A bioactivity based comparison of Echinacea purpurea extracts obtained by various processes

Yıldız, Ece; Karabulut, Dilan; Yeşil-Çeliktaş, Özlem

doi:10.1016/j.supflu.2014.02.005

Cited by 13 publications

(7 citation statements)

References 24 publications

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“…The difference between the results presented in the current study and the literature data [ 39 ] can be explained, most likely, by the influence of environmental factors and cultivar quality, leading us to the conclusion that E. purpurea samples grown under native conditions exhibit a lower intake of phenolic compounds. At the same time, the selected extraction methods (using milder conditions and “greener” solvents) could also negatively influence the total phenolic content.…”

Section: Discussioncontrasting

confidence: 97%

“…11 mg catechin equivalents/100 g ethanolic extracts [ 38 ] and between 10 and 33 quercitin equivalents/g dry weight for different parts of E. purpurea extracts grown in supplemented soil [ 40 ]. The total flavonoid content observed in this study was higher than the flavonoid contents reported by other authors [ 39 ], leading to the conclusion that the selected methods positively influenced the total flavonoid content.…”

Section: Discussioncontrasting

confidence: 86%

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Phytosynthesis of Biological Active Silver Nanoparticles Using Echinacea purpurea L. Extracts

et al. 2022

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With their phytoconstituents acting as reducing and capping agents, natural extracts can be considered a viable alternative for the obtaining of metallic nanoparticles. The properties of phytosynthesized nanoparticles are dependent upon size and morphology, which, in turn, can be tailored by adjusting different parameters of the phytosynthesis process (such as the extracts’ composition). In the present study, we aimed to evaluate, for the first time in the literature, the influence of the extraction method and extract concentration on the morphological and biological properties (antioxidant and antibacterial activity) of silver nanoparticles phytosynthesized using Echinacea pupurea L. extracts. The obtained results revealed that the use of the low-concentration Echinacea hydro-alcoholic extract obtained via classical temperature extraction led to the development of nanoparticles with the smallest dimensions (less than 10 nm), compared with the use of extracts obtained with higher concentrations and the extract obtained via the microwave method. The developed nanomaterials exhibited enhanced antioxidant effects (determined via the DPPH assay) and antimicrobial properties (against Escherichia coli and Candida albicans), compared with the parent extracts.

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Section: Discussioncontrasting

confidence: 97%

Section: Discussioncontrasting

confidence: 86%

Phytosynthesis of Biological Active Silver Nanoparticles Using Echinacea purpurea L. Extracts

et al. 2022

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“…Various extraction techniques were already employed to extract bioactive compounds from E. purpurea . Classical extraction, such as solvent extraction with or without stirring, infusions, decoctions, maceration, and soxhlet [ 30 , 31 , 32 , 33 , 34 , 35 ], uses high temperatures to obtain high yields of the bioactive compounds [ 36 ]. However, it is well known that temperature can denature several compounds, reducing their biological activity.…”

Section: Introductionmentioning

confidence: 99%

“…However, it is well known that temperature can denature several compounds, reducing their biological activity. Despite the use of ultrasounds [ 37 ] and microwaves [ 30 ] present many advantages in comparison with the classical methods (e.g., less extraction time and solvent consumption and higher yield), they are also associated with degradation and loss of integrity of bioactive compounds, due to the production of radicals [ 37 ]. Besides the technique, the solvent used in the extraction also affects drastically the amount and type of bioactive compounds extracted.…”

Section: Introductionmentioning

confidence: 99%

On the Bioactivity of Echinacea purpurea Extracts to Modulate the Production of Inflammatory Mediators

Vieira

Gonçalves

Llaguno

et al. 2022

IJMS

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Inflammatory diseases are the focus of several clinical studies, due to limitations and serious side effects of available therapies. Plant-based drugs (e.g., salicylic acid, morphine) have become landmarks in the pharmaceutical field. Therefore, we investigated the immunomodulatory effects of flowers, leaves, and roots from Echinacea purpurea. Ethanolic (EE) and dichloromethanolic extracts (DE) were obtained using the Accelerated Solvent Extractor and aqueous extracts (AE) were prepared under stirring. Their chemical fingerprint was evaluated by liquid chromatography–high resolution mass spectrometry (LC-HRMS). The pro- and anti-inflammatory effects, as well as the reduction in intracellular reactive oxygen and nitrogen species (ROS/RNS), of the different extracts were evaluated using non-stimulated and lipopolysaccharide-stimulated macrophages. Interestingly, AE were able to stimulate macrophages to produce pro-inflammatory cytokines (tumor necrosis factor -TNF-α, interleukin -IL-1β, and IL-6), and to generate ROS/RNS. Conversely, under an inflammatory scenario, all extracts reduced the amount of pro-inflammatory mediators. DE, alkylamides-enriched extracts, showed the strongest anti-inflammatory activity. Moreover, E. purpurea extracts demonstrated generally a more robust anti-inflammatory activity than clinically used anti-inflammatory drugs (dexamethasone, diclofenac, salicylic acid, and celecoxib). Therefore, E. purpurea extracts may be used to develop new effective therapeutic formulations for disorders in which the immune system is either overactive or impaired.

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“…The free radical scavenging activities of extracts were determined as reported previously. 26 The extracts dissolved in 4 mL of ethanol were added to 0.5 mL of 1 mM methanolic solution of 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) (Sigma-Aldrich, USA). The contents were stirred for 15 s and then left at room temperature in the dark for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Essential Oil and Supercritical Carbon Dioxide Extract of Grapefruit Peels Formulated for Candida albicans Infections: Evaluation by an in Vitro Model to Study Fungal–Host Interactions

et al. 2022

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Resistance to currently available antifungal agents raises the need to develop alternative remedies. Candida albicans is the most common opportunistic pathogenic fungus of humans, colonizing in the genital and intestinal mucosa, skin, and oral-nasal cavity and reducing quality of life. Herein, essential oil from grapefruit (Citrus paradise) peels was obtained by hydrodistillation, and the remaining plant material was sequentially subjected to supercritical carbon dioxide (SC-CO2) extraction to determine the conditions for maximizing phenolic compounds. A statistical design was used to evaluate the effect of temperature (30, 50, 70 °C), pressure (80, 150, 220 bar), and ethanol as a cosolvent (0%, 10%, and 20% v/v). Essential oil and SC-CO2 extracts were mixed at various ratios to develop an effective antifungal formulation. Subsequently, fungal infection was modeled by coculturing C. albicans with human skin keratinocytes (HaCaT) to mimic dermal mycoses, endothelial cells (HUVEC) to evaluate vascular fate, and cervical adenocarcinoma (HeLa) cells to represent additional genital mycoses. Treatment with essential oil and extract (25:75%) formulation for 8 h exhibited slight cytotoxicity toward HeLa cells, no toxicity toward HaCaT and HUVECs, whereas inhibition of C. albicans. Considering the clinical significance, such in vitro models are essential to screen potential compounds for the treatment of opportunistic fungal infections.

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A bioactivity based comparison of Echinacea purpurea extracts obtained by various processes

Cited by 13 publications

References 24 publications

Phytosynthesis of Biological Active Silver Nanoparticles Using Echinacea purpurea L. Extracts

Phytosynthesis of Biological Active Silver Nanoparticles Using Echinacea purpurea L. Extracts

On the Bioactivity of Echinacea purpurea Extracts to Modulate the Production of Inflammatory Mediators

Essential Oil and Supercritical Carbon Dioxide Extract of Grapefruit Peels Formulated for Candida albicans Infections: Evaluation by an in Vitro Model to Study Fungal–Host Interactions

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