A bovine serum albumin and squaraine dye assembly fluorescent probe for pepsin detection

Jiang, Qinan; Chen, Zhe; Huang, Yueyue; Gao, Qiancheng; Luo, Canxia; Mehdi, Muhammad; Xu, Yongqian; Li, Hongjuan; Sun, Shiguo

doi:10.1016/j.microc.2022.108361

Cited by 9 publications

(5 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The newly proposed peptide–dye conjugate distinctly shows the attachment of two squaraine dye molecules at both ends of the peptide terminals. As a result of its far-red fluorescence emission and its favorable interactions with frequently utilized model proteins, including human serum albumin and bovine serum albumin, squaraine dye was selected as the model dye [ 29 , 30 , 31 , 32 ]. The squaraine dye’s chain length plays a pivotal role in its behavior in aqueous environments, influencing aggregate formation.…”

Section: Resultsmentioning

confidence: 99%

Design and Synthesis of Novel Squaraine-Based Fluorescent Probe for Far-Red Detection of Chymotrypsin Enzyme

Gupta,

Balyan,

Mavileti

et al. 2024

Molecules

View full text Add to dashboard Cite

Chymotrypsin, a crucial enzyme in human digestion, catalyzes the breakdown of milk proteins, underscoring its significance in both health diagnostics and dairy quality assurance. Addressing the critical need for rapid, cost-effective detection methods, we introduce a groundbreaking approach utilizing far-red technology and HOMO-Förster resonance energy transfer (FRET). Our novel probe, SQ-122 PC, features a unique molecular design that includes a squaraine dye (SQ), a peptide linker, and SQ moieties synthesized through solid-phase peptide synthesis. Demonstrating a remarkable quenching efficiency of 93.75% in a tailored H2O:DMSO (7:3) solvent system, our probe exhibits absorption and emission properties within the far-red spectrum, with an unprecedented detection limit of 0.130 nM. Importantly, our method offers unparalleled selectivity towards chymotrypsin, ensuring robust and accurate enzyme detection. This pioneering work underscores the immense potential of far-red-based homo-FRET systems in enabling the sensitive and specific detection of chymotrypsin enzyme activity. By bridging the gap between cutting-edge technology and biomedical diagnostics, our findings herald a new era of enzyme sensing, promising transformative advancements in disease diagnosis and dairy quality control.

show abstract

Section: Resultsmentioning

confidence: 99%

Design and Synthesis of Novel Squaraine-Based Fluorescent Probe for Far-Red Detection of Chymotrypsin Enzyme

Gupta,

Balyan,

Mavileti

et al. 2024

Molecules

View full text Add to dashboard Cite

show abstract

“…No. Imaging agents 68 Ga-labeled HSA ( Brunner et al, 2012 ) 68 F-labeled HSA ( Chang et al, 2005 ) 111 In-labeled HSA ( Palmowski et al, 2013 ) Gd-labeled HSA ( Ogan et al, 1987 ) Therapeutic agents Doxorubicin HSA conjugates ( Chen et al, 2023b ) Doxorubicin prodrug ( Yousefpour et al, 2019 ) Pt(II) prodrugs ( Paul et al, 2024 ) Camptothecin prodrug ( Cheng et al, 2021 ) Methotrexate ( Faghfoori et al, 2020 ) Theranostic agents CysCOOH HSA conjugate ( Rong et al, 2015 ) Ce6 HSA conjugate ( Zhang et al, 2020b ) Cyanine albumin conjugate ( Tang et al, 2018 ) Imaging agents SQ-BSA complex ( Jiang et al, 2023 ) Therapeutic agents DOX-HSA prodrug ( Zhang et al, 2020a ) ICG@holo-Tf NAs ( Zhu et al, 2017 ) Theranostic agents SQ-BSA complex ( …”

Section: Protein-drug Nano-conjugatesmentioning

confidence: 99%

Rationalized landscape on protein-based cancer nanomedicine: Recent progress and challenges

