A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria

Isolation and characterization of mutants of Pseudomonas maltophila PM-4 altered in chitinolytic activity and antagonistic activity against root rot pathogens of clusterbean (Cyamopsis tetragonoloba)

“…Colloidal chitin was prepared as described by Lingappa and Lockhood 16 . Mutagenesis of P. maltophila: Random Tn5 mutagenesis of P. maltophila PM-4 was carried out using E. coli S17-1(pSUP1021) following the procedure as described earlier 17,18 .…”

Aerogenic vaccination with a Burkholderia mallei auxotroph protects against aerosol-initiated glanders in mice

“…Escherichia coli TOP10 (Invitrogen, Carlsbad, CA) was used for general cloning and blue/white screening, and E. coli S17-1 [17] was used as a mobilizing strain for constructing mutants. The B. mallei mutant strains used in this study were derivatives of ATCC 23344, a highly pathogenic clinical isolate and type strain of the species [18][19][20].…”

Promoter tagging with a promoterless ipt gene leads to cytokinin‐induced phenotypic variability in transgenic tobacco plants: implications of gene dosage effects

“…The Escherichia coil strains used were HB101 (Boyer and Roulland Dussoix, 1969) and SM10 (Simon et al, 1983). For plant transformation a novel Agrobacterium tumefaciens strain was constructed, that harbors the non-T-DNA portion of Ti plasmid pTiBo542 of the hypervirulent A. tumefaciens strain A281 (Hood et al, 1986).…”