A benzoboroxole-functionalized monolithic column for the selective enrichment and separation of cis-diol containing biomolecules

Abstract

A benzoboroxole-functionalized monolithic column was synthesized, which exhibited the best specificity and affinity towards cis-diol containing biomolecules as compared with the boronate affinity monolithic columns reported as well as significant secondary separation capability under acidic conditions.

Synthesis of boronate-silica hybrid affinity monolith via a one-pot process for specific capture of glycoproteins at neutral conditions

“…Boronic acid functionalized monoliths have recently gained rising attentions [30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45][46], and a lots of attempts have been made to design boronate affinity monolith with desired properties for glycoproteomic researches. A variety of boronate affinity monoliths have been developed for specific capture of glycopeptides and glycoproteins [38][39][40][41][42][43][44].…”

“…A variety of boronate affinity monoliths have been developed for specific capture of glycopeptides and glycoproteins [38][39][40][41][42][43][44]. Different from regular polymer-based boronate affinity monoliths, an inorganic-organic hybrid boronate affinity monolith synthesized via a "one-pot" process was brought into sight by Lin [44], and the monolith was successfully applied to isolation and enrichment of glycoproteins.…”

“…A lowered binding pH not only is favorable for protecting the hybrid monolith, but also can avoid the risk of protein degradation when applied monolith to separations of glycoproteins. A number of strategies have been proposed to reduce the binding pH [34,35,41,42], and the resulted boronate affinity monoliths exhibit excellent specificity toward cis-diol-containing biomolecules under neutral conditions. A hybrid boronate affinity monolith was subsequently reported by Liu [45], which exhibited selectivity of cis-diol containing compounds under neutral pH conditions.…”

Coordination Networks Based on Boronate and Benzoxaborolate Ligands

“…In most cases, the organoboron function is exposed at the periphery of the material so that its intrinsic reactivity can be exploited to provide a given property to the material (e.g., capacity to bind to diols). This is for example the case for polymers developed for the detection of dopamine [12], for chromatographic columns designed for the separation of sugars [11,13], and for mesoporous silica nanoparticles allowing the controlled release of insulin [14]. Another possibility which has been looked into consists of using the boronic acids themselves as building blocks for the preparation of functional (nano)materials.…”

Preparation of Molecularly Imprinted Polymers Functionalized with Core–shell Magnetic Nanoparticles for the Recognition of Glycoprotein

“…This reversible binding has made boronic acids excellent affinity ligands for creating functionalized materials [4][5][6].…”