulinoma tissue: genes involved in the insulin secretion pathway and cloning of novel full-length cDNAs.

“…The data of GO function assignments indicated that the genes involved in nucleic acid binding and in signal transduction were strongly expressed in insulinoma tissue; this was largely in agreement with previous studies pertaining to gene expression profilings in other human tissues (Liew et al 1994, Mao et al 1998, Hu et al 2000, Endocrine-Related Cancer (2004 www.endocrinology.org Kaestner et al 2003). The study of Hu et al (2000) also showed that genes participating in signaling/cell communication were expressed abundantly in the HPA axis.…”

Identification of 4370 expressed sequence tags from a 3'-end-specific cDNA library of human skeletal muscle by DNA sequencing and filter hybridization.

“…The catalog of transcripts from human skeletal muscle was compared with other EST catalogs such as heart (Liew et al 1994), fetal brain (Adams et al 1993b), hippocampus (Adams et al 1993b), pancreatic islet cells (Takeda et al 1993), and HepG2 cells (Okubo et al 1992). The comparison is quite interesting and indicative of major differences; however, it should be taken into account that in all these projects the cDNA libraries were produced by a variety of methods, often aiming at different goals.…”

“…Several laboratories have recently produced many thousands ESTs from different human tissues and cell lines (Adams et al 1991(Adams et al , 1992(Adams et al , 1993aGieser and Swaroop 1992;Okubo et al 1992;Sikela and Auffrey 1993;Takeda et al 1993;Liew et al 1994;Frigerio et al 1995) and from other organisms (Waterston et al 1992;H6-fte et al 1993;Wan Kim et al 1993).…”

Identification of genes expressed in human CD34+hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning

“…Sequencing of cDNA libraries to generate large numbers of partial cDNA sequences [or expressed sequence tags (ESTs)], combined with bioinformatics, has been shown to be a useful way to evaluate the gene expression profile in a given tissue, to compare transcription levels of many genes between different tissues or cells, and to identify novel genes (12)(13)(14)(15)(16)(17)(18). Characterization of the gene expression patterns in CD34 cells helps to understand the molecular basis of the function and the developmental regulation of these cells as well as the pathogenesis of hematological diseases deriving from the same cells.…”