A Blocking Enzyme‐Linked Immunosorbent Assay (ELISA) for Bovine Virus Diarrhoea Virus Serology

Westenbrink, F.; Middel, W.G.J.; Straver, P. J.; Leeuw, Peter W. de

doi:10.1111/j.1439-0450.1986.tb00042.x

Cited by 29 publications

(14 citation statements)

References 14 publications

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“…Absence of BHV 1-specific antibodies in sera was ascertained using a commercial available ELISA (Labease, SmithKline Beecham). BVDV antibodies were determined using a blocking ELISA as described by Westenbrink et al [27].…”

Section: Sentinel Cowsmentioning

confidence: 99%

Dynamics of bovine respiratory syncytial virus infections: a longitudinal epidemiological study in dairy herds

Poel

Kramps²,

Middel³

et al. 1993

Archives of Virology

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To study the epidemiology of respiratory syncytial virus (RSV) infections during the year, the incidences of primary infections and reinfections were monitored by titrating antibodies to bovine RSV (BRSV) in cattle above 2 months of age in 6 dairy herds in the Netherlands. From August 1990 until September 1991, 884 cattle were sampled at one-month intervals. A total of 155 cattle, most under two years of age, had a primary antibody response. Antibody rises were found in 259 cattle of all ages. The highest incidences of BRSV infections were found in one period either in autumn or winter. In other seasons, primary infections were rare, whereas reinfections were not uncommon. In 5 out of the 6 herds, two seronegative sentinel calves were introduced at the end of the winter and none developed specific antibodies before the next winter. The observations strongly suggest that, in spite of regular reinfections, BRSV circulates during spring or summer at a very low level or not at all. Persistent BRSV infection in a number of cows might be a means for the virus to survive during summer, but a steady rate of reinfection of seropositive cows throughout the year at a low level might also maintain a reservoir of infectious virus. This study adds to the knowledge of frequency and timings of primary infections and reinfections of BRSV and it might contribute to the study of these issues of human RSV.

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Section: Sentinel Cowsmentioning

confidence: 99%

Dynamics of bovine respiratory syncytial virus infections: a longitudinal epidemiological study in dairy herds

Poel

Kramps²,

Middel³

et al. 1993

Archives of Virology

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“…Sera were tested in enzyme-linked immunosorbent assays for antibodies directed against glycoprotein gI of pseudorabies virus (21), porcine parvovirus (28), bovine viral diarrhoea virus (29), and hog cholera virus (24). Starting dilutions were 1:5, after which the sera were diluted twofold.…”

Section: Alycoplasma Isolationmentioning

confidence: 99%

Mystery swine disease in the Netherlands: The isolation of Lelystad virus

Wensvoort¹,

Terpstra²,

Pol³

et al. 1991

Veterinary Quarterly

1,189

829

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“…The correlation coefficient existing between the serum neutralization titration and the blocking ELISA-which can be considered very good for two test methods based on different principles (Westenbrink et al, 1986;Zhzng et al, 1990)-indicates that blocking ELISA is suitable for replacing the conventional procedure both in diagnostic work and in control operations of vaccine production.…”

Section: Resultsmentioning

confidence: 99%

Use of ELISA procedures for the detection of Derzsy's disease virus of geese and of antibodies produced against it

Kardi

Szegletes

1996

Avian Pathology

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SUMMARYWe have developed a double antibody sandwich (antigen capture) ELISA for the detection of Derzsy's disease virus (GPV-1) antigen produced in cell cultures. This provided unambiguous quantification of virus whereas evaluation of cytopathic effect was sometimes difficult. A blocking ELISA was developed to quantify anti-GPV-1 antibodies in goose sera. This method was rapid, simple, easily standardized and correlated well with a virus neutralization test.

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A Blocking Enzyme‐Linked Immunosorbent Assay (ELISA) for Bovine Virus Diarrhoea Virus Serology

Cited by 29 publications

References 14 publications

Dynamics of bovine respiratory syncytial virus infections: a longitudinal epidemiological study in dairy herds

Dynamics of bovine respiratory syncytial virus infections: a longitudinal epidemiological study in dairy herds

Mystery swine disease in the Netherlands: The isolation of Lelystad virus

Use of ELISA procedures for the detection of Derzsy's disease virus of geese and of antibodies produced against it

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