A Biosensor-Based Quantitative Analysis System of Major Active Ingredients in Lonicera japonica Thunb. Using UPLC-QDa and Chemometric Analysis

Abstract

In the study, a surface plasmon resonance-based (SPR-based) competitive assay was performed to analyze different compounds’ inhibitory activity to TNF-α, an important pro-inflammatory cytokine in the pathogenesis of chronic inflammatory diseases. Moreover, the single mass spectrometry (MS) detection method was coupled with an ultra-high-performance liquid chromatography (UPLC) system for the routine quality control (QC) of a traditional Chinese medicine (TCM). The above quality control strategy was evaluated with Lonicera japonica Thunb. Analytes were firstly separated on a Waters ACQUITYTM UPLC HSS T3 column (2.1 × 50 mm; particle size = 1.8 μm) using a 0.1% formic acid gradient elution, then detected by negative ESI mass spectrometry. The limits of quantification (LOQ) for analytes reached 0.005–0.56 μg/mL. The LOD of the QDa detector was lower than that of the PDA detector, indicating its wider detection range. The QDa detector was also more suitable for the analysis of the complex matrix of TCM. The method showed excellent linearity, with regression coefficients higher than 0.9991. The average recoveries of the investigated analytes were in the range of 98.78–105.13%, with an RSD below 3.91%. The inter-day precision range (n = 3 days) was 2.51–4.54%. Compared to other detectors, this strategy could be widely applied in the quantitative analysis of TCM. In addition, the chemically latent data could be revealed using chemometric analysis. Importantly, this study provides an efficient screening method for small-molecule inhibitors targeting the TNF-α pathway.

Identification and Screening of Natural Neuraminidase Inhibitors from Reduning Injection via One-Step High-Performance Liquid Chromatography-Fraction Collector and UHPLC/Q-TOF-MS

“…e results can be shown in Figure 6. e fractions (3,4,5,6,8,15,18,19,20,21,23,25,27,29,30,31,32,38,39,40,46,47,66,67,68,69,70, and 71) contained peaks which have the same retention time with RDN injection. Finally, the corresponding relationship between fraction number and peak number of the potential active components with NA inhibition is shown in Table 3.…”

“…e reason why the rate was chosen at 6% was that the systematic error was 6% obtained by the calibration curve for screening the NAIs. In total, the inhibitory ratios of 28 fractions which were truly active fractions among the 75 fractions (fractions 3,4,5,6,8,15,18,19,20,21,22,23,25,29,30,31,32,38,39,40,46,47,66,67,68,69, 70, and 71) increased with an increasing concentration, which exhibited obvious relativity between the dose and the effect as shown in Figure 5. ese fractions have been indicated as the potential NAIs.…”

Insights into forsythia honeysuckle (Lianhuaqingwen) capsules: A Chinese herbal medicine repurposed for COVID-19 pandemic

“…Compounds 17-33 originate from honeysuckle, which is often clinically used to fight inflammation( Yang et al, 2019 ). Its main active ingredients are phenolic acids and flavonoids( Peng et al, 2005 ).…”

Discovering the Major Antitussive, Expectorant, and Anti-Inflammatory Bioactive Constituents in Tussilago farfara L. Based on the Spectrum–Effect Relationship Combined with Chemometrics

“…In this paper, the UPLC-QDA method was used to establish the chromatography fingerprint of raw and processed FF and to carry out a similarity analysis (SA) among these samples [21]. Then, principal component analysis (PCA) and hierarchical cluster analysis (HCA) were performed to classify 18 batches of FF, following which 10 representative batches of FF (six batches of raw FF and four batches of processed FF) were screened to study their antitussive, expectorant, and anti-inflammatory effects [22].…”