A bio-orthogonal functionalization strategy for site-specific coupling of antibodies on vesicle surfaces after self-assembly

Gai, Meiyu; Simon, Johanna; Lieberwirth, Ingo; Mailänder, Volker; Morsbach, Svenja; Landfester, Katharina

doi:10.1039/c9py01136f

Cited by 34 publications

(33 citation statements)

References 51 publications

(58 reference statements)

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“…Different biomacromolecules, e.g . CD11c antibody for dendritic cell targeting (Gai et al., 2020) or apolipoprotein A1 for brain endothelial cells targeting (Zensi et al., 2010), can be clicked on the EVs surface after their azidation (‐N 3 groups) via bio‐orthogonal strain‐promoted alkyne–azide cycloaddition (SPAAC) click chemistry reaction, where Cu (I) as a catalyst can be avoided.…”

Section: Resultsmentioning

confidence: 99%

Nanoalgosomes: Introducing extracellular vesicles produced by microalgae

Adamo

Fierli

Romancino

et al. 2021

J of Extracellular Vesicle

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126

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Cellular, inter‐organismal and cross kingdom communication via extracellular vesicles (EVs) is intensively studied in basic science with high expectation for a large variety of bio‐technological applications. EVs intrinsically possess many attributes of a drug delivery vehicle. Beyond the implications for basic cell biology, academic and industrial interests in EVs have increased in the last few years. Microalgae constitute sustainable and renewable sources of bioactive compounds with a range of sectoral applications, including the formulation of health supplements, cosmetic products and food ingredients. Here we describe a newly discovered subtype of EVs derived from microalgae, which we named nanoalgosomes. We isolated these extracellular nano‐objects from cultures of microalgal strains, including the marine photosynthetic chlorophyte Tetraselmis chuii, using differential ultracentrifugation or tangential flow fractionation and focusing on the nanosized small EVs (sEVs). We explore different biochemical and physical properties and we show that nanoalgosomes are efficiently taken up by mammalian cell lines, confirming the cross kingdom communication potential of EVs. This is the first detailed description of such membranous nanovesicles from microalgae. With respect to EVs isolated from other organisms, nanoalgosomes present several advantages in that microalgae are a renewable and sustainable natural source, which could easily be scalable in terms of nanoalgosome production.

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Section: Resultsmentioning

confidence: 99%

Nanoalgosomes: Introducing extracellular vesicles produced by microalgae

Adamo

Fierli

Romancino

et al. 2021

J of Extracellular Vesicle

Self Cite

126

View full text Add to dashboard Cite

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“…Another recent study established a novel method to conjugate antibodies to preformed liposomes [87]. This strategy was explored to improve the conjugation of antibodies to preformed liposomes (such as natural carriers), and to provide an alternate strategy of conjugation in the cases where prefunctionalization disrupts the liposome self-assembly process.…”

Section: Antibody-liposome Bioconjugates (Immunoliposomes)mentioning

confidence: 99%

“…Image adapted from Gai © Royal Society of Chemistry (2020) Ref. [87]. (C) (i) Schematic of the thermoresponsive AS1411 liposome with FITC-labeled secondary antibody was allowed to attach to DBCO and CD11c-conjugated liposomes under two different reaction conditions (blue versus red bars).…”

Section: Aptamer-liposome Bioconjugatesmentioning

confidence: 99%

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Recent Progress in Bioconjugation Strategies for Liposome-Mediated Drug Delivery

et al. 2020

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In nanoparticle (NP)-mediated drug delivery, liposomes are the most widely used drug carrier, and the only NP system currently approved by the FDA for clinical use, owing to their advantageous physicochemical properties and excellent biocompatibility. Recent advances in liposome technology have been focused on bioconjugation strategies to improve drug loading, targeting, and overall efficacy. In this review, we highlight recent literature reports (covering the last five years) focused on bioconjugation strategies for the enhancement of liposome-mediated drug delivery. These advances encompass the improvement of drug loading/incorporation and the specific targeting of liposomes to the site of interest/drug action. We conclude with a section highlighting the role of bioconjugation strategies in liposome systems currently being evaluated for clinical use and a forward-looking discussion of the field of liposomal drug delivery.

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“…13,14 The compartmentalized nature of polymersomes facilitates encapsulation of various types of cargo within their inner lumen, protecting them from unwanted degradation whilst facilitating their delivery to cells through passive or active uptake (using homing motifs such as peptides or antibodies). 4,15 The shape effect of course also holds for polymersomes, and this has inspired researchers to develop effective methods for the construction of non-spherical vesicular structures.…”

Section: Introductionmentioning

confidence: 99%

Influence of surface charge on the formulation of elongated PEG-b-PDLLA nanoparticles

2020

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A bio-orthogonal functionalization strategy for site-specific coupling of antibodies on vesicle surfaces after self-assembly

Abstract: Attaching targeting ligands on the surface of self-assembled drug delivery systems is one of the key requests for a controlled transport of the drug to a desired location.

Cited by 34 publications

References 51 publications

Nanoalgosomes: Introducing extracellular vesicles produced by microalgae

Nanoalgosomes: Introducing extracellular vesicles produced by microalgae

Recent Progress in Bioconjugation Strategies for Liposome-Mediated Drug Delivery

Influence of surface charge on the formulation of elongated PEG-b-PDLLA nanoparticles

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