A biosensor for the determination of amylase activity

Zajoncová, Ludmila; Jı́lek, Milan; Beranová, Veronika; Peč, Pavel

doi:10.1016/j.bios.2004.01.006

Cited by 52 publications

(33 citation statements)

References 26 publications

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“…[6][7][8] Mainly, the methods are divided into 2 categories: covalent bonding and entrapping. While the possibility of loss of the immobilized enzyme exists, through leakage or dissolution, it is small for immobilization achieved by covalent bonding [9][10][11] and cross-linking, [12][13][14][15][16][17][18][19][20] and there remains fear of enzyme activity loss due to reagents used in the bonding reaction; there are means for prolonging the duration of enzyme stability, but the enzyme may be damaged during such immobilization processes.…”

Section: Introductionmentioning

confidence: 99%

Preparation of a Cellulose-based Enzyme Membrane Using Ionic Liquid to Lengthen the Duration of Enzyme Stability

et al. 2012

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Section: Introductionmentioning

confidence: 99%

Preparation of a Cellulose-based Enzyme Membrane Using Ionic Liquid to Lengthen the Duration of Enzyme Stability

et al. 2012

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“…In order to analyse the salivary amylase activity continuously, a flow-injection-type biosensor system has already been proposed, using a GD enzymatic membrane (Yamaguchi et al, 2003). Moreover, mutarotase (EC 5.1.3.3) was added to the enzymatic membrane to shorten the reaction time (Zajoncová et al, 2004). However, the enzymatic activity of GD used in the biosensor significantly decreased due to immobilisation.…”

Section: Introductionmentioning

confidence: 99%

Flat-Chip Microanalytical Enzyme Sensor for Salivary Amylase Activity

Yamaguchi

Deguchi

Wakasugi

et al. 2005

Biomed Microdevices

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It is considered that measurement of salivary alpha-amylase activity is a useful tool for evaluating the sympathetic nervous system. The purpose of this research is to demonstrate a new design of a flat-chip microanalytical enzyme sensor (flat-chip sensor) for salivary amylase activity as a Micro-Electro-Mechanical Systems (MEMS), which may be used for wearable analytical systems. To meet this purpose, the biosensor needs to be miniaturized and to possess high-sensitivity. A pre-column and a flat-enzyme electrode were incorporated in a flow cell of volume 25.7 ml. In order to miniaturize the flow cell, two enzymatic membranes containing maltose phosphorylase obtained from Enterococcus hirae (MP membrane) and glucose oxidase and peroxidase (GOD-POD membrane) were immobilised on the same planar surface. As a result, a flat-chip sensor incorporating a flow cell as small as a C battery was produced. The optimum conditions of three parameters of the fabricated flat-chip sensor, the immobilising method of the enzymatic membrane, dropping volume of the mixed enzymatic solution and flow rate of the sample solution, were examined. An analytical system for 0-190 kU/l amylase activity with R(2) of 0.97 was fabricated with a sample volume of 50 microl. This research indicates the possibility of a wearable biosensor for continuous monitoring of salivary amylase activity.

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“…The total amount of polyamines in pea extracts was determined with an amperometric biosensor using immobilised diamine oxidase and polyamine oxidase. A commercial biosensor analyser (M. Jilek Company, Postřelmov, Czech Republic) connected to a PC was used as a potentiostat (Zajoncová et al 2004). Pea amine oxidase and maize polyamine oxidase were purified as described previously (Šebela et al 1998;Federico et al 1989) and the enzymes were immobilised on a cellophane membrane.…”

Section: Methodsmentioning

confidence: 99%

Modulation of polyamine catabolism in pea seedlings by calcium during salinity stress

Piterková¹,

Luhová²,

Zajoncová³

et al. 2012

Plant Prot. Sci.

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Piterková J., Luhová L., Zajoncová L., Šebela M., Petřivalský M. (2012): Modulation of polyamine catabolism in pea seedlings by calcium during salinity stress. Plant Protect. Sci., 48: 53-64.The relation of polyamine catabolism in the response of Pisum sativum to salinity stress was investigated. Pea seedlings were grown in increasing concentrations of Na + or K + or at different concentration ratios of these ions.We studied the effect of Ca 2+ supplementation on plants exposed to salinity stress. The parameters measured in the roots and shoots of pea seedlings included biomass production, levels of Na + , K + , Ca 2+ and polyamines and activity of enzymes of polyamine catabolism: diamine oxidase, aminoaldehyde dehydrogenase and peroxidases. Salinity induced increased polyamine levels and higher activity of enzymes participating in polyamine degradation. Supplementation of Ca 2+ had a positive effect on biomass production and in most cases it stabilised both the polyamine level and the activity of the studied enzymes. Our results confirm the role of aminoaldehyde dehydrogenase and polyamine catabolism in defence mechanisms of pea plants under salinity stress.

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A biosensor for the determination of amylase activity

Cited by 52 publications

References 26 publications

Preparation of a Cellulose-based Enzyme Membrane Using Ionic Liquid to Lengthen the Duration of Enzyme Stability

Preparation of a Cellulose-based Enzyme Membrane Using Ionic Liquid to Lengthen the Duration of Enzyme Stability

Flat-Chip Microanalytical Enzyme Sensor for Salivary Amylase Activity

Modulation of polyamine catabolism in pea seedlings by calcium during salinity stress

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