A blue catalase screening test for pyuria and haematuria

Thomas, Mair; Baldwin, Gillian

doi:10.1136/jcp.24.4.320

Cited by 3 publications

(1 citation statement)

References 14 publications

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“…Moreover, the new isolate, resulting positive to the hydrolytic activity of PCL and tributyrin, was preliminary morphologically characterized by growth on the LB agar plate, and Gram stain examined under a microscope with a magnification up to 100X (Axiolab E re, Carl Zeiss, Germany). Oxidase and catalase tests were also performed according to standard protocols (Gaby and Hadley, 1957 ; Thomas, 1963 ).…”

Section: Methodsmentioning

confidence: 99%

Insights into the biodegradation of polycaprolactone through genomic analysis of two plastic-degrading Rhodococcus bacteria

Zampolli,

Vezzini,

Brocca

et al. 2024

Front. Microbiol.

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Polycaprolactone (PCL) is an aliphatic polyester often utilized as a model to investigate the biodegradation potential of bacteria and the involved catabolic enzymes. This study aims to characterize PCL biodegradative metabolic potential and correlate it to genomic traits of two plastic-degrading bacteria—Rhodococcus erythropolis D4 strain, a new isolate from plastic-rich organic waste treatment plant, and Rhodococcus opacus R7, known for its relevant biodegradative potential on polyethylene and similar compounds. After preliminary screening for bacteria capable of hydrolyzing tributyrin and PCL, the biodegradation of PCL was evaluated in R. erythropolis D4 and R. opacus R7 by measuring their growth and the release of PCL catabolism products up to 42 days. After 7 days, an increase of at least one order of magnitude of cell number was observed. GC-MS analyses of 28-day culture supernatants showed an increase in carboxylic acids in both Rhodococcus cultures. Furthermore, hydrolytic activity (~5 U mg−1) on short/medium-chain p-nitrophenyl esters was detected in their supernatant. Finally, a comparative genome analysis was performed between two Rhodococcus strains. A comparison with genes annotated in reference strains revealed hundreds of gene products putatively related to polyester biodegradation. Based on additional predictive analysis of gene products, gene expression was performed on a smaller group of genes, revealing that exposure to PCL elicits the greatest increase in transcription for a single gene in strain R7 and two genes, including that encoding a putative lipase, in strain D4. This work exhibits a multifaceted experimental approach to exploit the broad potential of Rhodococcus strains in the field of plastic biodegradation.

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Section: Methodsmentioning

confidence: 99%