A bioautographic agar overlay method for the detection of antifungal compounds from higher plants

Rahalison, Lila; Hamburger, Matthias; Hostettmann, Kurt; Monod, Michel; Frenk, E.

doi:10.1002/pca.2800020503

Cited by 303 publications

(222 citation statements)

References 9 publications

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“…7 This polyketide presents sclerotinin skeleton, whose biosynthesis was proposed by Barber et al 8 and Tokoroyama and Kubota. 10 The antifungal activity of compounds 1-3 against the phytopatogenic fungi Cladosporium cladosporioides and C. sphaerospermum was evaluated by bioautography 11,12 and the detection limit for the compounds 1 and 2 was 1.0 μg, the same as for the positive control nystatin. Citotoxity of the compounds 1, 2 and 3 against human cervical tumor cell line (HeLa) were tested using the MTT assay.…”

Section: Resultsmentioning

confidence: 99%

New bioactive metabolites produced by Phomopsis cassiae, an endophytic fungus in Cassia spectabilis

Silva¹,

Teles²,

Trevisan³

et al. 2005

J. Braz. Chem. Soc.

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Dois novos metabólitos, 2,4-diidroxi-5,6-dimetil benzoato de etila (1) e phomopsilactona (2) foram isolados de Phomopsis cassiae, um fungo endofítico de Cassia spectabilis. As estruturas destes compostos foram elucidadas por dados espectrométricos de 1D e 2D RMN, EM e IV. As substâncias 1 e 2 exibiram forte atividade antifúngica contra os fungos fitopatogênicos Cladosporium cladosporioides e C. sphaerospermum, bem como citotoxidade contra a linhagem celular de tumor cervical humano (HeLa), em experimentos in vitro.Two new metabolites, ethyl 2,4-dihydroxy-5,6-dimethylbenzoate (1) and phomopsilactone (2) were isolated from Phomopsis cassiae, an endophytic fungus in Cassia spectabilis. Their structures were elucidated by 1D and 2D NMR, MS and IR spectral data. Compounds 1 and 2 displayed strong antifungal activity against the phytopatogenic fungi Cladosporium cladosporioides and C. sphaerospermum, as well as cytotoxicity against human cervical tumor cell line (HeLa), in in vitro assays.

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Section: Resultsmentioning

confidence: 99%

New bioactive metabolites produced by Phomopsis cassiae, an endophytic fungus in Cassia spectabilis

Silva¹,

Teles²,

Trevisan³

et al. 2005

J. Braz. Chem. Soc.

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show abstract

“…The TLC bioautography test (Rahalison et al, 1991) was performed to determine qualitatively the antibiotic activity of all fractions. The TLC plates were each placed in a Petri dish 90 mm in diameter containing 20 mL of NA plus 1 mL cell suspension of X. axonopodis (10 8 CFU mL -1 , D.O 0.1, λ= 590 nm) in the log phase.…”

Section: Evaluation Of Antibiotic Activity Tlc Bioautography Methodsmentioning

confidence: 99%

Activity of extracellular compounds of Pseudomonas sp. against Xanthomonas axonopodis in vitro and bacterial leaf blight in eucalyptus

Lopes¹,

Oliveira²,

Beranger³

et al. 2012

Trop. plant pathol.

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Bacterial leaf blight caused by Xanthomonas axonopodis is a major problem in the production of eucalyptus seedlings. The pathogen causes leaf lesions that reduce the photosynthetic area and limit plant growth. The objective of this study was to evaluate the antibiotic activity of secondary metabolic compounds of a Pseudomonas sp. against X. axonopodis, following extraction with dichloromethane and fractionation by vacuum liquid chromatography. The antibiotic activity of an ethyl acetate phase (F3 fraction) was determined by agar diffusion tests and bioautography. The minimum inhibitory concentration of F3 fraction and the action of F3 on the cell morphology of X. axonopodis was determined by scanning electron microscopy. Electron microscopy revealed changes in exopolysaccharides and morphology of treated cells. When eucalyptus seedlings were sprayed with the F3 fraction before or after inoculation with X. axonopodis in a greenhouse experiment the number of leaf lesions was respectively 93.9% and 89.7% lower than in untreated control plants. High antibiotic activity of the F3 fraction against X. axonopodis and leaf blight suggests that it has potential to control the disease in eucalyptus seedlings.

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“…Such substances do not diffuse in agar, a circumstance that can result in false negatives. Prior work from our group with a modification of Rahalison's technique (Rahalison et al 1991;Larramendi et al 1998) has shown that Bioautography on TLC is particularly useful to assay bioactive compounds present in complex mixtures, as shown in Figure 4, with a quantitative perspective.…”

Section: Antifungal Testsmentioning

confidence: 99%

Morphological and chemical diversity in the Type IV glandular trichomes of Solananeae (S. sisymbrifolium and N. glauca) as germplasm resources for agricultural and food uses

Cesio¹,

Dutra²,

Moyna³

et al. 2006

Electron. J. Biotechnol.

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A bioautographic agar overlay method for the detection of antifungal compounds from higher plants

Cited by 303 publications

References 9 publications

New bioactive metabolites produced by Phomopsis cassiae, an endophytic fungus in Cassia spectabilis

New bioactive metabolites produced by Phomopsis cassiae, an endophytic fungus in Cassia spectabilis

Activity of extracellular compounds of Pseudomonas sp. against Xanthomonas axonopodis in vitro and bacterial leaf blight in eucalyptus

Morphological and chemical diversity in the Type IV glandular trichomes of Solananeae (S. sisymbrifolium and N. glauca) as germplasm resources for agricultural and food uses

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