Fan,

Iqbal,

et al. 2024

International Journal of Pharmaceutics: X

View full text Add to dashboard Cite

“…There are various methods for detecting albumin, each with its own set of advantages and disadvantages. Immunoassays such as enzyme-linked immunosorbent assay (ELISA) [3], Radio immuno assay (RIA) [4], and aptamers [5] have excellent specificity and sensitivity but require labeling of the analyte, which adds complication. Spectrophotometric approaches rely on light absorption by albumin, although they frequently require particular reagents or dyes.…”

Section: Introductionmentioning

confidence: 99%

Synergistic integration of Ni-metal organic framework/SnS₂ nanocomposite and nickel foam electrode for ultrasensitive and selective electrochemical detection of albumin in simulated human blood serum

Singh,

Aggrawal,

Badhulika

2024

Nanotechnology

View full text Add to dashboard Cite

Albumin is a vital blood protein responsible for transporting metabolites and drugs throughout the body and serves as a potential biomarker for various medical conditions, including inflammatory, cardiovascular, and renal issues. This report details the fabrication of Ni-metal organic framework/SnS2 nanocomposite modified nickel foam electrochemical sensor for highly sensitive and selective non enzymatic detection of albumin in simulated human blood serum samples. Ni-metal organic framework/SnS2 nanocomposite was synthesized using solvothermal technique by combining Ni-MOF with conductive SnS2 leading to the formation of a highly porous material with reduced toxicity and excellent electrical conductivity. Detailed surface morphology and chemical bonding of the Ni-MOF/SnS2 nanocomposite was studied using scanning electron microscopy (SEM), transmission electron microscopy (TEM), FTIR, and Raman analysis. The Ni-MOF/SnS2 nanocomposite coated on Ni foam electrode demonstrated outstanding electrochemical performance, with a low limit of detection (0.44 µM) and high sensitivity (1.3 µA/pM/cm2) throughout a broad linear range (100 pM - 10 mM). The remarkable sensor performance is achieved through the synthesis of a Ni-MOF/SnS2 nanocomposite, enhancing electrocatalytic activity for efficient albumin redox reactions. The enhanced performance can be attributed due to the structural porosity of nickel foam and Ni-metal organic framework, which favours increased surface area for albumin interaction. The presence of SnS2 shows stability in acidic and neutral solutions due to high surface to volume ratio which in turn improves sensitivity of the sensing material. The sensor exhibited commendable selectivity, maintaining its performance even when exposed to potential interfering substances like glucose, ascorbic acid, K+, Na+, uric acid, and urea. The sensor effectively demonstrates its accuracy in detecting albumin in real samples, showcasing substantial recovery percentages of 105.1%, 110.28%, and 91.16%.

show abstract

A bovine serum albumin and squaraine dye assembly fluorescent probe for pepsin detection

Cited by 9 publications

References 41 publications

Design and Synthesis of Novel Squaraine-Based Fluorescent Probe for Far-Red Detection of Chymotrypsin Enzyme

Design and Synthesis of Novel Squaraine-Based Fluorescent Probe for Far-Red Detection of Chymotrypsin Enzyme

Rationalized landscape on protein-based cancer nanomedicine: Recent progress and challenges

Synergistic integration of Ni-metal organic framework/SnS₂ nanocomposite and nickel foam electrode for ultrasensitive and selective electrochemical detection of albumin in simulated human blood serum

Contact Info

Product

Resources

About

A bovine serum albumin and squaraine dye assembly fluorescent probe for pepsin detection

Cited by 9 publications

References 41 publications

Design and Synthesis of Novel Squaraine-Based Fluorescent Probe for Far-Red Detection of Chymotrypsin Enzyme

Design and Synthesis of Novel Squaraine-Based Fluorescent Probe for Far-Red Detection of Chymotrypsin Enzyme

Rationalized landscape on protein-based cancer nanomedicine: Recent progress and challenges

Synergistic integration of Ni-metal organic framework/SnS2 nanocomposite and nickel foam electrode for ultrasensitive and selective electrochemical detection of albumin in simulated human blood serum

Contact Info

Product

Resources

About

Synergistic integration of Ni-metal organic framework/SnS₂ nanocomposite and nickel foam electrode for ultrasensitive and selective electrochemical detection of albumin in simulated human blood serum