A bi-enzymatic whole cell conductometric biosensor for heavy metal ions and pesticides detection in water samples

Chouteau, Céline; Dzyadevych, S. V.; Durrieu, Claude; Chovelon, Jean‐Marc

doi:10.1016/j.bios.2004.09.032

Cited by 182 publications

(78 citation statements)

References 29 publications

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“…Their robust and simple structure, their long term stability and reliability, and easy fabrication have attracted much attention from the sensor research community. Applications that have been proposed for these microelectrodes include measurement of the concentration of calcium [1], potassium [2], p(CO2) [3], pH [4], ethanol/methanol in gasoline [5], heavy metals [6], nitrate [7], urea [8][9], glucose [10], total prostate-specific antigen (PSA) [11], bacteria [12], red blood cells (hematocrit) [13], and detection of specific sequences of DNA [14]. In some applications, the measurement of a physical magnitude such as dielectric constant or conductivity of the media provides direct information of the analyte concentration [5,13].…”

Section: Introductionmentioning

confidence: 99%

“…In some applications, the measurement of a physical magnitude such as dielectric constant or conductivity of the media provides direct information of the analyte concentration [5,13]. In other applications, the deposition of a membrane or functional monolayer on top of the electrodes as a recognition element is necessary to obtain sensitivity and selectivity to particular species [1][2][3][4][6][7][8][9][10][11][12][13][14]. Different arrangements of electrodes (i.e.…”

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confidence: 99%

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Polysilicon interdigitated electrodes as impedimetric sensors

Rica

Fernández‐Sánchez

Baldi

2006

Electrochemistry Communications

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The suitability of polysilicon as a material for the fabrication of interdigitated electrodes and their application to the development of sensors is studied in this work. The main interest in using this material lies in the possibility of obtaining integrated sensors with commercial CMOS technologies and simple post-processing steps. Electrodes with 3μm finger width and 3, 10, and 20μm spacing were fabricated and characterised. Conductivity measurements in the range from 4 to 50μS/cm yielded a linear response with cell constants of 0.04cm prostate-specific antigen (PSA) [11], bacteria [12], red blood cells (hematocrit) [13], and detection of specific sequences of DNA [14]. In some applications, the measurement of a physical magnitude such as dielectric constant or conductivity of the media provides direct information of the analyte concentration [5,13]. In other applications, the deposition of a membrane or functional monolayer on top of the electrodes as a recognition element is necessary to obtain sensitivity and selectivity to particular species [1][2][3][4][6][7][8][9][10][11][12][13][14]. Different arrangements of electrodes (i.e. different number of electrodes and geometries) may be used for impedimetric measurements. Among them, interdigitated electrodes have significant advantages for certain applications: 1) their low cell constant (resistance to resistivity ratio) permit the measurement of very low conductivity solutions, 2) measurement of dielectric properties is possible thanks to a high inter-electrode capacitance (higher than stray capacitance even for conductive substrates), 3) the short penetration depth 3 of electric fields make them less dependent on the measurement cell geometry and allow the use of thin membranes for tailoring selectivity.In this paper the suitability of polysilicon as a material for the fabrication of interdigitated electrodes and their application to the development of sensors is studied. For example, the feasibility of measuring solution conductivity and permittivity with polysilicon electrodes is demonstrated. The main interest in using this material lies in the possibility of fabricating the electrodes with commercial CMOS technologies and simple post-processing steps, and therefore being able to integrate them as sensors in a more complex system-onchip. Another interesting advantage is that this material can be easily modified following a one-step silanisation reaction. In this way, robust immobilisation of either biomolecules or membranes can be carried out on to both the electrode fingers and the area in between them with the aim of developing a sensor device. As an example, a fully functionalised biosensor for the detection of urea is also presented.The basic structure of the interdigitated electrodes is depicted in Fig. 1. The polysilicon traces forming the interdigitated geometry are separated from the silicon substrate by a thick silicon dioxide layer. represented by R sol and C so l . From these components, the resistivity ρ (or the conductivity σ) and th...

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

Polysilicon interdigitated electrodes as impedimetric sensors

Rica

Fernández‐Sánchez

Baldi

2006

Electrochemistry Communications

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“…Yerel iletkenlik değişimleri alg alkalin fosfataz ve asetilkolin esteraz aktiviteleri ile gerçekleşir. Pestisitler için iç deneyler paratiyon metil ve karbofuranın tersine paraokson metilin Chorella vulgaris asetilkolin esterazı inhibe ettiğini gösteriyor [48].…”

Section: Hücre Bazlı Biyosensörlerunclassified

Pesti̇si̇t Anali̇zleri̇nde Elektroki̇myasal Bi̇yosensörleri̇n Kullanimi

Pagano¹

2016

Ömer Halisdemir Üniversitesi Mühendislik Bilimleri Dergisi

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ÖZETPestisitler, tarımsal, endüstriyel, evsel ve savaş amaçlarıyla sık olarak kullanılan kimyasallardır. Tarımda kullanılmasındaki artıştan dolayı, pestisitler en önemli çevresel kirleticiler arasında yer almaktadır. Pestisitler amperometrik, potansiyometrik, konduktometrik transduser ve alan etkili transistörler kullanılarak tespit edilebilir. Pestisit tayini için geliştirilen biyosensörlerde; enzim, antibadi, hücre ve DNA teknikleri kullanılmıştır. Pestisitlerin enzimatik tespiti, seçilen enzimin aktivitesinin inhibisyonuna dayalıdır. Enzimlerin dışında canlı mikroorganizmalar (alg, bakteri, maya ve funguslar) biyosensörler için biyokatalitik element olarak kullanılabilir. Ayrıca pestisit analizlerinde, guanin oksidasyonuna dayalı DNA biyosensörleri de kullanılmaktadır. Biyosensörler, pestisitlerin ölçüldüğü HPLC/DAD, LC/MS/MS ve GC/MS gibi ileri kimyasal analiz teknikleri ile karşılaştırıldığında hassas bulunmuşlardır. Bu çalışma, günümüze dek pestisit analizlerinde biyosensör kullanımı ile ilgili bilimsel literatürü irdelemekte ve daha önce yapılan metodları karşılaştırmaktadır. APPLICATION OF ELECTROCHEMICAL BIOSENSORS IN PESTICIDE ANALYSIS ABSTRACTPesticides are agents widely used for agricultural, industrial, household and warfare purposes. Because of their increasing use in agriculture, pesticides are among the most important environmental pollutants. Pesticides can be detected by using amperometric, potantiometric, conductometric transduser and field effect transistor. Enzyme, antibody, cell and DNA techniques have been used in biosensors which were developed for pesticide analysis. The enzymatic detection of pesticides are based on inhibition of enzymatic activity of selected enzymes. Apart from enzymes, living microorganisms (alg, bacteria, yeast and fungus) can be used as an biocatalitic element for biosensors. DNA biosensors based on guanin oxidation are also used in pesticide analysis. Biosensors, when compared with advanced analytical techniques such as HPLC/DAD, LC/MS/MS and GC/MS have been found more sensitive. This study examines the literature regarding the use of biosensors in pesticide analysis and compares the previously used methods.

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“…Conductometric biosensors use immobilized Chlorella vulgaris microalgae as bioreceptors. Chouteau et al (2005) immobilized algae inside bovine serum albumin membranes reticulated with glutaraldehyde vapors deposited on interdigitated conductometric electrodes. Local conductivity variations caused by algae alkaline phosphatase and acetylcholinesterase activities could be detected.…”

Section: Biosensors Used For the Detection Of Heavy Metalsmentioning

confidence: 99%