A branched electrode based electrochemical platform: towards new label-free and reagentless simultaneous detection of two biomarkers

Abstract

A branched electrode platform was proposed for label-free and reagentless simultaneous tumor markers detection based on different redox substrates.

Recent advances in electrochemical biosensors based on graphene two-dimensional nanomaterials

“…Moreover, the electron mediation of MB between electrodes allows high signal amplification. A branched label-free electrochemical immune-platform for the simultaneous detection of two biomarkers was introduced using a similar strategy (Kong et al, 2013). MB and Prussian blue (PB) were selected as redox mediators to indicate different antigens.…”

Design of immunoprobes for electrochemical multiplexed tumor marker detection

“…However, the increased levels of tumor markers in human serum are challenging samples to analyze as the protein concentration range spans several orders of magnitude, and tumor markers are generally low-level proteins that are extremely difficult to be detected in these highly complex samples [12,13]. To meet the requirements of monitoring trace tumor markers, many researchers have focused on the detection of a single tumor marker, ignoring the complexity of biological system, especially the human body [14][15][16][17]. Their predictive value is usually limited because most tumor markers are not specific and can sometimes give falsepositive results.…”

Simultaneous quantitative detection of multiple tumor markers with a rapid and sensitive multicolor quantum dots based immunochromatographic test strip

“…Various approaches have been proposed to perform simultaneous detection of multiple tumor markers in the past few years. For example, electrochemical immunoassay (Wilson, 2007;Xu et al, 2014), label-free methods (Lin et al, 2011;Kong et al, 2013), dielectrophoresi assays (Lee et al, 2010;Ramón-Azcón et al, 2011), chemiluminescence alalysis (Zong et al, 2012;Guo et al, 2013), biobarcoded Scanometric assay (Stoeva et al, 2006), spectrometry (Chon et al, 2011;Giesen et al, 2011), fluorescence detection (Tian et al, 2012;Akinfieva et al, 2013), photonic suspension array (Zhao et al, 2009). But all these techniques are not suitable for point-of-care testing because they require large and sophisticated instruments, complex operations, long analysis time and highly skilled personnel to manipulate.…”