A Biofilm Approach to Detect Bacteria on Removed Spinal Implants

Sampedro, Marta Fernández; Huddleston, P.M.; Piper, Kerryl E.; Karau, Melissa J.; Dekutoski, Mark B.; Yaszemski, Michael J.; Currier, Bradford L.; Mandrekar, Jayawant N.; Osmon, Douglas R.; McDowell, Andrew; Patrick, Sheila; Steckelberg, James M.; Patel, Robin

doi:10.1097/brs.0b013e3181c3b2f3

Cited by 137 publications

(83 citation statements)

References 19 publications

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“…P ropionibacterium species are often grown on culture of specimens obtained at the time of orthopaedic revision surgery. The pathogenicity of this organism has been demonstrated for patients who have undergone shoulder implant surgery [1][2][3][4][5][6][7][8][9][10][11][12][13][14] , hip or knee arthroplasty [15][16][17][18][19][20][21][22] , or spine surgery [23][24][25][26][27][28][29][30][31][32][33][34][35][36][37] . While many cases of Propionibacterium infections are attributed to Propionibacterium acnes, other species of Propionibacterium have been recovered from revision surgical sites 38 .…”

Section: Discussionmentioning

confidence: 99%

Origin of Propionibacterium in Surgical Wounds and Evidence-Based Approach for Culturing Propionibacterium from Surgical Sites

Matsen

Butler-Wu

Carofino³

et al. 2013

The Journal of Bone & Joint Surgery

141

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Section: Discussionmentioning

confidence: 99%

Origin of Propionibacterium in Surgical Wounds and Evidence-Based Approach for Culturing Propionibacterium from Surgical Sites

Matsen

Butler-Wu

Carofino³

et al. 2013

The Journal of Bone & Joint Surgery

141

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“…22 Piper et al 23 also reported a significant difference between SC and periprosthetic TCs (66.7% vs. 54.5%, p ¼ 0.046) for the detection of definite prosthetic shoulder infection. Sampedro et al 24 presented an improved pathogen detection from the surface of spinal implants with the SC method. To exclude contamination bacterial growth had to be present in two or more periprosthetic tissue specimens in all of these studies.…”

Section: Discussionmentioning

confidence: 99%

Sonication cultures of explanted components as an add‐on test to routinely conducted microbiological diagnostics improve pathogen detection

Holinka

Bauer

Hirschl

et al. 2010

Journal Orthopaedic Research

138

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The purpose of this study was to improve the pathogen detection in prosthetic joint infections, particularly to evaluate the feasibility of the sonication culture method in the clinical routine. Explanted components of all patients with presumptive prosthetic or implant infection were sonicated separately in sterile containers to dislodge the adherent bacteria from the surfaces and cultured. The results of sonication culture were compared to the conventional tissue culture. We investigated 60 consecutive patients with loosening of the prostheses or implants Forty patients had septic and 20 aseptic loosening (24 knee prostheses, 21 hip prostheses, 6 mega-prostheses, 2 shoulder prostheses, 6 osteosynthesis, 1 spinal instrumentation). The sensitivity of sonication fluid culture was 83.3%, of single positive tissue culture was 72.2% and 61.1% when two or more cultures yielded the same microorganism. In patients receiving antibiotic therapy the sensitivity was 65.9%, 57.5%, and 42.5%, respectively. Pathogens detected in a single tissue culture as well as in sonication culture yielded a significantly higher rate of prosthetic infection than conventional tissue culture alone ( p ¼ 0.008), even in patients receiving continuous antibiotic therapy before explantation ( p ¼ 0.016). The sonication method represents an essential add-on in pathogen detection compared to conventional tissue culture. ß

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“…The conclusions showed that the preliminary implant preparation by sonication followed by culture improved the sensitivity of biofilm bacterial detection and identification (53).…”

Section: Quantification and Visualization Of Microbial Biofilmmentioning

confidence: 95%

Microbial biofilm in human health - an updated theoretical and practical insight

Licker

Moldovan

Hogea

et al. 2017

Revista Romana De Medicina De Laborator

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A Biofilm Approach to Detect Bacteria on Removed Spinal Implants

Abstract: Implant sonication followed by culture is more sensitive than peri-implant tissue culture for the microbiologic diagnosis of spinal implant infection.

Cited by 137 publications

References 19 publications

Origin of Propionibacterium in Surgical Wounds and Evidence-Based Approach for Culturing Propionibacterium from Surgical Sites

Origin of Propionibacterium in Surgical Wounds and Evidence-Based Approach for Culturing Propionibacterium from Surgical Sites

Sonication cultures of explanted components as an add‐on test to routinely conducted microbiological diagnostics improve pathogen detection

Microbial biofilm in human health - an updated theoretical and practical insight

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