A calibration strategy for LA-ICP-MS using isotope dilution for solid reference materials

Abstract

An isotope dilution method for the quantitative analysis of trace elements in biological samples and for the characterization of reference materials for the analysis of biological samples by laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-ID-MS) has been tested. Polymeric thin layers with 5-10 mm thickness containing the ID-spike and the correction standard were produced by knife application onto polyester substrates. For the ID experiments 204 Pb enriched lead was used, and bismuth served as the internal standard. The layers showed good homogeneity of the layer thickness and the distribution of added elements. For the testing of the ID method, a synthetic sample with lead and a lead containing polymer certified reference material (BAM H010) have been used. Mass fractions for lead calculated after single and double isotope dilution experiments in the test samples showed a deviation of less than 1% from the certified values.

Laser Ablation–Inductively Coupled Plasma–Mass Spectrometry Imaging in Biology

“…159 Thieleke and Vogt quantified Pb in biological samples and reference materials using isotopically enriched spiked polymers. 160 A sandwich-like structure had three 5−10 μm layers containing a penetration marker (Li), enriched isotope ( 204 Pb), and a correction marker (Bi), and the sample (Pb) was mounted onto a polyester substrate (Figure 13).…”

“…Feng et al deposited and dried isotopically enriched solutions directly on the tissue using a hydrophobic containment barrier for the quantification of Fe, Cu, and Zn in Alzheimer models of murine brains . Thieleke and Vogt quantified Pb in biological samples and reference materials using isotopically enriched spiked polymers . A sandwich-like structure had three 5–10 μm layers containing a penetration marker (Li), enriched isotope ( 204 Pb), and a correction marker (Bi), and the sample (Pb) was mounted onto a polyester substrate (Figure ).…”

Quantitative aspects of inductively coupled plasma mass spectrometry

“…The accuracy of this approach was verified while analysing several elements (Cr, Fe, Cu, Zn, Sr, Cd and Pb) in selected CRMs [74]. Another sound example was provided by Thieleke & Vogt [75]. In their work, 204 Pb-enriched lead was used for performing ID-MS calibration, while bismuth served as IS.…”

“…In order to assure high accuracy of the results, a standard sample containing lead as well as a lead-containing polymer CRM (BAM H010) has been used. The mass fractions for lead, calculated after single and double ID experiments in the test samples, showed a deviation of less than 1% (relative to the certified values) [75]. …”

“…The mass fraction values of total Hg and MeHg, determined in this study, were then used by the International Atomic Energy Agency (IAEA) Environment Laboratories for characterization of the IAEA 461 and IAEA 470 certified reference materials [39]. Although the ID-MS has proved to be unvanquishable in many applications, unfortunately many elements are monoisotopic ( 9 Be, 23 Na, 27 Al, 45 Sc, 55 Mn, 75 As, 89 Y, 103 Rh, 127 I, 133 Cs, 141 Pr, 159 Tb, 165 Ho, 169 Tm, 197 Au, and 232 Th), which makes ID-ICP-MS useless in analysis of these elements.…”