A biofunctional-modified silk fibroin scaffold with mimic reconstructed extracellular matrix of decellularized pulp/collagen/fibronectin for bone tissue engineering in alveolar bone resorption

Sangkert, Supaporn; Kamonmattayakul, Suttatip; Chai, Wen Lin; Meesane, Jirut

doi:10.1016/j.matlet.2015.12.032

Cited by 26 publications

(17 citation statements)

References 13 publications

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“…The medium was changed every 3 days during culture. OS medium was used for osteoblast differentiation of the MG‐63 cells . MG‐63 cells were cultured in a culture plate for 1, 3, 5, and 7 days.…”

Section: Methodsmentioning

confidence: 99%

Biocomposite foams from poly(lactic acid) and rubber wood sawdust: Mechanical properties, cytotoxicity, and in vitro degradation

Sungsee

Tanrattanakul

2019

J of Applied Polymer Sci

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The objectives of this work were to seek a simple method for preparation of poly(lactic acid) (PLA) foams and evaluate properties of these foams for scaffold application. Using a typical blowing agent and compression molding, biocomposite foams were successfully prepared from a PLA/rubber wood sawdust (PLA/RWS) blend. Selection of RWS for the biocomposites was based on particle size. RWS particles in two size ranges were used: 212–600 μm and ≤75 μm. Alkaline and silane treatments were applied to the RWS before blending with PLA. The tensile properties, Izod impact strength, foam morphology, and thermal degradation of the biocomposite foams were evaluated. Cytotoxicity and in vitro degradation were tested to determine the potential of the biocomposite foam for use as a scaffold in tissue engineering. Silane treatment improved mechanical properties by increasing the interfacial adhesion between PLA and RWS. The density and void fraction of the foam samples had a greater effect on mechanical properties than pore size. Proliferation of MG‐63 cells increased with culture time, indicating that the foam samples were not cytotoxic. Promising samples were tested for degradation in a lysozyme/phosphate‐buffered saline and showed a slow rate of in vitro degradation. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 48259.

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Section: Methodsmentioning

confidence: 99%

Biocomposite foams from poly(lactic acid) and rubber wood sawdust: Mechanical properties, cytotoxicity, and in vitro degradation

Sungsee

Tanrattanakul

2019

J of Applied Polymer Sci

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“…To modify silk fibroin scaffolds, the decellularized pulp solution was prepared at a concentration of 0.1 mg/mL in 0.1% sodium hypochlorite. Fibronectin solution was prepared at a concentration of 0.1 mg/mL in deionized water . A combination of decellularized pulp and fibronectin was prepared at a ratio of 50:50.…”

Section: Methodsmentioning

confidence: 99%

“…The decellularized tissue effectively induced tissue regeneration . However, the use of decellularized pulp tissue was rarely reported . The components of decellularized pulp tissue are microenvironments that act as clues for tissue regeneration, for instance collagen type I, collagen type II, and fibronectin .…”

Section: Introductionmentioning

confidence: 99%

“…12 However, the use of decellularized pulp tissue was rarely reported. 13,14 The components of decellularized pulp tissue are microenvironments that act as clues for tissue regeneration, for instance collagen type I, collagen type II, and fibronectin. 15 Therefore, the microenvironment of decellularized pulp was used for porous scaffolds of silk fibroin.…”

Section: Introductionmentioning

confidence: 99%

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Modified porous scaffolds of silk fibroin with mimicked microenvironment based on decellularized pulp/fibronectin for designed performance biomaterials in maxillofacial bone defect

Sangkert

Kamonmattayakul

Chai

et al. 2017

J Biomedical Materials Res

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Maxillofacial bone defect is a critical problem for many patients. In severe cases, the patients need an operation using a biomaterial replacement. Therefore, to design performance biomaterials is a challenge for materials scientists and maxillofacial surgeons. In this research, porous silk fibroin scaffolds with mimicked microenvironment based on decellularized pulp and fibronectin were created as for bone regeneration. Silk fibroin scaffolds were fabricated by freeze-drying before modification with three different components: decellularized pulp, fibronectin, and decellularized pulp/fibronectin. The morphologies of the modified scaffolds were observed by scanning electron microscopy. Existence of the modifying components in the scaffolds was proved by the increase in weights and from the pore size measurements of the scaffolds. The modified scaffolds were seeded with MG-63 osteoblasts and cultured. Testing of the biofunctionalities included cell viability, cell proliferation, calcium content, alkaline phosphatase activity (ALP), mineralization and histological analysis. The results demonstrated that the modifying components organized themselves into aggregations of a globular structure. They were arranged themselves into clusters of aggregations with a fibril structure in the porous walls of the scaffolds. The results showed that modified scaffolds with a mimicked microenvironment of decellularized pulp/fibronectin were suitable for cell viability since the cells could attach and spread into most of the pores of the scaffold. Furthermore, the scaffolds could induce calcium synthesis, mineralization, and ALP activity. The results indicated that modified silk fibroin scaffolds with a mimicked microenvironment of decellularized pulp/fibronectin hold promise for use in tissue engineering in maxillofacial bone defects. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1624-1636, 2017.

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“…Chitosan and PLLA are biocompatible, biodegradable and bioabsorbable materials [4]- [7] which serve as a structure for the undifferentiated cells and osteogenic potential as growth factors, inducing the differentiation of osteoblasts [8]. Chitosan has a hydrophilic surface which makes it biocompatible [9]- [11].…”

Section: Introductionmentioning

confidence: 99%

Development of Chitosan/Poly(L-Lactide)/Multiwalled Carbon Nanotubes Scaffolds for Bone Tissue Engineering

Orozco¹,

Jiménez-Vega²,

Martel-Estrada³

et al. 2016

OJRM

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This work focuses to improve the mechanical properties and investigates the growth of osteoblasts on a degradable chitosan/poly(L-lactide)/carbon nanotubes composite for tissue engineering. The morphological and mechanical properties characterizations were performed using scanning electronic microscopy (SEM) and rheometrics analysis system (RSA). Osteoblasts differentiation was determined by alkaline phosphatase activity, total number cells by an Alamar Blue assay, cell attachment and proliferation were visualized qualitatively using SEM, mineralization was characterized by transformed infrared spectroscopy, energy dispersive spectroscopy, and X-ray diffraction. The results suggest that the chitosan/poly(L-lactide)/multiwalled carbon nanotubes composites exhibit the ability to promote cell adhesion, proliferation, and differentiation on their surface.

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A biofunctional-modified silk fibroin scaffold with mimic reconstructed extracellular matrix of decellularized pulp/collagen/fibronectin for bone tissue engineering in alveolar bone resorption

Cited by 26 publications

References 13 publications

Biocomposite foams from poly(lactic acid) and rubber wood sawdust: Mechanical properties, cytotoxicity, and in vitro degradation

Biocomposite foams from poly(lactic acid) and rubber wood sawdust: Mechanical properties, cytotoxicity, and in vitro degradation

Modified porous scaffolds of silk fibroin with mimicked microenvironment based on decellularized pulp/fibronectin for designed performance biomaterials in maxillofacial bone defect

Development of Chitosan/Poly(L-Lactide)/Multiwalled Carbon Nanotubes Scaffolds for Bone Tissue Engineering

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