A biocleavable pullulan-based vector via ATRP for liver cell-targeting gene delivery

Yang, Xiaolong; Niu, Yan-Lan; Zhao, Nana; Mao, Chun; Xu, Fu‐Jian

doi:10.1016/j.biomaterials.2014.01.036

Cited by 105 publications

(102 citation statements)

References 33 publications

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“…Hemolysis induced through high concentration (5 mg/mL) of SA-BSPs copolymer micelles probably resulted from membrane destruction induced by SA-BSPs copolymer micelles [37]. Hemolysis of biomaterials is limited to 5% [38]. Thus, SA-BSPs copolymer micelles showed good blood compatibility below 3 mg/mL concentration, indicating that SA-BSPs copolymer was biocompatible and safe.…”

Section: In Vitro Hemolysis Assaymentioning

confidence: 99%

Docetaxel-Loaded Self-Assembly Stearic Acid-Modified Bletilla striata Polysaccharide Micelles and Their Anticancer Effect: Preparation, Characterization, Cellular Uptake and In Vitro Evaluation

et al. 2016

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Poorly soluble drugs have low bioavailability after oral administration, thereby hindering effective drug delivery. A novel drug-delivery system of docetaxel (DTX)-based stearic acid (SA)-modified Bletilla striata polysaccharides (BSPs) copolymers was successfully developed. Particle size, zeta potential, encapsulation efficiency (EE), and loading capacity (LC) were determined. The DTX release percentage in vitro was determined using high performance liquid chromatography (HPLC). The hemolysis and in vitro anticancer activity were studied. Cellular uptake and apoptotic rate were measured using flow cytometry assay. Particle size, zeta potential, EE and LC were 125.30 ± 1.89 nm, −26.92 ± 0.18 mV, 86.6% ± 0.17%, and 14.8% ± 0.13%, respectively. The anticancer activities of DTX-SA-BSPs copolymer micelles against HepG2, HeLa, SW480, and MCF-7 (83.7% ± 1.0%, 54.5% ± 4.2%, 48.5% ± 4.2%, and 59.8% ± 1.4%, respectively) were superior to that of docetaxel injection (39.2% ± 1.1%, 44.5% ± 5.3%, 38.5% ± 5.4%, and 49.8% ± 2.9%, respectively) at 0.5 µg/mL drug concentration. The DTX release percentage of DTX-SA-BSPs copolymer micelles and docetaxel injection were 66.93% ± 1.79% and 97.06% ± 1.56% in two days, respectively. Cellular uptake of DTX-FITC-SA-BSPs copolymer micelles in cells had a time-dependent relation. Apoptotic rate of DTX-SA-BSPs copolymer micelles and docetaxel injection were 73.48% and 69.64%, respectively. The SA-BSPs copolymer showed good hemocompatibility. Therefore, SA-BSPs copolymer can be used as a carrier for delivering hydrophobic drugs.

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Section: In Vitro Hemolysis Assaymentioning

confidence: 99%

Docetaxel-Loaded Self-Assembly Stearic Acid-Modified Bletilla striata Polysaccharide Micelles and Their Anticancer Effect: Preparation, Characterization, Cellular Uptake and In Vitro Evaluation

et al. 2016

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“…content and molecular weight showed superior gene delivery efficacies in vitro. Interestingly, the gene delivery in hepatocytes was inhibited in the presence of asialofetuin [53].…”

Section: Pullulan-based Nanoparticlesmentioning

confidence: 97%

Carbohydrate-Based Materials for Targeted Delivery of Drugs and Genes to the Liver

Ahmed

Narain

2015

Nanomedicine (Lond.)

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The insult to liver by toxic materials leads to cirrhosis, hepatitis and cancer. Upon administration, drugs accumulate in liver, which is systemically cleared by reticuloendothelial system. However, specific targeting of drugs to liver is a serious challenge. Specific delivery of molecules to hepatocytes is accomplished by targeting cell surface lectins, asialoglycoprotein receptors. Asialofetuin, N-acetyl glucosamine and galactose are high-affinity ligands of asialoglycoprotein receptors. The bioconjugation of drugs, fluorescent molecules and gene delivery vectors with lectin-targeting agents, and their delivery in liver hepatocytes, is discussed. Mannose and N-acetyl glucosamine conjugates are evaluated for their delivery to hepatic stellate and kupffer cells. The glycosylated gene and drug delivery vectors in clinical trials are outlined.

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“…Due to its excellent biological and physicochemical features, namely biodegradability, non-immunogenicity, non-mutagenicity, non-toxicity and non-carcinogenicity, especially its water solubility and low viscosity, this polymer has become an attractive option for many pharmaceutical applications and chemical manipulations (Ambattu & Rekha, 2015;Belbekhouche, Ali, Dulong, Picton & Le Cerf, 2011;Ghimici, Constantin & Fundueanu, 2010;Shingel, 2004;Wang, Cui, Bao, Xing & Hao, 2014;Wang, Dou & Bao, 2014). (ii) Pullulan exhibits extremely high flexibility in aqueous solutions and has inherent liver affinity as the substrate of ASGPR (Kaneo, Tanaka, Nakano & Yamaguchi, 2001;Kang, Tachibana, Kamata, Mahara, Harada-Shiba & Yamaoka, 2010;Rekha & Sharma, 2011;Wu, Zou, Deng, Cheng, Meng & Zhong, 2013;Yang, Niu, Zhao, Mao & Xu, 2014). (iii)…”

Section: Introductionmentioning

confidence: 99%

Efficient delivery of paclitaxel into ASGPR over-expressed cancer cells using reversibly stabilized multifunctional pullulan nanoparticles

Huang

Wang

Ling

et al. 2017

Carbohydrate Polymers

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A biocleavable pullulan-based vector via ATRP for liver cell-targeting gene delivery

Cited by 105 publications

References 33 publications

Docetaxel-Loaded Self-Assembly Stearic Acid-Modified Bletilla striata Polysaccharide Micelles and Their Anticancer Effect: Preparation, Characterization, Cellular Uptake and In Vitro Evaluation

Docetaxel-Loaded Self-Assembly Stearic Acid-Modified Bletilla striata Polysaccharide Micelles and Their Anticancer Effect: Preparation, Characterization, Cellular Uptake and In Vitro Evaluation

Carbohydrate-Based Materials for Targeted Delivery of Drugs and Genes to the Liver

Efficient delivery of paclitaxel into ASGPR over-expressed cancer cells using reversibly stabilized multifunctional pullulan nanoparticles

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