A bimodal type of AgPd Plasmonic Blackbody Nanozyme with boosted catalytic efficacy and synergized photothermal therapy for efficacious tumor treatment in the second biological window

Abstract

Nanozymes are promising for precise cancer treatment, but are typically limited in terms of the low catalytic efficiency and the complexity in tumor microenvironment (TME). Herein, we describe a bimodal type of AgPd plasmonic blackbody (AgPd PB) nanozyme of compact sizes (< 30 nm), which presents not only boosted enzyme efficacy but also efficient photothermal therapy (PTT) for synergized therapy through tissue-penetrating light in the second biological window (1000–1700 nm). The synthesized hyperbranched AgPd PB nanozymes possess intense and broadband localized surface plasmonic resonance absorption of 400–1300 nm, entailing prominent photothermal efficiency (η = 45.1% at 1064 nm) for PTT. Importantly, PTT was found to significantly boost the nanozyme efficacy of both catalase (CAT) and peroxidase (POD) processes, which correspondingly decompose H2O2 to into O2 to relieve tumor hypoxia, and activate H2O2 to generate oxidative •OH radical. While the generated •OH was found to be able to minimize heat shock proteins (HSPs), which plays a vital role to counterbalance PTT effect both in vitro and in vivo. As compared to control ground without treatment, the synergized nanozyme and PTT activities resulted in about 7-fold reduction of tumor volume, thus elevating the survival rate from 0 to 80% at 30 days posttreatment. Besides the synergistic therapy, the AgPd PB nanozyme were shown to own fluorescence, computed tomography (CT), and photoacoustic (PA) imaging abilities, thus having implications for uses in imaging-guided precise cancer therapy. This study provides a paradigm of TME responsive theranostics under NIR-II light irradiation. Graphical Abstract

Carbon dots-mediated synthesis of gold nanodendrites with extended absorption into NIR-II window for in vivo photothermal therapy

“…S13b). However, at the same thickness, the temperature increment caused by 1064 nm laser was higher than that of 808 nm laser, implying the stronger tissue penetration ability of NIR-II light than that of NIR-I light [ 35 ]. In order to better understand the photothermal source of AuNDs, the absorption spectrum and electric field distribution of AuNDs were calculated by Finite-Difference Time-Domain (FDTD) software [ 36 ].…”

pH Switchable Nanozyme Platform for Healing Skin Tumor Wound Infected with Drug‐Resistant Bacteria

“…To characterize O 2 production, we used an O 2 level indicator of [Ru(dpp) 3 ]Cl 2 (RDPP), which exhibited decreased fluorescence in the presence of O 2 . [ 20 ] Figure 2E shows that RDPP fluorescence was visibly quenched in the presence of CaO 2 , and evident gas bubbles were simultaneously observed, indicating that O 2 was effectively released from CaO 2 upon contact with water. Furthermore, the color of the aqueous solution of potassium permanganate (KMnO 4 ) faded with CaO 2 addition, similar to the effect of H 2 O 2 (Figure 2F), further confirming the generation of H 2 O 2 from CaO 2 .…”

Smart Biomimetic Nanozymes for Precise Molecular Imaging: Application and Challenges

“…In addition, due to its inherently short cycle time, it is more difficult to obtain high-resolution images [ 132 ]. Even though conjugated macromolecules can overcome this problem with gadolinium chelates, live ions may increase the possibility of side effects [ 133 , 134 ]. Therefore, it is vital to develop new MRI contrast agents that can improve the imaging sensitivity and accuracy of disease diagnosis.…”