A biphasic scaffold design combined with cell sheet technology for simultaneous regeneration of alveolar bone/periodontal ligament complex

Vaquette, Cédryck; Fan, Wei; Xiao, Yin; Hamlet, Stephen; Hutmacher, Dietmar W.; Ivanovski, Sašo

doi:10.1016/j.biomaterials.2012.04.038

Cited by 199 publications

(207 citation statements)

References 45 publications

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“…[29][30][31][32][33] Despite the outlined efforts to orchestrate multitissue formation in vivo, lack of control over the fibrous connective tissue orientation and the integration between ligament and mineralized tissues limit the preclinical and clinical application of multi-tissue regenerative therapies. Conventional scaffold design and manufacturing methods do not allow for compartmentalization of differing tissues and, thus, subsequent re-organization and integration of fibrous and mineralized tissues.…”

Section: Introductionmentioning

confidence: 99%

Image-Based, Fiber Guiding Scaffolds: A Platform for Regenerating Tissue Interfaces

Park

Ríos

Taut

et al. 2014

Tissue Engineering Part C: Methods

100

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Section: Introductionmentioning

confidence: 99%

Image-Based, Fiber Guiding Scaffolds: A Platform for Regenerating Tissue Interfaces

Park

Ríos

Taut

et al. 2014

Tissue Engineering Part C: Methods

100

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“…After 4 min of electrospinning, highly porous PCL melt-electrospun mesh with roughly 8 mm diameter was produced on top of the aluminium foil-covered glass slides placed over the collector. The biphasic scaffold was assembled as previously reported [25,26]. Briefly, 6 mm long FDM scaffold cylinders were punched out using a 4 mm biopsy punch and one side of the cylinder was placed 1 cm from a hot plate heated to 3008C for 4 s and then quickly press-fitted for 10 s onto the pre-cut PCL meshes (4 mm diameter, approx.…”

Section: Biphasic Construct Fabrication Assembly and Osteoblast Seedmentioning

confidence: 99%

Multiphasic construct studied in an ectopic osteochondral defect model

Jeon

Vaquette

Theodoropoulos

et al. 2014

J. R. Soc. Interface.

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In vivo osteochondral defect models predominantly consist of small animals, such as rabbits. Although they have an advantage of low cost and manageability, their joints are smaller and more easily healed compared with larger animals or humans. We hypothesized that osteochondral cores from large animals can be implanted subcutaneously in rats to create an ectopic osteochondral defect model for routine and high-throughput screening of multiphasic scaffold designs and/or tissue-engineered constructs (TECs). Bovine osteochondral plugs with 4 mm diameter osteochondral defect were fitted with novel multiphasic osteochondral grafts composed of chondrocyteseeded alginate gels and osteoblast-seeded polycaprolactone scaffolds, prior to being implanted in rats subcutaneously with bone morphogenic protein-7. After 12 weeks of in vivo implantation, histological and micro-computed tomography analyses demonstrated that TECs are susceptible to mineralization. Additionally, there was limited bone formation in the scaffold. These results suggest that the current model requires optimization to facilitate robust bone regeneration and vascular infiltration into the defect site. Taken together, this study provides a proof-of-concept for a high-throughput osteochondral defect model. With further optimization, the presented hybrid in vivo model may address the growing need for a cost-effective way to screen osteochondral repair strategies before moving to large animal preclinical trials.

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“…This approach has been successfully used to promote periodontal regeneration in a number of small and large animal models (Akizuki et al, 2005;Flores et al, 2008;Ishikawa et al, 2009;Vaquette et al, 2012;Costa et al, 2014).…”

mentioning

confidence: 99%

Decellularized Periodontal Ligament Cell Sheets with Recellularization Potential

et al. 2014

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The periodontal ligament is the key tissue facilitating periodontal regeneration. This study aimed to fabricate decellularized human periodontal ligament cell sheets for subsequent periodontal tissue engineering applications. The decellularization protocol involved the transfer of intact human periodontal ligament cell sheets onto melt electrospun polycaprolactone membranes and subsequent bi-directional perfusion with NH 4 OH/Triton X-100 and DNase solutions. The protocol was shown to remove 92% of DNA content. The structural integrity of the decellularized cell sheets was confirmed by a collagen quantification assay, immunostaining of human collagen type I and fibronectin, and scanning electron microscopy. ELISA was used to demonstrate the presence of residual basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), and hepatocyte growth factor (HGF) in the decellularized cell sheet constructs. The decellularized cell sheets were shown to have the ability to support recellularization by allogenic human periodontal ligament cells. This study describes the fabrication of decellularized periodontal ligament cell sheets that retain an intact extracellular matrix and resident growth factors and can support repopulation by allogenic cells. The decellularized hPDL cell sheet concept has the potential to be utilized in future "off-theshelf " periodontal tissue engineering strategies.

show abstract

A biphasic scaffold design combined with cell sheet technology for simultaneous regeneration of alveolar bone/periodontal ligament complex

Cited by 199 publications

References 45 publications

Image-Based, Fiber Guiding Scaffolds: A Platform for Regenerating Tissue Interfaces

Image-Based, Fiber Guiding Scaffolds: A Platform for Regenerating Tissue Interfaces

Multiphasic construct studied in an ectopic osteochondral defect model

Decellularized Periodontal Ligament Cell Sheets with Recellularization Potential

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